Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dedA and truA are separated by 82 nucleotides truA and usg overlap by 1 nucleotides usg and pdxB are separated by 65 nucleotides
b2317: dedA - conserved inner membrane protein (NCBI), at 2,432,104 to 2,432,763
dedA
b2318: truA - tRNA pseudouridine synthase A (NCBI), at 2,432,846 to 2,433,658
truA
b2319: usg - hypothetical protein (NCBI), at 2,433,658 to 2,434,671
usg
b2320: pdxB - erythronate-4-phosphate dehydrogenase (NCBI), at 2,434,737 to 2,435,873
pdxB
Position (kb)
2433
2434
2435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2432.800 kb on + strand at 2432.808 kb on - strand at 2432.808 kb on - strand at 2433.004 kb on + strand, within truA at 2433.141 kb on + strand, within truA at 2433.141 kb on + strand, within truA at 2433.157 kb on + strand, within truA at 2433.160 kb on - strand, within truA at 2433.160 kb on - strand, within truA at 2433.170 kb on - strand, within truA at 2433.363 kb on - strand at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.600 kb on + strand at 2433.600 kb on + strand at 2433.656 kb on + strand at 2433.664 kb on - strand at 2433.670 kb on + strand at 2433.670 kb on + strand at 2433.678 kb on - strand at 2433.753 kb on - strand at 2433.846 kb on + strand, within usg at 2433.853 kb on - strand, within usg at 2433.853 kb on - strand, within usg at 2433.910 kb on + strand, within usg at 2433.943 kb on + strand, within usg at 2433.952 kb on - strand, within usg at 2433.952 kb on - strand, within usg at 2434.057 kb on + strand, within usg at 2434.167 kb on - strand, within usg at 2434.287 kb on - strand, within usg at 2434.287 kb on - strand, within usg at 2434.339 kb on + strand, within usg at 2434.360 kb on + strand, within usg at 2434.626 kb on - strand at 2434.647 kb on + strand at 2434.647 kb on + strand at 2434.690 kb on - strand at 2434.711 kb on + strand at 2434.761 kb on + strand at 2434.787 kb on + strand at 2434.881 kb on - strand, within pdxB at 2434.881 kb on - strand, within pdxB at 2434.932 kb on + strand, within pdxB at 2434.932 kb on + strand, within pdxB at 2434.999 kb on - strand, within pdxB at 2435.038 kb on - strand, within pdxB at 2435.105 kb on - strand at 2435.110 kb on + strand, within pdxB at 2435.110 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.230 kb on + strand, within pdxB at 2435.246 kb on + strand, within pdxB at 2435.285 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.324 kb on + strand, within pdxB at 2435.343 kb on - strand, within pdxB at 2435.511 kb on + strand, within pdxB
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 2,432,800 + -3.3 2,432,808 - +2.9 2,432,808 - -1.0 2,433,004 + truA b2318 0.19 +3.8 2,433,141 + truA b2318 0.36 +1.9 2,433,141 + truA b2318 0.36 +1.6 2,433,157 + truA b2318 0.38 +3.2 2,433,160 - truA b2318 0.39 +5.2 2,433,160 - truA b2318 0.39 +3.5 2,433,170 - truA b2318 0.40 -1.2 2,433,363 - +4.7 2,433,465 - truA b2318 0.76 +3.0 2,433,465 - truA b2318 0.76 +3.5 2,433,465 - truA b2318 0.76 +2.5 2,433,465 - truA b2318 0.76 +4.3 2,433,600 + +3.7 2,433,600 + +2.0 2,433,656 + +2.0 2,433,664 - +2.1 2,433,670 + +1.9 2,433,670 + +1.2 2,433,678 - +4.1 2,433,753 - +3.3 2,433,846 + usg b2319 0.19 +2.3 2,433,853 - usg b2319 0.19 +2.3 2,433,853 - usg b2319 0.19 +1.6 2,433,910 + usg b2319 0.25 -2.5 2,433,943 + usg b2319 0.28 +0.5 2,433,952 - usg b2319 0.29 +0.1 2,433,952 - usg b2319 0.29 +3.2 2,434,057 + usg b2319 0.39 +3.4 2,434,167 - usg b2319 0.50 -0.3 2,434,287 - usg b2319 0.62 +0.1 2,434,287 - usg b2319 0.62 +3.6 2,434,339 + usg b2319 0.67 +2.7 2,434,360 + usg b2319 0.69 +4.0 2,434,626 - +2.3 2,434,647 + +2.7 2,434,647 + +3.6 2,434,690 - -0.1 2,434,711 + +2.6 2,434,761 + +2.3 2,434,787 + -1.6 2,434,881 - pdxB b2320 0.13 +1.4 2,434,881 - pdxB b2320 0.13 +3.0 2,434,932 + pdxB b2320 0.17 +0.8 2,434,932 + pdxB b2320 0.17 -1.1 2,434,999 - pdxB b2320 0.23 +0.2 2,435,038 - pdxB b2320 0.26 -0.3 2,435,105 - +3.7 2,435,110 + pdxB b2320 0.33 +2.2 2,435,110 + pdxB b2320 0.33 -3.4 2,435,230 + pdxB b2320 0.43 +0.7 2,435,230 + pdxB b2320 0.43 -2.2 2,435,246 + pdxB b2320 0.45 -2.2 2,435,285 + pdxB b2320 0.48 +0.0 2,435,324 + pdxB b2320 0.52 -0.9 2,435,324 + pdxB b2320 0.52 +0.1 2,435,343 - pdxB b2320 0.53 +2.0 2,435,511 + pdxB b2320 0.68 +1.7
Or see this region's nucleotide sequence