Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt puuE and pspF are separated by 119 nucleotides pspF and pspA are separated by 166 nucleotides pspA and pspB are separated by 53 nucleotides
b1302: puuE - GABA aminotransferase, PLP-dependent (NCBI), at 1,363,574 to 1,364,839
puuE
b1303: pspF - psp operon transcriptional activator (VIMSS), at 1,364,959 to 1,365,936
pspF
b1304: pspA - regulatory protein for phage-shock-protein operon (NCBI), at 1,366,103 to 1,366,771
pspA
b1305: pspB - phage shock protein B (NCBI), at 1,366,825 to 1,367,049
pspB
Position (kb)
1364
1365
1366 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1363.993 kb on + strand, within puuE at 1364.468 kb on + strand, within puuE at 1364.619 kb on + strand, within puuE at 1364.739 kb on - strand at 1364.887 kb on - strand at 1364.951 kb on - strand at 1364.994 kb on + strand at 1365.094 kb on - strand, within pspF at 1365.094 kb on - strand, within pspF at 1365.177 kb on + strand, within pspF at 1365.234 kb on - strand, within pspF at 1365.275 kb on + strand, within pspF at 1365.299 kb on - strand, within pspF at 1365.299 kb on - strand, within pspF at 1365.402 kb on + strand, within pspF at 1365.559 kb on - strand, within pspF at 1365.559 kb on - strand, within pspF at 1365.591 kb on + strand, within pspF at 1365.850 kb on + strand at 1366.212 kb on + strand, within pspA at 1366.230 kb on - strand, within pspA at 1366.244 kb on + strand, within pspA at 1366.373 kb on - strand, within pspA at 1366.560 kb on + strand, within pspA at 1366.628 kb on + strand, within pspA at 1366.818 kb on - strand at 1366.840 kb on - strand at 1366.896 kb on + strand, within pspB at 1366.931 kb on + strand, within pspB
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 1,363,993 + puuE b1302 0.33 +1.4 1,364,468 + puuE b1302 0.71 -1.4 1,364,619 + puuE b1302 0.83 +0.0 1,364,739 - -2.5 1,364,887 - +0.7 1,364,951 - -0.2 1,364,994 + +0.9 1,365,094 - pspF b1303 0.14 +2.1 1,365,094 - pspF b1303 0.14 +1.5 1,365,177 + pspF b1303 0.22 -1.4 1,365,234 - pspF b1303 0.28 -0.8 1,365,275 + pspF b1303 0.32 +1.7 1,365,299 - pspF b1303 0.35 +1.7 1,365,299 - pspF b1303 0.35 -2.9 1,365,402 + pspF b1303 0.45 +0.6 1,365,559 - pspF b1303 0.61 -1.6 1,365,559 - pspF b1303 0.61 +1.3 1,365,591 + pspF b1303 0.65 +2.2 1,365,850 + -3.3 1,366,212 + pspA b1304 0.16 -2.6 1,366,230 - pspA b1304 0.19 -1.1 1,366,244 + pspA b1304 0.21 -2.4 1,366,373 - pspA b1304 0.40 +0.2 1,366,560 + pspA b1304 0.68 -1.5 1,366,628 + pspA b1304 0.78 -1.0 1,366,818 - +0.5 1,366,840 - -0.1 1,366,896 + pspB b1305 0.32 +2.7 1,366,931 + pspB b1305 0.47 +1.9
Or see this region's nucleotide sequence