Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recJ and dsbC are separated by 5 nucleotides dsbC and xerD are separated by 24 nucleotides
b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128
recJ
b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844
dsbC
b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765
xerD
Position (kb)
3036
3037 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3035.135 kb on - strand, within recJ at 3035.270 kb on + strand, within recJ at 3035.433 kb on + strand, within recJ at 3035.454 kb on - strand, within recJ at 3035.489 kb on + strand, within recJ at 3035.680 kb on - strand, within recJ at 3035.751 kb on - strand, within recJ at 3035.751 kb on - strand, within recJ at 3035.754 kb on + strand, within recJ at 3035.754 kb on + strand, within recJ at 3035.757 kb on - strand, within recJ at 3035.762 kb on - strand, within recJ at 3035.768 kb on + strand, within recJ at 3035.768 kb on + strand, within recJ at 3035.776 kb on - strand, within recJ at 3035.789 kb on - strand, within recJ at 3035.789 kb on - strand, within recJ at 3035.802 kb on + strand, within recJ at 3035.802 kb on + strand, within recJ at 3035.807 kb on + strand at 3035.815 kb on - strand, within recJ at 3035.854 kb on - strand, within recJ at 3035.854 kb on - strand, within recJ at 3035.864 kb on - strand, within recJ at 3035.869 kb on - strand, within recJ at 3035.906 kb on - strand, within recJ at 3035.906 kb on - strand at 3035.960 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3036.046 kb on - strand at 3036.068 kb on - strand at 3036.229 kb on + strand, within dsbC at 3036.229 kb on - strand, within dsbC at 3036.268 kb on + strand, within dsbC at 3036.457 kb on - strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.613 kb on - strand, within dsbC at 3036.626 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.666 kb on + strand, within dsbC at 3036.668 kb on + strand, within dsbC at 3036.761 kb on + strand, within dsbC at 3037.372 kb on + strand, within xerD at 3037.773 kb on + strand at 3037.777 kb on + strand at 3037.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 3,035,135 - recJ b2892 0.43 -2.0 3,035,270 + recJ b2892 0.50 +1.1 3,035,433 + recJ b2892 0.60 -0.5 3,035,454 - recJ b2892 0.61 -2.1 3,035,489 + recJ b2892 0.63 +0.6 3,035,680 - recJ b2892 0.74 -1.6 3,035,751 - recJ b2892 0.78 -0.3 3,035,751 - recJ b2892 0.78 -0.6 3,035,754 + recJ b2892 0.78 +2.6 3,035,754 + recJ b2892 0.78 +1.5 3,035,757 - recJ b2892 0.79 -1.6 3,035,762 - recJ b2892 0.79 -1.2 3,035,768 + recJ b2892 0.79 -1.0 3,035,768 + recJ b2892 0.79 -2.1 3,035,776 - recJ b2892 0.80 -0.8 3,035,789 - recJ b2892 0.80 -1.4 3,035,789 - recJ b2892 0.80 -1.1 3,035,802 + recJ b2892 0.81 -0.5 3,035,802 + recJ b2892 0.81 -2.9 3,035,807 + +1.1 3,035,815 - recJ b2892 0.82 +0.4 3,035,854 - recJ b2892 0.84 -1.5 3,035,854 - recJ b2892 0.84 +1.5 3,035,864 - recJ b2892 0.85 -3.3 3,035,869 - recJ b2892 0.85 +0.3 3,035,906 - recJ b2892 0.87 -1.6 3,035,906 - +0.6 3,035,960 - +2.2 3,035,996 - -0.4 3,035,996 - -0.2 3,035,996 - +0.2 3,036,046 - -0.2 3,036,068 - +0.1 3,036,229 + dsbC b2893 0.13 -0.8 3,036,229 - dsbC b2893 0.13 -0.8 3,036,268 + dsbC b2893 0.19 -2.2 3,036,457 - dsbC b2893 0.45 -0.8 3,036,511 + dsbC b2893 0.53 +0.6 3,036,511 + dsbC b2893 0.53 -1.3 3,036,613 - dsbC b2893 0.67 -1.5 3,036,626 - dsbC b2893 0.69 -0.7 3,036,660 - dsbC b2893 0.74 -1.6 3,036,660 - dsbC b2893 0.74 -2.0 3,036,666 + dsbC b2893 0.75 +1.0 3,036,668 + dsbC b2893 0.75 -1.4 3,036,761 + dsbC b2893 0.88 -0.3 3,037,372 + xerD b2894 0.56 +1.6 3,037,773 + -1.2 3,037,777 + -1.7 3,037,777 + +3.4
Or see this region's nucleotide sequence