Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potC and potB overlap by 4 nucleotides potB and potA are separated by 13 nucleotides potA and pepT are separated by 249 nucleotides
b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843
potC
b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667
potB
b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817
potA
b1127: pepT - peptidase T (NCBI), at 1,185,067 to 1,186,293
pepT
Position (kb)
1183
1184
1185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1182.691 kb on + strand, within potC at 1182.700 kb on + strand, within potC at 1182.730 kb on + strand, within potC at 1182.910 kb on + strand at 1182.910 kb on + strand at 1182.914 kb on - strand at 1183.032 kb on + strand, within potB at 1183.032 kb on + strand, within potB at 1183.193 kb on - strand, within potB at 1183.202 kb on + strand, within potB at 1183.202 kb on + strand, within potB at 1183.283 kb on + strand, within potB at 1183.449 kb on + strand, within potB at 1183.542 kb on - strand, within potB at 1183.680 kb on - strand at 1183.698 kb on - strand at 1183.698 kb on - strand at 1183.711 kb on - strand at 1183.711 kb on - strand at 1183.884 kb on + strand, within potA at 1183.934 kb on - strand, within potA at 1184.015 kb on + strand, within potA at 1184.139 kb on - strand, within potA at 1184.163 kb on + strand, within potA at 1184.163 kb on + strand, within potA at 1184.286 kb on + strand, within potA at 1184.293 kb on + strand, within potA at 1184.334 kb on - strand, within potA at 1184.361 kb on - strand, within potA at 1184.599 kb on - strand, within potA at 1184.666 kb on + strand, within potA at 1184.692 kb on + strand, within potA at 1184.712 kb on + strand at 1184.724 kb on + strand at 1184.732 kb on - strand at 1184.847 kb on + strand at 1185.128 kb on + strand at 1185.135 kb on - strand at 1185.135 kb on - strand at 1185.153 kb on + strand at 1185.190 kb on + strand, within pepT at 1185.276 kb on + strand, within pepT at 1185.296 kb on + strand, within pepT at 1185.409 kb on + strand, within pepT at 1185.639 kb on + strand, within pepT at 1185.647 kb on + strand, within pepT at 1185.720 kb on + strand, within pepT at 1185.746 kb on - strand, within pepT at 1185.779 kb on - strand, within pepT
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 1,182,691 + potC b1124 0.81 +0.8 1,182,700 + potC b1124 0.82 -3.1 1,182,730 + potC b1124 0.86 -1.2 1,182,910 + -3.7 1,182,910 + -1.4 1,182,914 - +2.4 1,183,032 + potB b1125 0.23 +2.0 1,183,032 + potB b1125 0.23 +0.1 1,183,193 - potB b1125 0.43 -0.6 1,183,202 + potB b1125 0.44 -0.8 1,183,202 + potB b1125 0.44 -2.4 1,183,283 + potB b1125 0.54 -2.2 1,183,449 + potB b1125 0.74 +0.2 1,183,542 - potB b1125 0.85 -0.6 1,183,680 - -1.7 1,183,698 - -2.9 1,183,698 - +0.2 1,183,711 - -1.3 1,183,711 - -1.2 1,183,884 + potA b1126 0.18 -1.3 1,183,934 - potA b1126 0.22 -1.8 1,184,015 + potA b1126 0.29 -2.2 1,184,139 - potA b1126 0.40 -1.9 1,184,163 + potA b1126 0.42 -1.9 1,184,163 + potA b1126 0.42 -1.3 1,184,286 + potA b1126 0.53 -2.2 1,184,293 + potA b1126 0.54 -2.0 1,184,334 - potA b1126 0.57 -2.5 1,184,361 - potA b1126 0.60 -3.3 1,184,599 - potA b1126 0.81 -2.2 1,184,666 + potA b1126 0.87 -2.3 1,184,692 + potA b1126 0.89 -0.4 1,184,712 + -2.4 1,184,724 + -0.1 1,184,732 - -0.1 1,184,847 + +2.5 1,185,128 + -0.8 1,185,135 - +1.5 1,185,135 - +2.1 1,185,153 + +0.5 1,185,190 + pepT b1127 0.10 +1.2 1,185,276 + pepT b1127 0.17 +0.3 1,185,296 + pepT b1127 0.19 +0.1 1,185,409 + pepT b1127 0.28 -1.3 1,185,639 + pepT b1127 0.47 +0.3 1,185,647 + pepT b1127 0.47 +0.5 1,185,720 + pepT b1127 0.53 +1.0 1,185,746 - pepT b1127 0.55 -1.0 1,185,779 - pepT b1127 0.58 -2.7
Or see this region's nucleotide sequence