Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24220 and PS417_24225 are separated by 3 nucleotides PS417_24225 and PS417_24230 are separated by 178 nucleotides PS417_24230 and PS417_24235 are separated by 267 nucleotides PS417_24235 and PS417_24240 are separated by 91 nucleotides
PS417_24220: PS417_24220 - hypothetical protein, at 5,252,757 to 5,254,577
_24220
PS417_24225: PS417_24225 - hypothetical protein, at 5,254,581 to 5,254,889
_24225
PS417_24230: PS417_24230 - ATP-dependent protease, at 5,255,068 to 5,257,506
_24230
PS417_24235: PS417_24235 - hypothetical protein, at 5,257,774 to 5,258,262
_24235
PS417_24240: PS417_24240 - membrane protein, at 5,258,354 to 5,260,207
_24240
Position (kb) 5255
5256
5257
5258 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.203 kb on + strand, within PS417_24220 at 5254.204 kb on - strand, within PS417_24220 at 5254.380 kb on + strand, within PS417_24220 at 5254.380 kb on + strand, within PS417_24220 at 5254.404 kb on + strand at 5254.404 kb on + strand at 5254.404 kb on + strand at 5254.404 kb on + strand at 5254.405 kb on - strand at 5254.405 kb on - strand at 5254.446 kb on + strand at 5254.473 kb on + strand at 5254.474 kb on - strand at 5254.474 kb on - strand at 5254.474 kb on - strand at 5254.474 kb on - strand at 5254.479 kb on + strand at 5254.480 kb on - strand at 5254.663 kb on + strand, within PS417_24225 at 5254.664 kb on - strand, within PS417_24225 at 5255.279 kb on + strand at 5255.279 kb on + strand at 5255.279 kb on + strand at 5255.571 kb on - strand, within PS417_24230 at 5255.625 kb on - strand, within PS417_24230 at 5255.625 kb on - strand, within PS417_24230 at 5256.044 kb on + strand, within PS417_24230 at 5256.224 kb on + strand, within PS417_24230 at 5256.228 kb on - strand, within PS417_24230 at 5256.363 kb on + strand, within PS417_24230 at 5256.474 kb on - strand, within PS417_24230 at 5256.524 kb on + strand, within PS417_24230 at 5256.678 kb on - strand, within PS417_24230 at 5256.698 kb on + strand, within PS417_24230 at 5256.726 kb on - strand, within PS417_24230 at 5257.223 kb on + strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.517 kb on + strand at 5257.518 kb on - strand at 5257.518 kb on - strand at 5257.673 kb on + strand at 5257.674 kb on - strand at 5257.699 kb on + strand at 5257.699 kb on + strand at 5257.700 kb on - strand at 5257.700 kb on - strand at 5257.703 kb on - strand at 5257.703 kb on - strand at 5257.774 kb on - strand at 5257.926 kb on + strand, within PS417_24235 at 5257.927 kb on - strand, within PS417_24235 at 5257.997 kb on - strand, within PS417_24235 at 5258.087 kb on - strand, within PS417_24235 at 5258.087 kb on - strand, within PS417_24235 at 5258.260 kb on + strand at 5258.261 kb on - strand at 5258.331 kb on + strand at 5258.342 kb on + strand at 5258.343 kb on - strand at 5258.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,254,203 + PS417_24220 0.79 -0.9 5,254,203 + PS417_24220 0.79 -1.5 5,254,203 + PS417_24220 0.79 -0.5 5,254,203 + PS417_24220 0.79 +0.3 5,254,203 + PS417_24220 0.79 +0.3 5,254,203 + PS417_24220 0.79 +0.3 5,254,203 + PS417_24220 0.79 +0.5 5,254,203 + PS417_24220 0.79 +0.4 5,254,204 - PS417_24220 0.79 -1.2 5,254,380 + PS417_24220 0.89 -1.3 5,254,380 + PS417_24220 0.89 -1.0 5,254,404 + +1.3 5,254,404 + -1.0 5,254,404 + -0.5 5,254,404 + -0.2 5,254,405 - -0.1 5,254,405 - +1.1 5,254,446 + -0.1 5,254,473 + +0.3 5,254,474 - +0.9 5,254,474 - -2.0 5,254,474 - -1.8 5,254,474 - -1.8 5,254,479 + +0.3 5,254,480 - +0.0 5,254,663 + PS417_24225 0.27 -0.6 5,254,664 - PS417_24225 0.27 -0.7 5,255,279 + -0.4 5,255,279 + -0.4 5,255,279 + -1.2 5,255,571 - PS417_24230 0.21 -0.9 5,255,625 - PS417_24230 0.23 -0.2 5,255,625 - PS417_24230 0.23 -0.5 5,256,044 + PS417_24230 0.40 +0.1 5,256,224 + PS417_24230 0.47 -2.1 5,256,228 - PS417_24230 0.48 -1.0 5,256,363 + PS417_24230 0.53 -1.4 5,256,474 - PS417_24230 0.58 -0.9 5,256,524 + PS417_24230 0.60 -1.1 5,256,678 - PS417_24230 0.66 -1.1 5,256,698 + PS417_24230 0.67 +0.3 5,256,726 - PS417_24230 0.68 -0.5 5,257,223 + PS417_24230 0.88 -2.2 5,257,224 - PS417_24230 0.88 -1.3 5,257,224 - PS417_24230 0.88 -1.0 5,257,224 - PS417_24230 0.88 +0.1 5,257,224 - PS417_24230 0.88 -1.7 5,257,224 - PS417_24230 0.88 -0.9 5,257,517 + -0.9 5,257,518 - -0.2 5,257,518 - +0.7 5,257,673 + +1.9 5,257,674 - -0.2 5,257,699 + -1.5 5,257,699 + +0.0 5,257,700 - +0.6 5,257,700 - +0.0 5,257,703 - -0.2 5,257,703 - -1.0 5,257,774 - +1.3 5,257,926 + PS417_24235 0.31 +0.7 5,257,927 - PS417_24235 0.31 +0.6 5,257,997 - PS417_24235 0.46 +1.3 5,258,087 - PS417_24235 0.64 -0.2 5,258,087 - PS417_24235 0.64 -0.7 5,258,260 + +0.4 5,258,261 - -0.4 5,258,331 + -0.8 5,258,342 + +1.6 5,258,343 - -0.6 5,258,354 - +0.3
Or see this region's nucleotide sequence