Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03455 and PS417_03460 overlap by 4 nucleotides PS417_03460 and PS417_03465 are separated by 34 nucleotides PS417_03465 and PS417_03470 overlap by 4 nucleotides
PS417_03455: PS417_03455 - type III secretion protein, at 793,973 to 795,037
_03455
PS417_03460: PS417_03460 - type III secretion protein HrpI, at 795,034 to 797,127
_03460
PS417_03465: PS417_03465 - type III secretion protein, at 797,162 to 798,061
_03465
PS417_03470: PS417_03470 - ATP synthase, at 798,058 to 799,416
_03470
Position (kb) 795
796
797
798 Strain fitness (log2 ratio) -1
0
1
2 at 794.321 kb on + strand, within PS417_03455 at 794.321 kb on + strand, within PS417_03455 at 794.322 kb on - strand, within PS417_03455 at 794.817 kb on + strand at 795.181 kb on - strand at 795.181 kb on - strand at 795.218 kb on - strand at 795.218 kb on - strand at 796.000 kb on + strand, within PS417_03460 at 796.000 kb on + strand, within PS417_03460 at 796.003 kb on + strand, within PS417_03460 at 796.003 kb on + strand, within PS417_03460 at 796.004 kb on - strand, within PS417_03460 at 796.118 kb on - strand, within PS417_03460 at 796.781 kb on - strand, within PS417_03460 at 797.404 kb on - strand, within PS417_03465 at 797.521 kb on + strand, within PS417_03465 at 797.522 kb on - strand, within PS417_03465 at 798.045 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 794,321 + PS417_03455 0.33 -1.7 794,321 + PS417_03455 0.33 +0.6 794,322 - PS417_03455 0.33 +0.6 794,817 + +0.4 795,181 - -1.6 795,181 - +1.2 795,218 - +0.8 795,218 - +0.2 796,000 + PS417_03460 0.46 -0.2 796,000 + PS417_03460 0.46 +0.8 796,003 + PS417_03460 0.46 +2.2 796,003 + PS417_03460 0.46 +1.4 796,004 - PS417_03460 0.46 +0.5 796,118 - PS417_03460 0.52 +0.1 796,781 - PS417_03460 0.83 +2.0 797,404 - PS417_03465 0.27 -0.6 797,521 + PS417_03465 0.40 -0.5 797,522 - PS417_03465 0.40 -0.6 798,045 - -0.9
Or see this region's nucleotide sequence