Strain Fitness in Escherichia coli BW25113 around b2541

Experiment: octanol 0.08 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthcaE and hcaF overlap by 4 nucleotideshcaF and hcaC overlap by 1 nucleotideshcaC and hcaB overlap by 4 nucleotideshcaB and hcaD are separated by 9 nucleotides b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415 hcaE b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930 hcaF b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250 hcaC b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059 hcaB b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271 hcaD Position (kb) 2669 2670 2671Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2668.253 kb on + strand, within hcaEat 2668.253 kb on + strand, within hcaEat 2668.363 kb on + strandat 2668.539 kb on - strand, within hcaFat 2668.551 kb on - strand, within hcaFat 2668.551 kb on - strand, within hcaFat 2668.676 kb on + strand, within hcaFat 2668.676 kb on + strand, within hcaFat 2668.709 kb on + strand, within hcaFat 2668.715 kb on + strand, within hcaFat 2668.736 kb on - strand, within hcaFat 2668.759 kb on + strand, within hcaFat 2668.888 kb on - strandat 2668.888 kb on - strandat 2668.925 kb on - strandat 2668.932 kb on - strandat 2668.979 kb on - strand, within hcaCat 2669.083 kb on + strand, within hcaCat 2669.112 kb on - strand, within hcaCat 2669.166 kb on + strand, within hcaCat 2669.295 kb on + strandat 2669.442 kb on - strand, within hcaBat 2669.469 kb on - strandat 2669.662 kb on + strand, within hcaBat 2669.678 kb on - strand, within hcaBat 2669.678 kb on - strand, within hcaBat 2669.716 kb on - strand, within hcaBat 2669.716 kb on - strand, within hcaBat 2669.827 kb on + strand, within hcaBat 2669.827 kb on + strand, within hcaBat 2669.835 kb on - strand, within hcaBat 2669.835 kb on - strand, within hcaBat 2669.946 kb on + strand, within hcaBat 2669.947 kb on - strand, within hcaBat 2669.951 kb on + strand, within hcaBat 2669.951 kb on + strand, within hcaBat 2670.153 kb on + strandat 2670.199 kb on + strand, within hcaDat 2670.427 kb on - strand, within hcaDat 2670.427 kb on - strand, within hcaDat 2670.447 kb on + strand, within hcaDat 2670.450 kb on - strand, within hcaDat 2670.450 kb on - strand, within hcaDat 2670.576 kb on - strand, within hcaDat 2670.576 kb on - strand, within hcaDat 2670.576 kb on - strand, within hcaDat 2670.603 kb on + strand, within hcaDat 2670.836 kb on - strand, within hcaDat 2670.927 kb on - strand, within hcaDat 2670.994 kb on - strand, within hcaDat 2670.994 kb on - strand, within hcaDat 2671.037 kb on + strand, within hcaD

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Per-strain Table

Position Strand Gene LocusTag Fraction octanol 0.08 vol%
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2,668,253 + hcaE b2538 0.88 -1.1
2,668,253 + hcaE b2538 0.88 -0.5
2,668,363 + +3.4
2,668,539 - hcaF b2539 0.24 -2.1
2,668,551 - hcaF b2539 0.27 +1.2
2,668,551 - hcaF b2539 0.27 +1.6
2,668,676 + hcaF b2539 0.51 +0.4
2,668,676 + hcaF b2539 0.51 +1.5
2,668,709 + hcaF b2539 0.57 -1.4
2,668,715 + hcaF b2539 0.58 +0.0
2,668,736 - hcaF b2539 0.62 -4.2
2,668,759 + hcaF b2539 0.67 +0.4
2,668,888 - +0.6
2,668,888 - +1.8
2,668,925 - +0.3
2,668,932 - -3.5
2,668,979 - hcaC b2540 0.15 +0.6
2,669,083 + hcaC b2540 0.48 -0.3
2,669,112 - hcaC b2540 0.57 -1.8
2,669,166 + hcaC b2540 0.74 +1.9
2,669,295 + +0.5
2,669,442 - hcaB b2541 0.24 -0.7
2,669,469 - +0.4
2,669,662 + hcaB b2541 0.51 -0.5
2,669,678 - hcaB b2541 0.53 -0.1
2,669,678 - hcaB b2541 0.53 -0.9
2,669,716 - hcaB b2541 0.58 +2.0
2,669,716 - hcaB b2541 0.58 -1.2
2,669,827 + hcaB b2541 0.71 +0.3
2,669,827 + hcaB b2541 0.71 -2.3
2,669,835 - hcaB b2541 0.72 +0.7
2,669,835 - hcaB b2541 0.72 -2.2
2,669,946 + hcaB b2541 0.86 -1.9
2,669,947 - hcaB b2541 0.86 +0.3
2,669,951 + hcaB b2541 0.87 +0.2
2,669,951 + hcaB b2541 0.87 +1.5
2,670,153 + -2.8
2,670,199 + hcaD b2542 0.11 -1.3
2,670,427 - hcaD b2542 0.30 +1.0
2,670,427 - hcaD b2542 0.30 -0.3
2,670,447 + hcaD b2542 0.31 +0.9
2,670,450 - hcaD b2542 0.32 +2.0
2,670,450 - hcaD b2542 0.32 -1.5
2,670,576 - hcaD b2542 0.42 +0.4
2,670,576 - hcaD b2542 0.42 +0.5
2,670,576 - hcaD b2542 0.42 +0.1
2,670,603 + hcaD b2542 0.44 +1.2
2,670,836 - hcaD b2542 0.64 +1.3
2,670,927 - hcaD b2542 0.71 +0.9
2,670,994 - hcaD b2542 0.77 +2.1
2,670,994 - hcaD b2542 0.77 +0.1
2,671,037 + hcaD b2542 0.80 +1.1

Or see this region's nucleotide sequence