Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydbK and ydbJ are separated by 273 nucleotides ydbJ and hslJ overlap by 4 nucleotides hslJ and ldhA are separated by 110 nucleotides
b1378: ydbK - fused predicted pyruvate-flavodoxin oxidoreductase: conserved protein/conserved protein/FeS binding protein (NCBI), at 1,435,284 to 1,438,808
ydbK
b4529: ydbJ - hypothetical protein (NCBI), at 1,439,082 to 1,439,348
ydbJ
b1379: hslJ - heat-inducible protein (NCBI), at 1,439,345 to 1,439,767
hslJ
b1380: ldhA - D-lactate dehydrogenase (NCBI), at 1,439,878 to 1,440,867
ldhA
Position (kb)
1439
1440 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1438.460 kb on + strand at 1438.501 kb on + strand at 1438.540 kb on - strand at 1438.709 kb on - strand at 1438.815 kb on + strand at 1438.823 kb on - strand at 1438.926 kb on - strand at 1438.926 kb on - strand at 1438.997 kb on + strand at 1438.998 kb on - strand at 1439.084 kb on - strand at 1439.084 kb on - strand at 1439.137 kb on - strand, within ydbJ at 1439.365 kb on + strand at 1439.401 kb on - strand, within hslJ at 1439.561 kb on - strand, within hslJ at 1439.561 kb on - strand, within hslJ at 1439.591 kb on + strand, within hslJ at 1439.591 kb on + strand, within hslJ at 1439.654 kb on + strand, within hslJ at 1439.836 kb on - strand at 1439.836 kb on - strand at 1439.865 kb on - strand at 1439.929 kb on + strand at 1439.995 kb on - strand, within ldhA at 1440.256 kb on + strand, within ldhA
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 1,438,460 + +0.9 1,438,501 + -2.9 1,438,540 - +2.1 1,438,709 - +0.5 1,438,815 + +1.0 1,438,823 - -1.0 1,438,926 - +4.7 1,438,926 - -1.7 1,438,997 + +1.3 1,438,998 - +1.8 1,439,084 - +0.7 1,439,084 - +1.5 1,439,137 - ydbJ b4529 0.21 -2.8 1,439,365 + -0.1 1,439,401 - hslJ b1379 0.13 +1.1 1,439,561 - hslJ b1379 0.51 +2.3 1,439,561 - hslJ b1379 0.51 -0.2 1,439,591 + hslJ b1379 0.58 -1.6 1,439,591 + hslJ b1379 0.58 -2.1 1,439,654 + hslJ b1379 0.73 +1.0 1,439,836 - -1.8 1,439,836 - +2.0 1,439,865 - +2.0 1,439,929 + +1.4 1,439,995 - ldhA b1380 0.12 -2.4 1,440,256 + ldhA b1380 0.38 -1.7
Or see this region's nucleotide sequence