Experiment: Polymyxin B sulfate 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24670 and PS417_24675 are separated by 228 nucleotides PS417_24675 and PS417_24680 are separated by 61 nucleotides PS417_24680 and PS417_24685 are separated by 130 nucleotides
PS417_24670: PS417_24670 - DNA polymerase III subunit delta, at 5,353,644 to 5,354,681
_24670
PS417_24675: PS417_24675 - lipoyl synthase, at 5,354,910 to 5,355,074
_24675
PS417_24680: PS417_24680 - murein transglycosylase, at 5,355,136 to 5,356,461
_24680
PS417_24685: PS417_24685 - peptidase S66, at 5,356,592 to 5,357,512
_24685
Position (kb)
5355
5356
5357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5354.808 kb on - strand at 5354.818 kb on - strand at 5354.829 kb on + strand at 5354.829 kb on + strand at 5354.829 kb on + strand at 5354.830 kb on - strand at 5354.830 kb on - strand at 5354.830 kb on - strand at 5354.830 kb on - strand at 5354.830 kb on - strand at 5355.039 kb on - strand, within PS417_24675 at 5355.041 kb on + strand, within PS417_24675 at 5355.042 kb on - strand, within PS417_24675 at 5355.042 kb on - strand, within PS417_24675 at 5355.058 kb on - strand at 5355.058 kb on - strand at 5355.098 kb on + strand at 5355.098 kb on + strand at 5355.098 kb on + strand at 5355.099 kb on - strand at 5355.099 kb on - strand at 5355.099 kb on - strand at 5355.119 kb on - strand at 5355.119 kb on - strand at 5355.491 kb on - strand, within PS417_24680 at 5355.491 kb on - strand, within PS417_24680 at 5355.521 kb on + strand, within PS417_24680 at 5355.522 kb on - strand, within PS417_24680 at 5355.522 kb on - strand, within PS417_24680 at 5355.526 kb on - strand, within PS417_24680 at 5355.550 kb on + strand, within PS417_24680 at 5355.654 kb on + strand, within PS417_24680 at 5355.655 kb on - strand, within PS417_24680 at 5355.688 kb on + strand, within PS417_24680 at 5355.689 kb on - strand, within PS417_24680 at 5355.829 kb on + strand, within PS417_24680 at 5355.829 kb on + strand, within PS417_24680 at 5355.829 kb on + strand, within PS417_24680 at 5355.829 kb on + strand, within PS417_24680 at 5355.830 kb on - strand, within PS417_24680 at 5355.830 kb on - strand, within PS417_24680 at 5355.830 kb on - strand, within PS417_24680 at 5355.830 kb on - strand, within PS417_24680 at 5355.830 kb on - strand, within PS417_24680 at 5356.174 kb on + strand, within PS417_24680 at 5356.174 kb on + strand, within PS417_24680 at 5356.174 kb on + strand, within PS417_24680 at 5356.175 kb on - strand, within PS417_24680 at 5356.175 kb on - strand, within PS417_24680 at 5356.294 kb on - strand, within PS417_24680 at 5356.328 kb on - strand, within PS417_24680 at 5356.469 kb on + strand at 5356.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.006 mg/ml remove 5,354,808 - +0.0 5,354,818 - -0.7 5,354,829 + -0.5 5,354,829 + -0.5 5,354,829 + +0.1 5,354,830 - -0.2 5,354,830 - -0.4 5,354,830 - -0.3 5,354,830 - -0.3 5,354,830 - -0.0 5,355,039 - PS417_24675 0.78 +1.1 5,355,041 + PS417_24675 0.79 +0.9 5,355,042 - PS417_24675 0.80 +2.2 5,355,042 - PS417_24675 0.80 +0.4 5,355,058 - +0.2 5,355,058 - -1.3 5,355,098 + +0.4 5,355,098 + -1.2 5,355,098 + -0.3 5,355,099 - -0.8 5,355,099 - -0.9 5,355,099 - +0.1 5,355,119 - -0.1 5,355,119 - +1.5 5,355,491 - PS417_24680 0.27 -0.7 5,355,491 - PS417_24680 0.27 -0.9 5,355,521 + PS417_24680 0.29 -0.5 5,355,522 - PS417_24680 0.29 -1.0 5,355,522 - PS417_24680 0.29 -0.8 5,355,526 - PS417_24680 0.29 -0.9 5,355,550 + PS417_24680 0.31 -0.3 5,355,654 + PS417_24680 0.39 -0.6 5,355,655 - PS417_24680 0.39 -0.8 5,355,688 + PS417_24680 0.42 -0.1 5,355,689 - PS417_24680 0.42 +0.8 5,355,829 + PS417_24680 0.52 -0.6 5,355,829 + PS417_24680 0.52 -0.7 5,355,829 + PS417_24680 0.52 -0.7 5,355,829 + PS417_24680 0.52 -1.2 5,355,830 - PS417_24680 0.52 -1.4 5,355,830 - PS417_24680 0.52 -1.8 5,355,830 - PS417_24680 0.52 -0.5 5,355,830 - PS417_24680 0.52 -0.9 5,355,830 - PS417_24680 0.52 -0.5 5,356,174 + PS417_24680 0.78 -0.7 5,356,174 + PS417_24680 0.78 -0.6 5,356,174 + PS417_24680 0.78 -0.8 5,356,175 - PS417_24680 0.78 -0.1 5,356,175 - PS417_24680 0.78 -0.6 5,356,294 - PS417_24680 0.87 -0.2 5,356,328 - PS417_24680 0.90 -0.7 5,356,469 + +0.4 5,356,470 - -0.7
Or see this region's nucleotide sequence