Experiment: Doxycycline hyclate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06375 and PS417_06380 are separated by 108 nucleotides PS417_06380 and PS417_06385 are separated by 3 nucleotides PS417_06385 and PS417_06390 overlap by 1 nucleotides PS417_06390 and PS417_06395 are separated by 57 nucleotides PS417_06395 and PS417_06400 are separated by 13 nucleotides
PS417_06375: PS417_06375 - deoxycytidine triphosphate deaminase, at 1,414,024 to 1,414,590
_06375
PS417_06380: PS417_06380 - hypothetical protein, at 1,414,699 to 1,414,896
_06380
PS417_06385: PS417_06385 - DEAD/DEAH box helicase, at 1,414,900 to 1,415,550
_06385
PS417_06390: PS417_06390 - DEAD/DEAH box helicase, at 1,415,550 to 1,418,030
_06390
PS417_06395: PS417_06395 - amino acid transporter, at 1,418,088 to 1,418,852
_06395
PS417_06400: PS417_06400 - succinylglutamate desuccinylase, at 1,418,866 to 1,419,984
_06400
Position (kb)
1415
1416
1417
1418
1419 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1414.603 kb on - strand at 1414.619 kb on - strand at 1414.639 kb on - strand at 1414.639 kb on - strand at 1414.643 kb on - strand at 1414.648 kb on + strand at 1414.648 kb on + strand at 1414.649 kb on - strand at 1414.693 kb on + strand at 1414.699 kb on - strand at 1414.699 kb on - strand at 1414.937 kb on + strand at 1414.938 kb on - strand at 1415.041 kb on + strand, within PS417_06385 at 1415.041 kb on + strand, within PS417_06385 at 1415.042 kb on - strand, within PS417_06385 at 1415.065 kb on + strand, within PS417_06385 at 1415.072 kb on + strand, within PS417_06385 at 1415.176 kb on + strand, within PS417_06385 at 1415.176 kb on + strand, within PS417_06385 at 1415.176 kb on + strand, within PS417_06385 at 1415.177 kb on - strand, within PS417_06385 at 1415.177 kb on - strand, within PS417_06385 at 1415.177 kb on - strand, within PS417_06385 at 1415.177 kb on - strand, within PS417_06385 at 1415.177 kb on - strand, within PS417_06385 at 1415.177 kb on - strand, within PS417_06385 at 1415.724 kb on + strand at 1415.725 kb on - strand at 1415.725 kb on - strand at 1415.725 kb on - strand at 1415.725 kb on - strand at 1415.748 kb on - strand at 1415.809 kb on + strand, within PS417_06390 at 1415.809 kb on + strand, within PS417_06390 at 1415.809 kb on + strand, within PS417_06390 at 1416.158 kb on - strand, within PS417_06390 at 1416.318 kb on + strand at 1416.409 kb on + strand, within PS417_06390 at 1416.409 kb on + strand, within PS417_06390 at 1416.410 kb on - strand, within PS417_06390 at 1416.410 kb on - strand, within PS417_06390 at 1416.492 kb on - strand, within PS417_06390 at 1416.746 kb on - strand, within PS417_06390 at 1416.775 kb on - strand, within PS417_06390 at 1416.791 kb on - strand, within PS417_06390 at 1416.847 kb on + strand, within PS417_06390 at 1416.848 kb on - strand, within PS417_06390 at 1417.233 kb on - strand, within PS417_06390 at 1417.233 kb on - strand, within PS417_06390 at 1417.239 kb on - strand, within PS417_06390 at 1417.279 kb on - strand, within PS417_06390 at 1417.350 kb on - strand, within PS417_06390 at 1417.367 kb on + strand, within PS417_06390 at 1417.888 kb on + strand at 1417.888 kb on + strand at 1417.889 kb on - strand at 1417.889 kb on - strand at 1417.954 kb on - strand at 1418.459 kb on - strand, within PS417_06395 at 1418.543 kb on + strand, within PS417_06395 at 1418.543 kb on + strand, within PS417_06395 at 1418.544 kb on - strand, within PS417_06395 at 1418.848 kb on + strand at 1418.848 kb on + strand at 1418.848 kb on + strand at 1418.848 kb on + strand at 1418.878 kb on + strand at 1418.879 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.001 mg/ml remove 1,414,603 - -1.7 1,414,619 - -0.5 1,414,639 - +0.4 1,414,639 - -0.2 1,414,643 - -0.7 1,414,648 + +0.1 1,414,648 + -0.8 1,414,649 - -1.0 1,414,693 + -0.6 1,414,699 - +0.3 1,414,699 - -0.2 1,414,937 + -0.3 1,414,938 - -0.8 1,415,041 + PS417_06385 0.22 -1.9 1,415,041 + PS417_06385 0.22 -0.3 1,415,042 - PS417_06385 0.22 -1.7 1,415,065 + PS417_06385 0.25 +1.3 1,415,072 + PS417_06385 0.26 +1.1 1,415,176 + PS417_06385 0.42 -1.1 1,415,176 + PS417_06385 0.42 +0.6 1,415,176 + PS417_06385 0.42 -0.5 1,415,177 - PS417_06385 0.43 -1.0 1,415,177 - PS417_06385 0.43 -0.9 1,415,177 - PS417_06385 0.43 +0.5 1,415,177 - PS417_06385 0.43 +0.1 1,415,177 - PS417_06385 0.43 +0.3 1,415,177 - PS417_06385 0.43 -1.3 1,415,724 + -0.7 1,415,725 - -0.3 1,415,725 - +1.3 1,415,725 - -1.6 1,415,725 - +1.8 1,415,748 - -0.4 1,415,809 + PS417_06390 0.10 -1.6 1,415,809 + PS417_06390 0.10 -0.2 1,415,809 + PS417_06390 0.10 -1.2 1,416,158 - PS417_06390 0.25 -0.3 1,416,318 + +1.7 1,416,409 + PS417_06390 0.35 -0.7 1,416,409 + PS417_06390 0.35 -0.9 1,416,410 - PS417_06390 0.35 -0.2 1,416,410 - PS417_06390 0.35 -0.9 1,416,492 - PS417_06390 0.38 -0.2 1,416,746 - PS417_06390 0.48 +1.0 1,416,775 - PS417_06390 0.49 -0.6 1,416,791 - PS417_06390 0.50 -1.1 1,416,847 + PS417_06390 0.52 +0.4 1,416,848 - PS417_06390 0.52 -0.1 1,417,233 - PS417_06390 0.68 -2.2 1,417,233 - PS417_06390 0.68 +0.3 1,417,239 - PS417_06390 0.68 -0.3 1,417,279 - PS417_06390 0.70 -1.2 1,417,350 - PS417_06390 0.73 -1.7 1,417,367 + PS417_06390 0.73 +1.2 1,417,888 + -2.2 1,417,888 + -0.1 1,417,889 - -0.3 1,417,889 - -2.7 1,417,954 - -2.7 1,418,459 - PS417_06395 0.48 -0.2 1,418,543 + PS417_06395 0.59 +0.9 1,418,543 + PS417_06395 0.59 -0.3 1,418,544 - PS417_06395 0.60 -1.1 1,418,848 + -0.3 1,418,848 + -1.0 1,418,848 + +0.0 1,418,848 + +0.7 1,418,878 + -0.4 1,418,879 - +1.3
Or see this region's nucleotide sequence