Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS03020

Experiment: NAG (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS03015 and PFLU_RS03020 are separated by 81 nucleotidesPFLU_RS03020 and PFLU_RS03025 overlap by 4 nucleotides PFLU_RS03015: PFLU_RS03015 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH, at 695,158 to 696,765 _RS03015 PFLU_RS03020: PFLU_RS03020 - DNA-binding transcriptional regulator Fis, at 696,847 to 697,167 _RS03020 PFLU_RS03025: PFLU_RS03025 - tRNA dihydrouridine synthase DusB, at 697,164 to 698,177 _RS03025 Position (kb) 696 697 698Strain fitness (log2 ratio) -1 0 1 2at 695.979 kb on - strand, within PFLU_RS03015at 696.047 kb on + strand, within PFLU_RS03015at 696.047 kb on + strand, within PFLU_RS03015at 696.048 kb on - strand, within PFLU_RS03015at 696.185 kb on + strand, within PFLU_RS03015at 696.185 kb on + strand, within PFLU_RS03015at 696.185 kb on + strand, within PFLU_RS03015at 696.186 kb on - strand, within PFLU_RS03015at 696.186 kb on - strand, within PFLU_RS03015at 696.186 kb on - strand, within PFLU_RS03015at 696.816 kb on - strandat 696.848 kb on + strandat 697.207 kb on + strandat 697.208 kb on - strandat 697.208 kb on - strandat 697.253 kb on + strandat 697.313 kb on - strand, within PFLU_RS03025at 697.342 kb on - strand, within PFLU_RS03025at 697.342 kb on - strand, within PFLU_RS03025at 697.360 kb on + strand, within PFLU_RS03025at 697.361 kb on - strand, within PFLU_RS03025at 697.361 kb on - strand, within PFLU_RS03025at 697.361 kb on - strand, within PFLU_RS03025at 697.389 kb on + strand, within PFLU_RS03025at 697.390 kb on - strand, within PFLU_RS03025at 697.398 kb on - strand, within PFLU_RS03025at 697.432 kb on - strand, within PFLU_RS03025at 697.432 kb on - strand, within PFLU_RS03025at 697.622 kb on - strand, within PFLU_RS03025at 697.639 kb on + strand, within PFLU_RS03025at 697.680 kb on - strand, within PFLU_RS03025at 698.062 kb on - strand, within PFLU_RS03025at 698.062 kb on - strand, within PFLU_RS03025at 698.062 kb on - strand, within PFLU_RS03025at 698.062 kb on - strand, within PFLU_RS03025at 698.153 kb on - strandat 698.153 kb on - strandat 698.153 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NAG (C)
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695,979 - PFLU_RS03015 0.51 +0.3
696,047 + PFLU_RS03015 0.55 -0.3
696,047 + PFLU_RS03015 0.55 -1.6
696,048 - PFLU_RS03015 0.55 -0.9
696,185 + PFLU_RS03015 0.64 -0.3
696,185 + PFLU_RS03015 0.64 +0.7
696,185 + PFLU_RS03015 0.64 -0.3
696,186 - PFLU_RS03015 0.64 -1.2
696,186 - PFLU_RS03015 0.64 -1.0
696,186 - PFLU_RS03015 0.64 -0.6
696,816 - +1.6
696,848 + -1.1
697,207 + +0.8
697,208 - +0.1
697,208 - +0.3
697,253 + +0.3
697,313 - PFLU_RS03025 0.15 +0.5
697,342 - PFLU_RS03025 0.18 +0.5
697,342 - PFLU_RS03025 0.18 -0.0
697,360 + PFLU_RS03025 0.19 -0.1
697,361 - PFLU_RS03025 0.19 -0.7
697,361 - PFLU_RS03025 0.19 +1.8
697,361 - PFLU_RS03025 0.19 -0.5
697,389 + PFLU_RS03025 0.22 +0.7
697,390 - PFLU_RS03025 0.22 +0.9
697,398 - PFLU_RS03025 0.23 -0.4
697,432 - PFLU_RS03025 0.26 +0.1
697,432 - PFLU_RS03025 0.26 +0.8
697,622 - PFLU_RS03025 0.45 +0.9
697,639 + PFLU_RS03025 0.47 -0.5
697,680 - PFLU_RS03025 0.51 +0.6
698,062 - PFLU_RS03025 0.89 +1.1
698,062 - PFLU_RS03025 0.89 +0.1
698,062 - PFLU_RS03025 0.89 -0.8
698,062 - PFLU_RS03025 0.89 -0.8
698,153 - +0.0
698,153 - +1.4
698,153 - -0.1

Or see this region's nucleotide sequence