Strain Fitness in Escherichia coli BW25113 around b2685

Experiment: octanol 0.08 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygaH and mprA are separated by 90 nucleotidesmprA and emrA are separated by 126 nucleotidesemrA and emrB are separated by 16 nucleotides b2683: ygaH - predicted inner membrane protein (NCBI), at 2,808,366 to 2,808,701 ygaH b2684: mprA - DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI), at 2,808,792 to 2,809,322 mprA b2685: emrA - multidrug efflux system (NCBI), at 2,809,449 to 2,810,621 emrA b2686: emrB - multidrug efflux system protein (NCBI), at 2,810,638 to 2,812,176 emrB Position (kb) 2809 2810 2811Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 2808.515 kb on + strand, within ygaHat 2808.517 kb on - strand, within ygaHat 2808.533 kb on + strand, within ygaHat 2808.533 kb on + strand, within ygaHat 2808.534 kb on - strand, within ygaHat 2808.534 kb on - strand, within ygaHat 2808.560 kb on - strand, within ygaHat 2808.565 kb on - strandat 2808.645 kb on + strand, within ygaHat 2808.645 kb on + strand, within ygaHat 2808.645 kb on + strand, within ygaHat 2808.648 kb on - strand, within ygaHat 2808.655 kb on + strand, within ygaHat 2808.658 kb on - strand, within ygaHat 2808.660 kb on - strand, within ygaHat 2808.662 kb on + strand, within ygaHat 2808.662 kb on + strand, within ygaHat 2808.671 kb on - strandat 2808.876 kb on - strand, within mprAat 2809.004 kb on - strand, within mprAat 2809.100 kb on + strand, within mprAat 2809.100 kb on + strand, within mprAat 2809.107 kb on + strand, within mprAat 2809.200 kb on + strand, within mprAat 2809.287 kb on + strandat 2809.433 kb on - strandat 2809.497 kb on + strandat 2809.497 kb on + strandat 2809.546 kb on - strandat 2809.548 kb on - strandat 2809.748 kb on - strand, within emrAat 2809.748 kb on - strand, within emrAat 2809.806 kb on - strand, within emrAat 2809.931 kb on - strand, within emrAat 2810.041 kb on + strand, within emrAat 2810.097 kb on + strand, within emrAat 2810.097 kb on + strand, within emrAat 2810.097 kb on + strand, within emrAat 2810.100 kb on - strand, within emrAat 2810.100 kb on - strand, within emrAat 2810.103 kb on - strand, within emrAat 2810.127 kb on - strand, within emrAat 2810.147 kb on - strand, within emrAat 2810.204 kb on - strand, within emrAat 2810.320 kb on + strand, within emrAat 2810.339 kb on - strand, within emrAat 2810.417 kb on - strand, within emrAat 2810.443 kb on - strand, within emrAat 2810.480 kb on + strand, within emrAat 2810.482 kb on - strand, within emrAat 2810.482 kb on - strand, within emrAat 2810.503 kb on + strand, within emrAat 2810.533 kb on + strandat 2810.543 kb on + strandat 2810.543 kb on + strandat 2810.549 kb on - strandat 2810.621 kb on + strandat 2810.633 kb on + strandat 2810.716 kb on + strandat 2810.716 kb on + strandat 2810.724 kb on - strandat 2810.763 kb on - strandat 2810.764 kb on + strandat 2810.764 kb on + strandat 2810.921 kb on + strand, within emrBat 2811.019 kb on - strand, within emrBat 2811.019 kb on - strand, within emrBat 2811.034 kb on + strand, within emrBat 2811.034 kb on + strand, within emrBat 2811.151 kb on - strand, within emrBat 2811.196 kb on - strand, within emrBat 2811.196 kb on - strand, within emrBat 2811.310 kb on + strand, within emrBat 2811.431 kb on - strand, within emrBat 2811.436 kb on - strand, within emrBat 2811.505 kb on - strand, within emrBat 2811.507 kb on - strand, within emrBat 2811.507 kb on - strand, within emrBat 2811.528 kb on + strandat 2811.581 kb on - strand, within emrB

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Per-strain Table

Position Strand Gene LocusTag Fraction octanol 0.08 vol%
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2,808,515 + ygaH b2683 0.44 -4.5
2,808,517 - ygaH b2683 0.45 +0.4
2,808,533 + ygaH b2683 0.50 -2.5
2,808,533 + ygaH b2683 0.50 +0.0
2,808,534 - ygaH b2683 0.50 +1.8
2,808,534 - ygaH b2683 0.50 +1.2
2,808,560 - ygaH b2683 0.58 +1.6
2,808,565 - +5.2
2,808,645 + ygaH b2683 0.83 -2.9
2,808,645 + ygaH b2683 0.83 -4.6
2,808,645 + ygaH b2683 0.83 -1.8
2,808,648 - ygaH b2683 0.84 -1.5
2,808,655 + ygaH b2683 0.86 -1.5
2,808,658 - ygaH b2683 0.87 +1.8
2,808,660 - ygaH b2683 0.88 +1.1
2,808,662 + ygaH b2683 0.88 -2.2
2,808,662 + ygaH b2683 0.88 -1.2
2,808,671 - +1.6
2,808,876 - mprA b2684 0.16 +5.6
2,809,004 - mprA b2684 0.40 +4.3
2,809,100 + mprA b2684 0.58 +4.5
2,809,100 + mprA b2684 0.58 +3.6
2,809,107 + mprA b2684 0.59 +5.0
2,809,200 + mprA b2684 0.77 +4.7
2,809,287 + +3.6
2,809,433 - +3.0
2,809,497 + +0.8
2,809,497 + +2.0
2,809,546 - +2.4
2,809,548 - -0.7
2,809,748 - emrA b2685 0.25 -0.3
2,809,748 - emrA b2685 0.25 -1.0
2,809,806 - emrA b2685 0.30 +1.7
2,809,931 - emrA b2685 0.41 +2.8
2,810,041 + emrA b2685 0.50 +2.7
2,810,097 + emrA b2685 0.55 +1.2
2,810,097 + emrA b2685 0.55 -0.7
2,810,097 + emrA b2685 0.55 +3.6
2,810,100 - emrA b2685 0.55 -2.0
2,810,100 - emrA b2685 0.55 +2.6
2,810,103 - emrA b2685 0.56 -2.2
2,810,127 - emrA b2685 0.58 -0.1
2,810,147 - emrA b2685 0.60 -1.6
2,810,204 - emrA b2685 0.64 -0.7
2,810,320 + emrA b2685 0.74 -0.2
2,810,339 - emrA b2685 0.76 +0.5
2,810,417 - emrA b2685 0.83 +1.9
2,810,443 - emrA b2685 0.85 +0.5
2,810,480 + emrA b2685 0.88 -3.5
2,810,482 - emrA b2685 0.88 +1.7
2,810,482 - emrA b2685 0.88 -1.2
2,810,503 + emrA b2685 0.90 -2.6
2,810,533 + +1.7
2,810,543 + +3.5
2,810,543 + -1.6
2,810,549 - -2.9
2,810,621 + -0.7
2,810,633 + -1.2
2,810,716 + -0.7
2,810,716 + +1.3
2,810,724 - -0.7
2,810,763 - -3.2
2,810,764 + +1.7
2,810,764 + +1.3
2,810,921 + emrB b2686 0.18 -1.2
2,811,019 - emrB b2686 0.25 -2.2
2,811,019 - emrB b2686 0.25 -2.2
2,811,034 + emrB b2686 0.26 +0.1
2,811,034 + emrB b2686 0.26 +0.8
2,811,151 - emrB b2686 0.33 +3.4
2,811,196 - emrB b2686 0.36 -1.1
2,811,196 - emrB b2686 0.36 +3.2
2,811,310 + emrB b2686 0.44 -0.4
2,811,431 - emrB b2686 0.52 -3.4
2,811,436 - emrB b2686 0.52 -1.5
2,811,505 - emrB b2686 0.56 +1.4
2,811,507 - emrB b2686 0.56 -3.3
2,811,507 - emrB b2686 0.56 -3.1
2,811,528 + +2.0
2,811,581 - emrB b2686 0.61 -2.8

Or see this region's nucleotide sequence