Strain Fitness in Pseudomonas simiae WCS417 around PS417_05505

Experiment: Polymyxin B sulfate 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_05495 and PS417_05500 are separated by 228 nucleotidesPS417_05500 and PS417_05505 overlap by 8 nucleotidesPS417_05505 and PS417_05510 overlap by 4 nucleotidesPS417_05510 and PS417_05515 are separated by 31 nucleotides PS417_05495: PS417_05495 - structural protein MipA, at 1,236,255 to 1,237,025 _05495 PS417_05500: PS417_05500 - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, at 1,237,254 to 1,237,955 _05500 PS417_05505: PS417_05505 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 1,237,948 to 1,239,342 _05505 PS417_05510: PS417_05510 - alpha/beta hydrolase, at 1,239,339 to 1,240,289 _05510 PS417_05515: PS417_05515 - hypothetical protein, at 1,240,321 to 1,241,445 _05515 Position (kb) 1237 1238 1239 1240Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1237.047 kb on + strandat 1237.047 kb on + strandat 1237.047 kb on + strandat 1237.111 kb on - strandat 1237.205 kb on + strandat 1237.245 kb on + strandat 1237.246 kb on - strandat 1237.452 kb on + strand, within PS417_05500at 1237.582 kb on - strand, within PS417_05500at 1238.008 kb on + strandat 1238.008 kb on + strandat 1238.009 kb on - strandat 1238.035 kb on + strandat 1238.036 kb on - strandat 1238.036 kb on - strandat 1238.077 kb on + strandat 1238.077 kb on + strandat 1238.104 kb on + strand, within PS417_05505at 1238.104 kb on + strand, within PS417_05505at 1238.126 kb on - strand, within PS417_05505at 1238.215 kb on + strand, within PS417_05505at 1238.216 kb on - strand, within PS417_05505at 1238.427 kb on + strand, within PS417_05505at 1238.428 kb on - strand, within PS417_05505at 1238.584 kb on + strand, within PS417_05505at 1238.623 kb on + strand, within PS417_05505at 1238.624 kb on - strand, within PS417_05505at 1238.636 kb on - strand, within PS417_05505at 1238.666 kb on - strandat 1238.761 kb on + strand, within PS417_05505at 1239.155 kb on - strand, within PS417_05505at 1239.155 kb on - strand, within PS417_05505at 1239.155 kb on - strand, within PS417_05505at 1239.155 kb on - strand, within PS417_05505at 1239.232 kb on + strandat 1239.350 kb on - strandat 1239.495 kb on + strand, within PS417_05510at 1239.496 kb on - strand, within PS417_05510at 1239.657 kb on + strand, within PS417_05510at 1239.657 kb on + strand, within PS417_05510at 1239.657 kb on + strand, within PS417_05510at 1239.657 kb on + strand, within PS417_05510at 1239.657 kb on + strand, within PS417_05510at 1239.658 kb on - strand, within PS417_05510at 1239.703 kb on - strand, within PS417_05510at 1239.720 kb on + strand, within PS417_05510at 1239.882 kb on - strand, within PS417_05510at 1240.035 kb on + strand, within PS417_05510

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.006 mg/ml
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1,237,047 + -0.8
1,237,047 + -1.3
1,237,047 + +0.9
1,237,111 - -2.0
1,237,205 + -1.7
1,237,245 + -1.8
1,237,246 - -1.7
1,237,452 + PS417_05500 0.28 -3.2
1,237,582 - PS417_05500 0.47 -1.0
1,238,008 + -1.2
1,238,008 + -0.1
1,238,009 - -1.4
1,238,035 + -3.6
1,238,036 - -1.8
1,238,036 - -2.5
1,238,077 + -2.8
1,238,077 + -1.9
1,238,104 + PS417_05505 0.11 -3.2
1,238,104 + PS417_05505 0.11 -3.8
1,238,126 - PS417_05505 0.13 -1.2
1,238,215 + PS417_05505 0.19 -3.2
1,238,216 - PS417_05505 0.19 -1.1
1,238,427 + PS417_05505 0.34 -3.6
1,238,428 - PS417_05505 0.34 -4.4
1,238,584 + PS417_05505 0.46 -2.3
1,238,623 + PS417_05505 0.48 -3.8
1,238,624 - PS417_05505 0.48 -3.0
1,238,636 - PS417_05505 0.49 -1.4
1,238,666 - -1.1
1,238,761 + PS417_05505 0.58 -1.4
1,239,155 - PS417_05505 0.87 -2.2
1,239,155 - PS417_05505 0.87 -3.1
1,239,155 - PS417_05505 0.87 -3.1
1,239,155 - PS417_05505 0.87 -1.7
1,239,232 + -3.2
1,239,350 - -2.5
1,239,495 + PS417_05510 0.16 -3.8
1,239,496 - PS417_05510 0.17 -3.0
1,239,657 + PS417_05510 0.33 -2.1
1,239,657 + PS417_05510 0.33 -2.7
1,239,657 + PS417_05510 0.33 -2.3
1,239,657 + PS417_05510 0.33 -3.1
1,239,657 + PS417_05510 0.33 -3.0
1,239,658 - PS417_05510 0.34 -1.1
1,239,703 - PS417_05510 0.38 -2.0
1,239,720 + PS417_05510 0.40 -0.9
1,239,882 - PS417_05510 0.57 -0.1
1,240,035 + PS417_05510 0.73 -2.8

Or see this region's nucleotide sequence