Strain Fitness in Escherichia coli BW25113 around b1296

Experiment: octanol 0.08 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsapA and ymjA are separated by 312 nucleotidesymjA and ycjJ are separated by 133 nucleotidesycjJ and b1297 are separated by 302 nucleotides b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134 sapA b1295: ymjA - hypothetical protein (NCBI), at 1,355,447 to 1,355,692 ymjA b1296: ycjJ - putative amino acid/amine transport protein (VIMSS), at 1,355,826 to 1,357,211 ycjJ b1297: b1297 - putative glutamine synthetase (EC 6.3.1.2) (VIMSS), at 1,357,514 to 1,358,932 b1297 Position (kb) 1355 1356 1357 1358Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1354.915 kb on - strand, within sapAat 1354.915 kb on - strand, within sapAat 1354.998 kb on - strandat 1355.215 kb on + strandat 1355.225 kb on - strandat 1355.225 kb on - strandat 1355.370 kb on - strandat 1355.370 kb on - strandat 1355.445 kb on - strandat 1355.485 kb on + strand, within ymjAat 1355.488 kb on - strand, within ymjAat 1355.488 kb on - strand, within ymjAat 1355.507 kb on - strand, within ymjAat 1355.713 kb on - strandat 1355.723 kb on - strandat 1355.726 kb on - strandat 1355.728 kb on - strandat 1355.853 kb on - strandat 1355.862 kb on + strandat 1356.256 kb on + strand, within ycjJat 1356.736 kb on - strand, within ycjJat 1356.934 kb on - strand, within ycjJat 1356.934 kb on - strand, within ycjJat 1356.948 kb on + strand, within ycjJat 1356.948 kb on + strand, within ycjJat 1356.949 kb on + strand, within ycjJat 1356.949 kb on + strand, within ycjJat 1357.116 kb on - strandat 1357.116 kb on - strandat 1357.150 kb on + strandat 1357.189 kb on - strandat 1357.489 kb on - strandat 1357.696 kb on - strand, within b1297at 1357.696 kb on - strand, within b1297at 1357.820 kb on + strand, within b1297at 1357.845 kb on - strand, within b1297at 1357.845 kb on - strand, within b1297at 1357.898 kb on - strand, within b1297

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Per-strain Table

Position Strand Gene LocusTag Fraction octanol 0.08 vol%
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1,354,915 - sapA b1294 0.87 -2.2
1,354,915 - sapA b1294 0.87 +1.3
1,354,998 - -0.1
1,355,215 + +1.1
1,355,225 - -0.3
1,355,225 - +1.6
1,355,370 - +0.4
1,355,370 - -1.0
1,355,445 - +0.0
1,355,485 + ymjA b1295 0.15 -0.5
1,355,488 - ymjA b1295 0.17 -1.2
1,355,488 - ymjA b1295 0.17 -0.5
1,355,507 - ymjA b1295 0.24 -1.2
1,355,713 - -0.8
1,355,723 - +1.6
1,355,726 - -1.7
1,355,728 - +3.0
1,355,853 - -0.9
1,355,862 + -3.0
1,356,256 + ycjJ b1296 0.31 -1.2
1,356,736 - ycjJ b1296 0.66 -1.2
1,356,934 - ycjJ b1296 0.80 +2.8
1,356,934 - ycjJ b1296 0.80 -1.9
1,356,948 + ycjJ b1296 0.81 +3.9
1,356,948 + ycjJ b1296 0.81 +2.0
1,356,949 + ycjJ b1296 0.81 -2.1
1,356,949 + ycjJ b1296 0.81 -2.7
1,357,116 - -0.3
1,357,116 - -1.0
1,357,150 + +1.4
1,357,189 - +2.3
1,357,489 - +0.1
1,357,696 - b1297 b1297 0.13 -2.8
1,357,696 - b1297 b1297 0.13 +1.1
1,357,820 + b1297 b1297 0.22 -1.0
1,357,845 - b1297 b1297 0.23 -0.4
1,357,845 - b1297 b1297 0.23 +1.1
1,357,898 - b1297 b1297 0.27 -0.0

Or see this region's nucleotide sequence