Strain Fitness in Escherichia coli BW25113 around b4071

Experiment: octanol 0.08 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrfA and nrfB are separated by 44 nucleotidesnrfB and nrfC overlap by 4 nucleotidesnrfC and nrfD overlap by 4 nucleotides b4070: nrfA - nitrite reductase, formate-dependent, cytochrome (NCBI), at 4,285,787 to 4,287,223 nrfA b4071: nrfB - formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS), at 4,287,268 to 4,287,834 nrfB b4072: nrfC - formate-dependent nitrite reductase, 4Fe4S subunit (NCBI), at 4,287,831 to 4,288,502 nrfC b4073: nrfD - formate-dependent nitrite reductase, membrane subunit (NCBI), at 4,288,499 to 4,289,455 nrfD Position (kb) 4287 4288Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4286.340 kb on - strand, within nrfAat 4286.419 kb on + strand, within nrfAat 4286.419 kb on + strand, within nrfAat 4286.427 kb on - strand, within nrfAat 4286.427 kb on - strand, within nrfAat 4286.526 kb on + strand, within nrfAat 4286.526 kb on + strand, within nrfAat 4286.590 kb on + strand, within nrfAat 4286.629 kb on - strand, within nrfAat 4286.629 kb on - strand, within nrfAat 4286.674 kb on + strand, within nrfAat 4286.682 kb on - strand, within nrfAat 4286.846 kb on + strand, within nrfAat 4286.954 kb on + strand, within nrfAat 4286.962 kb on - strand, within nrfAat 4286.965 kb on - strand, within nrfAat 4286.965 kb on - strand, within nrfAat 4287.004 kb on + strand, within nrfAat 4287.058 kb on - strand, within nrfAat 4287.119 kb on + strandat 4287.149 kb on + strandat 4287.223 kb on + strandat 4287.250 kb on + strandat 4287.250 kb on + strandat 4287.250 kb on + strandat 4287.258 kb on - strandat 4287.262 kb on + strandat 4287.301 kb on + strandat 4287.301 kb on + strandat 4287.308 kb on + strandat 4287.313 kb on + strandat 4287.313 kb on + strandat 4287.328 kb on - strand, within nrfBat 4287.534 kb on - strand, within nrfBat 4287.560 kb on + strand, within nrfBat 4287.560 kb on + strand, within nrfBat 4287.582 kb on - strand, within nrfBat 4287.591 kb on + strand, within nrfBat 4287.619 kb on - strand, within nrfBat 4287.718 kb on + strand, within nrfBat 4287.823 kb on - strandat 4287.823 kb on - strandat 4287.836 kb on - strandat 4287.836 kb on - strandat 4287.905 kb on - strand, within nrfCat 4287.970 kb on - strand, within nrfCat 4287.981 kb on - strand, within nrfCat 4287.985 kb on + strand, within nrfCat 4288.125 kb on - strand, within nrfCat 4288.153 kb on - strand, within nrfCat 4288.182 kb on + strand, within nrfCat 4288.182 kb on + strand, within nrfCat 4288.212 kb on + strand, within nrfCat 4288.212 kb on + strand, within nrfCat 4288.220 kb on - strand, within nrfCat 4288.322 kb on + strand, within nrfCat 4288.458 kb on + strandat 4288.465 kb on + strandat 4288.465 kb on + strandat 4288.465 kb on + strandat 4288.465 kb on + strandat 4288.543 kb on - strandat 4288.617 kb on - strand, within nrfDat 4288.617 kb on - strand, within nrfDat 4288.650 kb on - strand, within nrfDat 4288.650 kb on - strand, within nrfDat 4288.655 kb on - strand, within nrfDat 4288.732 kb on + strand, within nrfDat 4288.740 kb on + strand, within nrfDat 4288.804 kb on + strand, within nrfDat 4288.804 kb on + strand, within nrfDat 4288.807 kb on + strand, within nrfDat 4288.825 kb on - strand, within nrfDat 4288.825 kb on - strand, within nrfD

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Per-strain Table

Position Strand Gene LocusTag Fraction octanol 0.08 vol%
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4,286,340 - nrfA b4070 0.38 +3.3
4,286,419 + nrfA b4070 0.44 +0.7
4,286,419 + nrfA b4070 0.44 -1.1
4,286,427 - nrfA b4070 0.45 +1.3
4,286,427 - nrfA b4070 0.45 -0.8
4,286,526 + nrfA b4070 0.51 -0.4
4,286,526 + nrfA b4070 0.51 +2.1
4,286,590 + nrfA b4070 0.56 +0.6
4,286,629 - nrfA b4070 0.59 +1.9
4,286,629 - nrfA b4070 0.59 +1.1
4,286,674 + nrfA b4070 0.62 -1.1
4,286,682 - nrfA b4070 0.62 +0.5
4,286,846 + nrfA b4070 0.74 +1.7
4,286,954 + nrfA b4070 0.81 -0.9
4,286,962 - nrfA b4070 0.82 -1.7
4,286,965 - nrfA b4070 0.82 -3.0
4,286,965 - nrfA b4070 0.82 -2.4
4,287,004 + nrfA b4070 0.85 +2.3
4,287,058 - nrfA b4070 0.88 -4.0
4,287,119 + +1.2
4,287,149 + +1.4
4,287,223 + +0.5
4,287,250 + -0.5
4,287,250 + -1.5
4,287,250 + -1.9
4,287,258 - -2.9
4,287,262 + -1.1
4,287,301 + +0.4
4,287,301 + -2.9
4,287,308 + +0.3
4,287,313 + +1.6
4,287,313 + +3.0
4,287,328 - nrfB b4071 0.11 -0.4
4,287,534 - nrfB b4071 0.47 -0.6
4,287,560 + nrfB b4071 0.51 +1.2
4,287,560 + nrfB b4071 0.51 -1.4
4,287,582 - nrfB b4071 0.55 -1.1
4,287,591 + nrfB b4071 0.57 +1.4
4,287,619 - nrfB b4071 0.62 -0.7
4,287,718 + nrfB b4071 0.79 +0.1
4,287,823 - -1.9
4,287,823 - -0.1
4,287,836 - +3.2
4,287,836 - +2.5
4,287,905 - nrfC b4072 0.11 +0.6
4,287,970 - nrfC b4072 0.21 -1.3
4,287,981 - nrfC b4072 0.22 -0.9
4,287,985 + nrfC b4072 0.23 +0.5
4,288,125 - nrfC b4072 0.44 +1.4
4,288,153 - nrfC b4072 0.48 +0.7
4,288,182 + nrfC b4072 0.52 -2.0
4,288,182 + nrfC b4072 0.52 +1.7
4,288,212 + nrfC b4072 0.57 +1.1
4,288,212 + nrfC b4072 0.57 +1.5
4,288,220 - nrfC b4072 0.58 +1.1
4,288,322 + nrfC b4072 0.73 -0.4
4,288,458 + +1.4
4,288,465 + +3.3
4,288,465 + -1.7
4,288,465 + -1.8
4,288,465 + -2.0
4,288,543 - -1.1
4,288,617 - nrfD b4073 0.12 -0.2
4,288,617 - nrfD b4073 0.12 +3.3
4,288,650 - nrfD b4073 0.16 -4.4
4,288,650 - nrfD b4073 0.16 +1.8
4,288,655 - nrfD b4073 0.16 +1.5
4,288,732 + nrfD b4073 0.24 +2.6
4,288,740 + nrfD b4073 0.25 +1.6
4,288,804 + nrfD b4073 0.32 +1.7
4,288,804 + nrfD b4073 0.32 -0.7
4,288,807 + nrfD b4073 0.32 +0.4
4,288,825 - nrfD b4073 0.34 +1.4
4,288,825 - nrfD b4073 0.34 +3.1

Or see this region's nucleotide sequence