Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliG and fliH overlap by 8 nucleotides fliH and fliI overlap by 1 nucleotides fliI and fliJ are separated by 18 nucleotides fliJ and fliK overlap by 4 nucleotides
b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899
fliG
b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578
fliH
b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951
fliI
b1942: fliJ - flagellar biosynthesis chaperone (NCBI), at 2,015,970 to 2,016,413
fliJ
b1943: fliK - flagellar hook-length control protein (NCBI), at 2,016,410 to 2,017,537
fliK
Position (kb)
2014
2015
2016 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2014.036 kb on + strand, within fliH at 2014.099 kb on - strand, within fliH at 2014.120 kb on + strand, within fliH at 2014.214 kb on + strand, within fliH at 2014.240 kb on - strand, within fliH at 2014.246 kb on + strand, within fliH at 2014.318 kb on - strand, within fliH at 2014.336 kb on + strand, within fliH at 2014.404 kb on - strand, within fliH at 2014.562 kb on + strand at 2014.562 kb on + strand at 2014.829 kb on + strand, within fliI at 2014.829 kb on + strand, within fliI at 2014.917 kb on - strand, within fliI at 2014.920 kb on + strand, within fliI at 2014.920 kb on + strand, within fliI at 2015.057 kb on - strand, within fliI at 2015.057 kb on - strand at 2015.259 kb on - strand, within fliI at 2015.261 kb on - strand, within fliI at 2015.261 kb on - strand, within fliI at 2015.499 kb on - strand, within fliI at 2015.549 kb on + strand, within fliI at 2015.549 kb on + strand, within fliI at 2015.560 kb on + strand, within fliI at 2015.631 kb on + strand, within fliI at 2015.685 kb on - strand, within fliI at 2015.685 kb on - strand, within fliI at 2015.701 kb on + strand at 2015.701 kb on + strand, within fliI at 2015.847 kb on - strand at 2015.847 kb on - strand at 2016.216 kb on - strand, within fliJ at 2016.216 kb on - strand, within fliJ at 2016.658 kb on - strand, within fliK at 2016.927 kb on - strand, within fliK
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 2,014,036 + fliH b1940 0.21 +0.2 2,014,099 - fliH b1940 0.30 -2.4 2,014,120 + fliH b1940 0.33 +3.0 2,014,214 + fliH b1940 0.47 +0.2 2,014,240 - fliH b1940 0.51 -0.4 2,014,246 + fliH b1940 0.52 -1.4 2,014,318 - fliH b1940 0.62 -1.4 2,014,336 + fliH b1940 0.65 +1.2 2,014,404 - fliH b1940 0.75 +0.8 2,014,562 + -0.5 2,014,562 + +4.1 2,014,829 + fliI b1941 0.18 +2.6 2,014,829 + fliI b1941 0.18 -0.9 2,014,917 - fliI b1941 0.25 +1.0 2,014,920 + fliI b1941 0.25 -0.5 2,014,920 + fliI b1941 0.25 +2.1 2,015,057 - fliI b1941 0.35 +0.4 2,015,057 - +0.4 2,015,259 - fliI b1941 0.50 +0.1 2,015,261 - fliI b1941 0.50 +3.5 2,015,261 - fliI b1941 0.50 +0.0 2,015,499 - fliI b1941 0.67 +1.2 2,015,549 + fliI b1941 0.71 +2.6 2,015,549 + fliI b1941 0.71 +2.0 2,015,560 + fliI b1941 0.71 +0.8 2,015,631 + fliI b1941 0.77 +1.6 2,015,685 - fliI b1941 0.81 +1.9 2,015,685 - fliI b1941 0.81 +2.2 2,015,701 + +1.4 2,015,701 + fliI b1941 0.82 -1.4 2,015,847 - +0.9 2,015,847 - -1.4 2,016,216 - fliJ b1942 0.55 +3.1 2,016,216 - fliJ b1942 0.55 +2.3 2,016,658 - fliK b1943 0.22 +3.5 2,016,927 - fliK b1943 0.46 +0.8
Or see this region's nucleotide sequence