Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12930 and PS417_12935 are separated by 292 nucleotides PS417_12935 and PS417_12940 overlap by 4 nucleotides PS417_12940 and PS417_12945 are separated by 37 nucleotides PS417_12945 and PS417_12950 overlap by 4 nucleotides
PS417_12930: PS417_12930 - LpxA family transferase, at 2,827,844 to 2,828,473
_12930
PS417_12935: PS417_12935 - RNA polymerase sigma factor, at 2,828,766 to 2,829,284
_12935
PS417_12940: PS417_12940 - sugar ABC transporter substrate-binding protein, at 2,829,281 to 2,830,207
_12940
PS417_12945: PS417_12945 - sulfonate ABC transporter ATP-binding protein, at 2,830,245 to 2,830,961
_12945
PS417_12950: PS417_12950 - ABC transporter permease, at 2,830,958 to 2,831,779
_12950
Position (kb)
2829
2830
2831 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2828.465 kb on + strand at 2828.466 kb on - strand at 2828.466 kb on - strand at 2828.537 kb on + strand at 2828.606 kb on - strand at 2828.631 kb on + strand at 2828.669 kb on + strand at 2828.669 kb on + strand at 2828.780 kb on - strand at 2828.780 kb on - strand at 2828.964 kb on + strand, within PS417_12935 at 2829.031 kb on - strand, within PS417_12935 at 2829.031 kb on - strand, within PS417_12935 at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.240 kb on + strand at 2829.242 kb on + strand at 2829.242 kb on + strand at 2829.243 kb on - strand at 2829.255 kb on + strand at 2829.804 kb on + strand, within PS417_12940 at 2829.951 kb on + strand, within PS417_12940 at 2829.951 kb on + strand, within PS417_12940 at 2830.048 kb on - strand, within PS417_12940 at 2830.048 kb on - strand, within PS417_12940 at 2830.170 kb on - strand at 2830.250 kb on + strand at 2830.402 kb on + strand, within PS417_12945 at 2830.672 kb on + strand, within PS417_12945 at 2830.672 kb on + strand, within PS417_12945 at 2830.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,828,465 + +0.4 2,828,466 - -0.1 2,828,466 - +0.2 2,828,537 + -1.3 2,828,606 - -1.8 2,828,631 + -0.4 2,828,669 + +0.8 2,828,669 + +0.1 2,828,780 - +2.6 2,828,780 - +1.5 2,828,964 + PS417_12935 0.38 +0.4 2,829,031 - PS417_12935 0.51 +0.2 2,829,031 - PS417_12935 0.51 -0.3 2,829,240 + +0.1 2,829,240 + +0.5 2,829,240 + -1.7 2,829,240 + -0.9 2,829,242 + -0.5 2,829,242 + +0.2 2,829,243 - +0.1 2,829,255 + +2.5 2,829,804 + PS417_12940 0.56 -1.9 2,829,951 + PS417_12940 0.72 -2.4 2,829,951 + PS417_12940 0.72 -0.4 2,830,048 - PS417_12940 0.83 +0.0 2,830,048 - PS417_12940 0.83 -0.1 2,830,170 - +1.1 2,830,250 + +0.4 2,830,402 + PS417_12945 0.22 +0.2 2,830,672 + PS417_12945 0.60 +0.2 2,830,672 + PS417_12945 0.60 -0.5 2,830,929 + -0.3
Or see this region's nucleotide sequence