Strain Fitness in Escherichia coli BW25113 around b2137

Experiment: octanol 0.08 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyohC and yohD are separated by 169 nucleotidesyohD and yohF are separated by 129 nucleotidesyohF and mdtQ are separated by 52 nucleotides b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653 yohC b2136: yohD - orf, hypothetical protein (VIMSS), at 2,223,823 to 2,224,401 yohD b2137: yohF - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,224,531 to 2,225,292 yohF b2139: mdtQ - orf, hypothetical protein (VIMSS), at 2,225,345 to 2,226,780 mdtQ Position (kb) 2224 2225 2226Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2223.531 kb on + strand, within yohCat 2223.573 kb on - strand, within yohCat 2223.647 kb on + strandat 2223.831 kb on + strandat 2223.879 kb on + strandat 2224.007 kb on - strand, within yohDat 2224.171 kb on - strand, within yohDat 2224.249 kb on - strand, within yohDat 2224.348 kb on - strandat 2224.358 kb on + strandat 2224.417 kb on - strandat 2224.529 kb on - strandat 2224.541 kb on - strandat 2224.578 kb on + strandat 2224.713 kb on - strand, within yohFat 2224.872 kb on - strand, within yohFat 2224.902 kb on + strand, within yohFat 2224.902 kb on + strand, within yohFat 2224.942 kb on + strand, within yohFat 2224.992 kb on - strand, within yohFat 2224.992 kb on - strand, within yohFat 2224.994 kb on - strand, within yohFat 2224.994 kb on - strand, within yohFat 2225.063 kb on - strand, within yohFat 2225.103 kb on - strand, within yohFat 2225.133 kb on + strand, within yohFat 2225.163 kb on + strand, within yohFat 2225.199 kb on - strand, within yohFat 2225.292 kb on - strandat 2225.409 kb on + strandat 2225.429 kb on - strandat 2225.476 kb on - strandat 2225.538 kb on + strand, within mdtQat 2225.672 kb on - strand, within mdtQat 2225.711 kb on + strand, within mdtQat 2225.719 kb on - strand, within mdtQat 2225.756 kb on - strand, within mdtQat 2225.756 kb on - strand, within mdtQat 2225.777 kb on + strand, within mdtQat 2225.795 kb on + strand, within mdtQat 2225.795 kb on + strand, within mdtQat 2225.843 kb on + strand, within mdtQat 2225.862 kb on + strand, within mdtQat 2225.862 kb on + strand, within mdtQat 2225.867 kb on - strand, within mdtQat 2225.899 kb on + strand, within mdtQat 2225.899 kb on + strand, within mdtQat 2225.906 kb on + strand, within mdtQat 2225.917 kb on - strand, within mdtQat 2225.964 kb on - strand, within mdtQat 2225.964 kb on - strand, within mdtQat 2225.970 kb on + strand, within mdtQat 2226.046 kb on - strand, within mdtQat 2226.046 kb on - strand, within mdtQat 2226.097 kb on + strand, within mdtQat 2226.097 kb on + strand, within mdtQat 2226.118 kb on + strand, within mdtQat 2226.126 kb on - strand, within mdtQ

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Per-strain Table

Position Strand Gene LocusTag Fraction octanol 0.08 vol%
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2,223,531 + yohC b2135 0.79 +0.5
2,223,573 - yohC b2135 0.86 -0.2
2,223,647 + -0.3
2,223,831 + -1.4
2,223,879 + +0.2
2,224,007 - yohD b2136 0.32 -0.1
2,224,171 - yohD b2136 0.60 -0.1
2,224,249 - yohD b2136 0.74 +1.0
2,224,348 - +0.9
2,224,358 + -1.5
2,224,417 - -1.1
2,224,529 - -0.9
2,224,541 - -1.0
2,224,578 + -1.3
2,224,713 - yohF b2137 0.24 +2.0
2,224,872 - yohF b2137 0.45 +0.7
2,224,902 + yohF b2137 0.49 -0.9
2,224,902 + yohF b2137 0.49 +2.9
2,224,942 + yohF b2137 0.54 -2.9
2,224,992 - yohF b2137 0.60 -1.2
2,224,992 - yohF b2137 0.60 +0.3
2,224,994 - yohF b2137 0.61 +3.1
2,224,994 - yohF b2137 0.61 +0.6
2,225,063 - yohF b2137 0.70 -3.3
2,225,103 - yohF b2137 0.75 +2.0
2,225,133 + yohF b2137 0.79 +2.2
2,225,163 + yohF b2137 0.83 +0.7
2,225,199 - yohF b2137 0.88 -2.4
2,225,292 - -2.9
2,225,409 + +1.2
2,225,429 - +0.0
2,225,476 - +0.7
2,225,538 + mdtQ b2139 0.13 +0.2
2,225,672 - mdtQ b2139 0.23 -2.7
2,225,711 + mdtQ b2139 0.25 -0.6
2,225,719 - mdtQ b2139 0.26 -0.2
2,225,756 - mdtQ b2139 0.29 -1.7
2,225,756 - mdtQ b2139 0.29 -3.5
2,225,777 + mdtQ b2139 0.30 -0.4
2,225,795 + mdtQ b2139 0.31 -3.6
2,225,795 + mdtQ b2139 0.31 -0.7
2,225,843 + mdtQ b2139 0.35 -0.3
2,225,862 + mdtQ b2139 0.36 -1.9
2,225,862 + mdtQ b2139 0.36 -0.2
2,225,867 - mdtQ b2139 0.36 +0.3
2,225,899 + mdtQ b2139 0.39 -1.6
2,225,899 + mdtQ b2139 0.39 +1.1
2,225,906 + mdtQ b2139 0.39 +1.4
2,225,917 - mdtQ b2139 0.40 -0.1
2,225,964 - mdtQ b2139 0.43 -2.3
2,225,964 - mdtQ b2139 0.43 +1.5
2,225,970 + mdtQ b2139 0.44 +0.8
2,226,046 - mdtQ b2139 0.49 -0.3
2,226,046 - mdtQ b2139 0.49 -0.8
2,226,097 + mdtQ b2139 0.52 -0.2
2,226,097 + mdtQ b2139 0.52 -1.7
2,226,118 + mdtQ b2139 0.54 +0.3
2,226,126 - mdtQ b2139 0.54 +2.2

Or see this region's nucleotide sequence