Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycfZ and ymfA overlap by 4 nucleotides ymfA and potD are separated by 57 nucleotides potD and potC overlap by 4 nucleotides potC and potB overlap by 4 nucleotides
b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490
ycfZ
b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948
ymfA
b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052
potD
b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843
potC
b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667
potB
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1180.234 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.438 kb on + strand at 1180.457 kb on + strand at 1180.739 kb on - strand, within ymfA at 1180.755 kb on - strand, within ymfA at 1180.755 kb on - strand, within ymfA at 1180.763 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.778 kb on - strand, within ymfA at 1180.796 kb on + strand, within ymfA at 1180.796 kb on + strand, within ymfA at 1180.799 kb on - strand, within ymfA at 1180.803 kb on - strand, within ymfA at 1180.809 kb on + strand, within ymfA at 1180.813 kb on + strand, within ymfA at 1180.817 kb on - strand, within ymfA at 1180.834 kb on - strand, within ymfA at 1180.838 kb on - strand, within ymfA at 1180.854 kb on - strand, within ymfA at 1180.869 kb on - strand, within ymfA at 1180.881 kb on + strand, within ymfA at 1180.881 kb on + strand, within ymfA at 1180.890 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.900 kb on + strand, within ymfA at 1180.900 kb on + strand, within ymfA at 1180.902 kb on - strand at 1180.908 kb on - strand at 1180.937 kb on + strand at 1180.945 kb on - strand at 1180.945 kb on - strand at 1180.953 kb on - strand at 1180.959 kb on - strand at 1180.988 kb on - strand at 1180.988 kb on - strand at 1180.989 kb on - strand at 1180.989 kb on - strand at 1181.036 kb on - strand at 1181.046 kb on - strand at 1181.101 kb on + strand at 1181.101 kb on + strand at 1181.170 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.421 kb on + strand, within potD at 1181.480 kb on - strand, within potD at 1181.522 kb on - strand, within potD at 1181.539 kb on + strand, within potD at 1181.547 kb on - strand, within potD at 1181.547 kb on - strand, within potD at 1181.556 kb on + strand, within potD at 1181.556 kb on + strand, within potD at 1181.581 kb on - strand, within potD at 1181.684 kb on - strand, within potD at 1181.721 kb on - strand, within potD at 1181.839 kb on - strand, within potD at 1181.865 kb on - strand, within potD at 1181.918 kb on + strand, within potD at 1182.003 kb on + strand at 1182.395 kb on + strand, within potC at 1182.474 kb on - strand, within potC at 1182.476 kb on - strand, within potC at 1182.524 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.589 kb on + strand, within potC at 1182.668 kb on + strand, within potC at 1182.691 kb on + strand, within potC at 1182.700 kb on + strand, within potC at 1182.730 kb on + strand, within potC at 1182.910 kb on + strand at 1182.910 kb on + strand at 1182.914 kb on - strand at 1183.032 kb on + strand, within potB at 1183.032 kb on + strand, within potB
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 1,180,234 - ycfZ b1121 0.67 -2.0 1,180,389 - ycfZ b1121 0.87 +1.4 1,180,389 - ycfZ b1121 0.87 -0.9 1,180,438 + -0.3 1,180,457 + +2.1 1,180,739 - ymfA b1122 0.55 +1.8 1,180,755 - ymfA b1122 0.58 +1.4 1,180,755 - ymfA b1122 0.58 -3.4 1,180,763 - ymfA b1122 0.60 -1.8 1,180,777 - ymfA b1122 0.63 +0.5 1,180,777 - ymfA b1122 0.63 -2.5 1,180,777 - ymfA b1122 0.63 +1.0 1,180,777 - ymfA b1122 0.63 +1.1 1,180,777 - ymfA b1122 0.63 +1.6 1,180,777 - ymfA b1122 0.63 -0.2 1,180,778 - ymfA b1122 0.63 +2.8 1,180,796 + ymfA b1122 0.67 -0.3 1,180,796 + ymfA b1122 0.67 +0.4 1,180,799 - ymfA b1122 0.68 +0.9 1,180,803 - ymfA b1122 0.68 +1.1 1,180,809 + ymfA b1122 0.70 -0.4 1,180,813 + ymfA b1122 0.71 -2.9 1,180,817 - ymfA b1122 0.71 +0.7 1,180,834 - ymfA b1122 0.75 -2.1 1,180,838 - ymfA b1122 0.76 +0.4 1,180,854 - ymfA b1122 0.79 -1.9 1,180,869 - ymfA b1122 0.83 +2.2 1,180,881 + ymfA b1122 0.85 +1.5 1,180,881 + ymfA b1122 0.85 +2.7 1,180,890 + ymfA b1122 0.87 -0.3 1,180,894 + ymfA b1122 0.88 +0.7 1,180,894 + ymfA b1122 0.88 +1.4 1,180,894 + ymfA b1122 0.88 -3.4 1,180,900 + ymfA b1122 0.89 +1.0 1,180,900 + ymfA b1122 0.89 -3.7 1,180,902 - -0.5 1,180,908 - +1.7 1,180,937 + +0.6 1,180,945 - +0.3 1,180,945 - +0.4 1,180,953 - +0.5 1,180,959 - +1.1 1,180,988 - +0.9 1,180,988 - +3.4 1,180,989 - +1.9 1,180,989 - +1.2 1,181,036 - -0.6 1,181,046 - +2.1 1,181,101 + -2.4 1,181,101 + -0.5 1,181,170 - potD b1123 0.16 -1.7 1,181,280 - potD b1123 0.26 -0.4 1,181,280 - potD b1123 0.26 -1.2 1,181,421 + potD b1123 0.40 -1.2 1,181,480 - potD b1123 0.45 -1.2 1,181,522 - potD b1123 0.49 -1.9 1,181,539 + potD b1123 0.51 +0.5 1,181,547 - potD b1123 0.52 -2.4 1,181,547 - potD b1123 0.52 -0.7 1,181,556 + potD b1123 0.53 -0.6 1,181,556 + potD b1123 0.53 -1.4 1,181,581 - potD b1123 0.55 -2.5 1,181,684 - potD b1123 0.65 -2.6 1,181,721 - potD b1123 0.68 -1.7 1,181,839 - potD b1123 0.80 -2.1 1,181,865 - potD b1123 0.82 -0.6 1,181,918 + potD b1123 0.87 -2.7 1,182,003 + -1.0 1,182,395 + potC b1124 0.44 -2.6 1,182,474 - potC b1124 0.53 -1.5 1,182,476 - potC b1124 0.54 -1.0 1,182,524 + potC b1124 0.60 +0.1 1,182,581 + potC b1124 0.67 -0.5 1,182,581 + potC b1124 0.67 -3.6 1,182,589 + potC b1124 0.68 -1.9 1,182,668 + potC b1124 0.78 -2.7 1,182,691 + potC b1124 0.81 +0.8 1,182,700 + potC b1124 0.82 -3.1 1,182,730 + potC b1124 0.86 -1.2 1,182,910 + -3.7 1,182,910 + -1.4 1,182,914 - +2.4 1,183,032 + potB b1125 0.23 +2.0 1,183,032 + potB b1125 0.23 +0.1
Or see this region's nucleotide sequence