Experiment: Polymyxin B sulfate 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15075 and PS417_15080 are separated by 483 nucleotides PS417_15080 and PS417_15085 are separated by 146 nucleotides PS417_15085 and PS417_15090 are separated by 5 nucleotides PS417_15090 and PS417_15095 overlap by 4 nucleotides
PS417_15075: PS417_15075 - NAD-glutamate dehydrogenase, at 3,293,439 to 3,298,307
_15075
PS417_15080: PS417_15080 - hypothetical protein, at 3,298,791 to 3,299,144
_15080
PS417_15085: PS417_15085 - ATPase AAA, at 3,299,291 to 3,300,250
_15085
PS417_15090: PS417_15090 - hypothetical protein, at 3,300,256 to 3,301,188
_15090
PS417_15095: PS417_15095 - hypothetical protein, at 3,301,185 to 3,301,679
_15095
Position (kb)
3299
3300
3301 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3298.332 kb on + strand at 3298.452 kb on + strand at 3298.452 kb on + strand at 3298.453 kb on - strand at 3298.453 kb on - strand at 3299.130 kb on + strand at 3299.228 kb on - strand at 3299.335 kb on + strand at 3299.335 kb on + strand at 3299.336 kb on - strand at 3299.402 kb on - strand, within PS417_15085 at 3299.537 kb on + strand, within PS417_15085 at 3299.690 kb on + strand, within PS417_15085 at 3299.691 kb on - strand, within PS417_15085 at 3299.753 kb on + strand, within PS417_15085 at 3299.833 kb on + strand, within PS417_15085 at 3299.939 kb on + strand, within PS417_15085 at 3299.940 kb on - strand, within PS417_15085 at 3299.940 kb on - strand, within PS417_15085 at 3299.963 kb on + strand, within PS417_15085 at 3299.963 kb on + strand, within PS417_15085 at 3300.425 kb on - strand, within PS417_15090 at 3301.039 kb on + strand, within PS417_15090 at 3301.039 kb on + strand, within PS417_15090
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.006 mg/ml remove 3,298,332 + +0.7 3,298,452 + +0.9 3,298,452 + -0.1 3,298,453 - +0.6 3,298,453 - -0.6 3,299,130 + +0.1 3,299,228 - -2.5 3,299,335 + -1.9 3,299,335 + -1.7 3,299,336 - -1.7 3,299,402 - PS417_15085 0.12 -2.9 3,299,537 + PS417_15085 0.26 -2.7 3,299,690 + PS417_15085 0.42 +0.3 3,299,691 - PS417_15085 0.42 -1.4 3,299,753 + PS417_15085 0.48 -2.5 3,299,833 + PS417_15085 0.56 +0.6 3,299,939 + PS417_15085 0.68 -1.8 3,299,940 - PS417_15085 0.68 -1.3 3,299,940 - PS417_15085 0.68 -2.0 3,299,963 + PS417_15085 0.70 -0.7 3,299,963 + PS417_15085 0.70 -3.4 3,300,425 - PS417_15090 0.18 -2.0 3,301,039 + PS417_15090 0.84 -1.3 3,301,039 + PS417_15090 0.84 -2.6
Or see this region's nucleotide sequence