Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00060 and PS417_00065 are separated by 81 nucleotides PS417_00065 and PS417_00070 are separated by 40 nucleotides PS417_00070 and PS417_00075 are separated by 136 nucleotides PS417_00075 and PS417_00080 are separated by 17 nucleotides
PS417_00060: PS417_00060 - glycyl-tRNA synthetase, at 14,341 to 15,294
_00060
PS417_00065: PS417_00065 - 3-methyladenine DNA glycosylase, at 15,376 to 15,933
_00065
PS417_00070: PS417_00070 - lipid A biosynthesis lauroyl acyltransferase, at 15,974 to 16,861
_00070
PS417_00075: PS417_00075 - hypothetical protein, at 16,998 to 17,309
_00075
PS417_00080: PS417_00080 - potassium transporter peripheral membrane component, at 17,327 to 18,700
_00080
Position (kb) 15
16
17 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 15.309 kb on - strand at 15.375 kb on + strand at 15.412 kb on + strand at 15.412 kb on + strand at 15.412 kb on + strand at 15.421 kb on + strand at 15.422 kb on - strand at 15.524 kb on + strand, within PS417_00065 at 15.767 kb on + strand, within PS417_00065 at 16.856 kb on + strand at 16.924 kb on + strand at 16.924 kb on + strand at 16.925 kb on - strand at 17.079 kb on + strand, within PS417_00075 at 17.080 kb on - strand, within PS417_00075 at 17.165 kb on - strand, within PS417_00075 at 17.165 kb on - strand, within PS417_00075 at 17.268 kb on - strand, within PS417_00075 at 17.701 kb on - strand, within PS417_00080 at 17.701 kb on - strand, within PS417_00080 at 17.767 kb on + strand, within PS417_00080 at 17.767 kb on + strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 15,309 - +1.2 15,375 + -3.1 15,412 + +0.4 15,412 + -1.4 15,412 + -1.2 15,421 + +1.7 15,422 - +0.1 15,524 + PS417_00065 0.27 -1.4 15,767 + PS417_00065 0.70 -1.0 16,856 + -0.4 16,924 + -0.9 16,924 + -0.3 16,925 - +0.2 17,079 + PS417_00075 0.26 -0.7 17,080 - PS417_00075 0.26 -0.9 17,165 - PS417_00075 0.54 -1.5 17,165 - PS417_00075 0.54 +0.5 17,268 - PS417_00075 0.87 +0.4 17,701 - PS417_00080 0.27 -2.1 17,701 - PS417_00080 0.27 -1.3 17,767 + PS417_00080 0.32 -0.3 17,767 + PS417_00080 0.32 -0.4 17,768 - PS417_00080 0.32 -3.8 17,768 - PS417_00080 0.32 +1.8 17,768 - PS417_00080 0.32 +1.1 17,768 - PS417_00080 0.32 -0.8 17,768 - PS417_00080 0.32 -0.4
Or see this region's nucleotide sequence