Experiment: octanol 0.08 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murC and ddlB overlap by 8 nucleotides ddlB and ftsQ are separated by 1 nucleotides ftsQ and ftsA overlap by 4 nucleotides
b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240
murC
b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153
ddlB
b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985
ftsQ
b0094: ftsA - cell division protein (NCBI), at 103,982 to 105,244
ftsA
Position (kb) 102
103
104 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 102.254 kb on + strand at 102.265 kb on + strand at 102.265 kb on + strand at 102.295 kb on + strand at 102.446 kb on + strand, within ddlB at 102.492 kb on + strand, within ddlB at 102.492 kb on + strand, within ddlB at 102.496 kb on + strand, within ddlB at 102.498 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.573 kb on + strand, within ddlB at 102.793 kb on + strand, within ddlB at 102.829 kb on + strand, within ddlB at 102.984 kb on + strand, within ddlB at 103.002 kb on + strand, within ddlB at 103.017 kb on + strand, within ddlB at 103.068 kb on + strand at 103.138 kb on + strand at 103.911 kb on + strand at 103.911 kb on + strand at 103.916 kb on + strand at 103.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction octanol 0.08 vol% remove 102,254 + -0.7 102,265 + -1.3 102,265 + -1.4 102,295 + -4.1 102,446 + ddlB b0092 0.23 -1.6 102,492 + ddlB b0092 0.28 +0.1 102,492 + ddlB b0092 0.28 +0.4 102,496 + ddlB b0092 0.29 -1.8 102,498 + ddlB b0092 0.29 -1.8 102,573 + ddlB b0092 0.37 -0.9 102,573 + ddlB b0092 0.37 -2.8 102,573 + ddlB b0092 0.37 -0.1 102,793 + ddlB b0092 0.61 -1.9 102,829 + ddlB b0092 0.65 -0.9 102,984 + ddlB b0092 0.82 -1.7 103,002 + ddlB b0092 0.83 -1.0 103,017 + ddlB b0092 0.85 -1.6 103,068 + -2.9 103,138 + -0.3 103,911 + +2.8 103,911 + -2.6 103,916 + -1.9 103,916 + +2.3
Or see this region's nucleotide sequence