Strain Fitness in Escherichia coli BW25113 around b0153

Experiment: octanol 0.08 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfhuC and fhuD overlap by 1 nucleotidesfhuD and fhuB overlap by 4 nucleotidesfhuB and hemL are separated by 157 nucleotides b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575 fhuC b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465 fhuD b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444 fhuB b0154: hemL - glutamate-1-semialdehyde aminotransferase (NCBI), at 173,602 to 174,882 hemL Position (kb) 171 172 173 174Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 170.654 kb on - strandat 170.671 kb on + strand, within fhuDat 170.671 kb on + strand, within fhuDat 170.689 kb on - strand, within fhuDat 170.739 kb on + strand, within fhuDat 170.739 kb on + strand, within fhuDat 170.755 kb on - strand, within fhuDat 170.755 kb on - strand, within fhuDat 170.775 kb on + strand, within fhuDat 170.831 kb on + strand, within fhuDat 170.869 kb on + strand, within fhuDat 170.869 kb on + strand, within fhuDat 170.895 kb on - strand, within fhuDat 170.895 kb on - strand, within fhuDat 170.972 kb on - strand, within fhuDat 171.065 kb on + strand, within fhuDat 171.199 kb on + strand, within fhuDat 171.199 kb on + strand, within fhuDat 171.219 kb on + strand, within fhuDat 171.225 kb on + strand, within fhuDat 171.225 kb on + strand, within fhuDat 171.228 kb on + strand, within fhuDat 171.228 kb on + strand, within fhuDat 171.238 kb on - strand, within fhuDat 171.238 kb on - strand, within fhuDat 171.307 kb on - strand, within fhuDat 171.336 kb on + strand, within fhuDat 171.336 kb on + strand, within fhuDat 171.423 kb on + strandat 171.423 kb on - strandat 171.423 kb on - strandat 171.506 kb on + strandat 171.514 kb on - strandat 171.533 kb on - strandat 171.678 kb on + strand, within fhuBat 171.678 kb on + strand, within fhuBat 171.680 kb on + strand, within fhuBat 171.680 kb on + strand, within fhuBat 171.680 kb on - strand, within fhuBat 171.680 kb on - strand, within fhuBat 171.695 kb on + strand, within fhuBat 171.742 kb on + strand, within fhuBat 171.794 kb on + strand, within fhuBat 171.867 kb on + strand, within fhuBat 171.910 kb on + strand, within fhuBat 171.910 kb on + strand, within fhuBat 172.063 kb on + strand, within fhuBat 172.088 kb on - strand, within fhuBat 172.202 kb on + strand, within fhuBat 172.218 kb on - strand, within fhuBat 172.222 kb on - strand, within fhuBat 172.261 kb on + strand, within fhuBat 172.281 kb on + strand, within fhuBat 172.281 kb on + strand, within fhuBat 172.289 kb on + strand, within fhuBat 172.290 kb on - strand, within fhuBat 172.293 kb on + strand, within fhuBat 172.293 kb on + strand, within fhuBat 172.314 kb on + strand, within fhuBat 172.369 kb on - strand, within fhuBat 172.543 kb on - strand, within fhuBat 172.543 kb on - strand, within fhuBat 172.548 kb on + strand, within fhuBat 172.569 kb on - strand, within fhuBat 172.581 kb on + strand, within fhuBat 172.687 kb on + strand, within fhuBat 172.695 kb on - strand, within fhuBat 172.723 kb on + strand, within fhuBat 172.731 kb on - strand, within fhuBat 172.734 kb on + strand, within fhuBat 172.755 kb on + strand, within fhuBat 172.767 kb on + strand, within fhuBat 172.767 kb on + strand, within fhuBat 172.823 kb on - strand, within fhuBat 172.823 kb on - strand, within fhuBat 172.922 kb on - strand, within fhuBat 172.930 kb on + strand, within fhuBat 172.946 kb on - strand, within fhuBat 172.946 kb on - strand, within fhuBat 173.030 kb on + strand, within fhuBat 173.122 kb on - strand, within fhuBat 173.122 kb on - strand, within fhuBat 173.126 kb on - strand, within fhuBat 173.209 kb on + strand, within fhuBat 173.232 kb on - strand, within fhuBat 173.241 kb on + strand, within fhuBat 173.280 kb on + strandat 173.280 kb on + strandat 173.288 kb on - strandat 173.288 kb on - strandat 173.329 kb on + strandat 173.378 kb on - strandat 173.392 kb on + strandat 173.401 kb on - strandat 173.401 kb on - strandat 173.463 kb on + strandat 173.463 kb on - strandat 173.468 kb on + strandat 173.478 kb on + strandat 173.600 kb on + strandat 173.600 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction octanol 0.08 vol%
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170,654 - +1.7
170,671 + fhuD b0152 0.11 +1.4
170,671 + fhuD b0152 0.11 -3.3
170,689 - fhuD b0152 0.13 -0.2
170,739 + fhuD b0152 0.18 +0.3
170,739 + fhuD b0152 0.18 +0.7
170,755 - fhuD b0152 0.20 -1.3
170,755 - fhuD b0152 0.20 -1.6
170,775 + fhuD b0152 0.22 +1.5
170,831 + fhuD b0152 0.29 +0.4
170,869 + fhuD b0152 0.33 -0.3
170,869 + fhuD b0152 0.33 -3.0
170,895 - fhuD b0152 0.36 -1.8
170,895 - fhuD b0152 0.36 +2.9
170,972 - fhuD b0152 0.45 -0.7
171,065 + fhuD b0152 0.55 +1.1
171,199 + fhuD b0152 0.70 -0.7
171,199 + fhuD b0152 0.70 -2.7
171,219 + fhuD b0152 0.72 +0.4
171,225 + fhuD b0152 0.73 +0.1
171,225 + fhuD b0152 0.73 +1.1
171,228 + fhuD b0152 0.73 +1.7
171,228 + fhuD b0152 0.73 -1.8
171,238 - fhuD b0152 0.74 +2.1
171,238 - fhuD b0152 0.74 -3.6
171,307 - fhuD b0152 0.82 +2.7
171,336 + fhuD b0152 0.85 -2.0
171,336 + fhuD b0152 0.85 +2.4
171,423 + -0.5
171,423 - +2.0
171,423 - +0.4
171,506 + +0.3
171,514 - +0.1
171,533 - +2.5
171,678 + fhuB b0153 0.11 +1.1
171,678 + fhuB b0153 0.11 -2.4
171,680 + fhuB b0153 0.11 -2.0
171,680 + fhuB b0153 0.11 -1.3
171,680 - fhuB b0153 0.11 +0.5
171,680 - fhuB b0153 0.11 -1.5
171,695 + fhuB b0153 0.12 +0.6
171,742 + fhuB b0153 0.14 -0.0
171,794 + fhuB b0153 0.17 +1.2
171,867 + fhuB b0153 0.20 -2.0
171,910 + fhuB b0153 0.23 -1.2
171,910 + fhuB b0153 0.23 +0.6
172,063 + fhuB b0153 0.30 -2.0
172,088 - fhuB b0153 0.32 -2.9
172,202 + fhuB b0153 0.37 -3.0
172,218 - fhuB b0153 0.38 -2.0
172,222 - fhuB b0153 0.38 +1.2
172,261 + fhuB b0153 0.40 -1.4
172,281 + fhuB b0153 0.41 +1.1
172,281 + fhuB b0153 0.41 +2.3
172,289 + fhuB b0153 0.42 -1.3
172,290 - fhuB b0153 0.42 -1.9
172,293 + fhuB b0153 0.42 -1.2
172,293 + fhuB b0153 0.42 -2.4
172,314 + fhuB b0153 0.43 -3.1
172,369 - fhuB b0153 0.46 -1.9
172,543 - fhuB b0153 0.55 -2.7
172,543 - fhuB b0153 0.55 +0.3
172,548 + fhuB b0153 0.55 -0.1
172,569 - fhuB b0153 0.56 -3.1
172,581 + fhuB b0153 0.56 -2.1
172,687 + fhuB b0153 0.62 -1.5
172,695 - fhuB b0153 0.62 -2.8
172,723 + fhuB b0153 0.64 +1.2
172,731 - fhuB b0153 0.64 +0.6
172,734 + fhuB b0153 0.64 -0.9
172,755 + fhuB b0153 0.65 +0.3
172,767 + fhuB b0153 0.66 -0.7
172,767 + fhuB b0153 0.66 -1.1
172,823 - fhuB b0153 0.69 -2.8
172,823 - fhuB b0153 0.69 -3.1
172,922 - fhuB b0153 0.74 -1.6
172,930 + fhuB b0153 0.74 +3.4
172,946 - fhuB b0153 0.75 -1.3
172,946 - fhuB b0153 0.75 +0.4
173,030 + fhuB b0153 0.79 +0.6
173,122 - fhuB b0153 0.84 +0.1
173,122 - fhuB b0153 0.84 -2.8
173,126 - fhuB b0153 0.84 -3.4
173,209 + fhuB b0153 0.88 -0.4
173,232 - fhuB b0153 0.89 -0.6
173,241 + fhuB b0153 0.90 -2.4
173,280 + +1.0
173,280 + +1.7
173,288 - -2.0
173,288 - +1.1
173,329 + +1.4
173,378 - +0.3
173,392 + -2.5
173,401 - +2.0
173,401 - -0.9
173,463 + +0.9
173,463 - -2.5
173,468 + +0.6
173,478 + -3.0
173,600 + +0.9
173,600 - +1.5

Or see this region's nucleotide sequence