Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12455 and PS417_12460 are separated by 66 nucleotides PS417_12460 and PS417_12465 are separated by 342 nucleotides PS417_12465 and PS417_12470 are separated by 108 nucleotides
PS417_12455: PS417_12455 - hydrolase, at 2,709,313 to 2,710,137
_12455
PS417_12460: PS417_12460 - hypothetical protein, at 2,710,204 to 2,710,464
_12460
PS417_12465: PS417_12465 - Fis family transcriptional regulator, at 2,710,807 to 2,712,132
_12465
PS417_12470: PS417_12470 - hypothetical protein, at 2,712,241 to 2,713,542
_12470
Position (kb)
2710
2711
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2709.877 kb on + strand, within PS417_12455 at 2710.080 kb on + strand at 2710.081 kb on - strand at 2710.119 kb on + strand at 2710.151 kb on + strand at 2710.206 kb on - strand at 2710.206 kb on - strand at 2710.209 kb on + strand at 2710.210 kb on - strand at 2710.300 kb on - strand, within PS417_12460 at 2710.301 kb on + strand, within PS417_12460 at 2710.301 kb on + strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.302 kb on - strand, within PS417_12460 at 2710.326 kb on + strand, within PS417_12460 at 2710.528 kb on + strand at 2710.529 kb on - strand at 2710.534 kb on + strand at 2710.535 kb on - strand at 2710.698 kb on - strand at 2710.700 kb on - strand at 2710.761 kb on + strand at 2710.762 kb on - strand at 2710.973 kb on - strand at 2711.057 kb on + strand, within PS417_12465 at 2711.110 kb on - strand, within PS417_12465 at 2711.189 kb on - strand, within PS417_12465 at 2711.544 kb on - strand, within PS417_12465 at 2711.544 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.786 kb on - strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.983 kb on - strand, within PS417_12465 at 2712.249 kb on - strand at 2712.249 kb on - strand at 2712.365 kb on + strand at 2712.465 kb on + strand at 2712.493 kb on + strand, within PS417_12470 at 2712.591 kb on + strand, within PS417_12470 at 2712.599 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.859 kb on - strand, within PS417_12470 at 2712.923 kb on + strand, within PS417_12470 at 2712.987 kb on - strand, within PS417_12470 at 2713.041 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.057 kb on - strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,709,877 + PS417_12455 0.68 -0.4 2,710,080 + -0.4 2,710,081 - -0.0 2,710,119 + +0.3 2,710,151 + -0.0 2,710,206 - -0.6 2,710,206 - -0.9 2,710,209 + +0.2 2,710,210 - -0.4 2,710,300 - PS417_12460 0.37 -1.7 2,710,301 + PS417_12460 0.37 -1.7 2,710,301 + PS417_12460 0.37 -0.2 2,710,302 - PS417_12460 0.38 -1.8 2,710,302 - PS417_12460 0.38 -0.1 2,710,302 - PS417_12460 0.38 -0.1 2,710,302 - PS417_12460 0.38 +0.0 2,710,326 + PS417_12460 0.47 +0.4 2,710,528 + +0.5 2,710,529 - +0.6 2,710,534 + +0.1 2,710,535 - +1.9 2,710,698 - -0.3 2,710,700 - +1.1 2,710,761 + -0.4 2,710,762 - +0.3 2,710,973 - +0.9 2,711,057 + PS417_12465 0.19 -0.1 2,711,110 - PS417_12465 0.23 +0.0 2,711,189 - PS417_12465 0.29 -0.2 2,711,544 - PS417_12465 0.56 +0.4 2,711,544 - PS417_12465 0.56 +0.8 2,711,693 - PS417_12465 0.67 +0.3 2,711,693 - PS417_12465 0.67 +1.5 2,711,753 + PS417_12465 0.71 +0.8 2,711,753 + PS417_12465 0.71 +1.0 2,711,753 + PS417_12465 0.71 -0.7 2,711,786 - PS417_12465 0.74 +0.1 2,711,927 + PS417_12465 0.84 +1.3 2,711,927 + PS417_12465 0.84 +0.2 2,711,927 + PS417_12465 0.84 -0.4 2,711,927 + PS417_12465 0.84 +0.2 2,711,928 - PS417_12465 0.85 +0.2 2,711,928 - PS417_12465 0.85 +0.3 2,711,928 - PS417_12465 0.85 +0.6 2,711,983 - PS417_12465 0.89 +2.5 2,712,249 - -1.1 2,712,249 - -0.7 2,712,365 + -0.7 2,712,465 + +0.9 2,712,493 + PS417_12470 0.19 +0.2 2,712,591 + PS417_12470 0.27 +1.6 2,712,599 + PS417_12470 0.27 -0.1 2,712,704 + PS417_12470 0.36 -0.3 2,712,704 + PS417_12470 0.36 -1.1 2,712,705 - PS417_12470 0.36 -0.1 2,712,705 - PS417_12470 0.36 +0.4 2,712,705 - PS417_12470 0.36 -1.6 2,712,859 - PS417_12470 0.47 -1.9 2,712,923 + PS417_12470 0.52 +0.1 2,712,987 - PS417_12470 0.57 -1.1 2,713,041 + PS417_12470 0.61 +0.9 2,713,056 + PS417_12470 0.63 -0.3 2,713,056 + PS417_12470 0.63 +0.1 2,713,057 - PS417_12470 0.63 +1.2 2,713,112 + PS417_12470 0.67 -0.3 2,713,112 + PS417_12470 0.67 +0.3
Or see this region's nucleotide sequence