Experiment: Polymyxin B sulfate 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08175 and PS417_08180 are separated by 6 nucleotides PS417_08180 and PS417_08185 are separated by 128 nucleotides
PS417_08175: PS417_08175 - UDP-N-acetylglucosamine 2-epimerase, at 1,789,873 to 1,791,003
_08175
PS417_08180: PS417_08180 - glycosyl transferase, at 1,791,010 to 1,792,269
_08180
PS417_08185: PS417_08185 - glycosyl transferase, at 1,792,398 to 1,793,411
_08185
Position (kb)
1791
1792
1793 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1791.055 kb on + strand at 1791.056 kb on - strand at 1791.277 kb on + strand, within PS417_08180 at 1791.277 kb on + strand, within PS417_08180 at 1791.278 kb on - strand, within PS417_08180 at 1791.278 kb on - strand, within PS417_08180 at 1791.287 kb on - strand, within PS417_08180 at 1791.316 kb on + strand, within PS417_08180 at 1791.317 kb on - strand, within PS417_08180 at 1791.526 kb on + strand, within PS417_08180 at 1791.541 kb on + strand, within PS417_08180 at 1791.541 kb on + strand at 1791.551 kb on + strand, within PS417_08180 at 1791.551 kb on + strand, within PS417_08180 at 1791.552 kb on - strand, within PS417_08180 at 1791.586 kb on + strand, within PS417_08180 at 1791.587 kb on - strand, within PS417_08180 at 1791.587 kb on - strand, within PS417_08180 at 1791.591 kb on + strand, within PS417_08180 at 1791.591 kb on + strand, within PS417_08180 at 1791.591 kb on + strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.592 kb on - strand, within PS417_08180 at 1791.622 kb on + strand, within PS417_08180 at 1791.705 kb on + strand, within PS417_08180 at 1791.705 kb on + strand, within PS417_08180 at 1791.705 kb on + strand at 1791.705 kb on + strand, within PS417_08180 at 1791.984 kb on + strand, within PS417_08180 at 1791.998 kb on - strand, within PS417_08180 at 1792.113 kb on + strand, within PS417_08180 at 1792.122 kb on + strand, within PS417_08180 at 1792.123 kb on - strand, within PS417_08180 at 1792.123 kb on - strand, within PS417_08180 at 1792.183 kb on + strand at 1792.183 kb on + strand at 1792.184 kb on - strand at 1792.279 kb on + strand at 1792.279 kb on + strand at 1792.280 kb on - strand at 1792.307 kb on - strand at 1792.307 kb on - strand at 1792.356 kb on + strand at 1792.357 kb on - strand at 1792.395 kb on + strand at 1792.396 kb on - strand at 1792.396 kb on - strand at 1792.396 kb on - strand at 1792.445 kb on - strand at 1792.468 kb on - strand at 1792.586 kb on - strand, within PS417_08185 at 1792.682 kb on + strand, within PS417_08185 at 1792.808 kb on - strand, within PS417_08185 at 1792.808 kb on - strand at 1792.822 kb on - strand, within PS417_08185 at 1792.822 kb on - strand, within PS417_08185 at 1792.848 kb on - strand, within PS417_08185 at 1792.921 kb on - strand at 1792.921 kb on - strand, within PS417_08185 at 1792.921 kb on - strand, within PS417_08185 at 1792.991 kb on - strand, within PS417_08185 at 1793.132 kb on - strand, within PS417_08185 at 1793.159 kb on - strand, within PS417_08185 at 1793.191 kb on - strand, within PS417_08185
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.006 mg/ml remove 1,791,055 + -1.4 1,791,056 - -2.3 1,791,277 + PS417_08180 0.21 -2.1 1,791,277 + PS417_08180 0.21 -2.6 1,791,278 - PS417_08180 0.21 -2.6 1,791,278 - PS417_08180 0.21 +0.7 1,791,287 - PS417_08180 0.22 -0.3 1,791,316 + PS417_08180 0.24 -1.1 1,791,317 - PS417_08180 0.24 -2.4 1,791,526 + PS417_08180 0.41 -2.3 1,791,541 + PS417_08180 0.42 -3.5 1,791,541 + +0.7 1,791,551 + PS417_08180 0.43 -2.4 1,791,551 + PS417_08180 0.43 -2.4 1,791,552 - PS417_08180 0.43 -3.5 1,791,586 + PS417_08180 0.46 -1.8 1,791,587 - PS417_08180 0.46 -1.3 1,791,587 - PS417_08180 0.46 -1.7 1,791,591 + PS417_08180 0.46 -1.4 1,791,591 + PS417_08180 0.46 -1.6 1,791,591 + PS417_08180 0.46 -1.8 1,791,592 - PS417_08180 0.46 -1.6 1,791,592 - PS417_08180 0.46 -3.3 1,791,592 - PS417_08180 0.46 -1.3 1,791,592 - PS417_08180 0.46 -1.6 1,791,592 - PS417_08180 0.46 -1.2 1,791,592 - PS417_08180 0.46 -1.6 1,791,592 - PS417_08180 0.46 -1.7 1,791,622 + PS417_08180 0.49 -1.3 1,791,705 + PS417_08180 0.55 +0.1 1,791,705 + PS417_08180 0.55 -0.3 1,791,705 + +1.7 1,791,705 + PS417_08180 0.55 -2.1 1,791,984 + PS417_08180 0.77 -1.6 1,791,998 - PS417_08180 0.78 -2.0 1,792,113 + PS417_08180 0.88 -4.0 1,792,122 + PS417_08180 0.88 -2.2 1,792,123 - PS417_08180 0.88 -1.8 1,792,123 - PS417_08180 0.88 -0.8 1,792,183 + -2.4 1,792,183 + -1.6 1,792,184 - -2.6 1,792,279 + +2.7 1,792,279 + -1.6 1,792,280 - -0.8 1,792,307 - -2.3 1,792,307 - -0.7 1,792,356 + +0.2 1,792,357 - -2.3 1,792,395 + -1.3 1,792,396 - -2.0 1,792,396 - -2.0 1,792,396 - -1.6 1,792,445 - -3.0 1,792,468 - -3.1 1,792,586 - PS417_08185 0.19 -2.8 1,792,682 + PS417_08185 0.28 -1.9 1,792,808 - PS417_08185 0.40 -1.1 1,792,808 - -0.3 1,792,822 - PS417_08185 0.42 -2.1 1,792,822 - PS417_08185 0.42 -2.2 1,792,848 - PS417_08185 0.44 -1.6 1,792,921 - -0.8 1,792,921 - PS417_08185 0.52 -2.4 1,792,921 - PS417_08185 0.52 -0.3 1,792,991 - PS417_08185 0.58 -3.2 1,793,132 - PS417_08185 0.72 -2.1 1,793,159 - PS417_08185 0.75 -2.6 1,793,191 - PS417_08185 0.78 -1.3
Or see this region's nucleotide sequence