Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04160 and PS417_04165 overlap by 1 nucleotides PS417_04165 and PS417_04170 are separated by 145 nucleotides PS417_04170 and PS417_04175 are separated by 17 nucleotides PS417_04175 and PS417_04180 are separated by 158 nucleotides
PS417_04160: PS417_04160 - cytochrome B, at 934,000 to 935,211
_04160
PS417_04165: PS417_04165 - cytochrome C, at 935,211 to 935,990
_04165
PS417_04170: PS417_04170 - stringent starvation protein A, at 936,136 to 936,753
_04170
PS417_04175: PS417_04175 - peptidase, at 936,771 to 937,190
_04175
PS417_04180: PS417_04180 - GntR family transcriptional regulator, at 937,349 to 938,098
_04180
Position (kb) 936
937 Strain fitness (log2 ratio) -2
-1
0
1 at 935.999 kb on + strand at 936.013 kb on + strand at 936.067 kb on + strand at 936.185 kb on - strand at 936.506 kb on + strand, within PS417_04170 at 936.506 kb on + strand, within PS417_04170 at 936.507 kb on - strand, within PS417_04170 at 936.507 kb on - strand, within PS417_04170 at 936.683 kb on - strand, within PS417_04170 at 936.683 kb on - strand, within PS417_04170 at 936.758 kb on + strand at 936.789 kb on + strand at 937.035 kb on + strand, within PS417_04175 at 937.035 kb on + strand, within PS417_04175 at 937.035 kb on + strand, within PS417_04175 at 937.035 kb on + strand, within PS417_04175 at 937.036 kb on - strand, within PS417_04175 at 937.036 kb on - strand, within PS417_04175 at 937.294 kb on - strand at 937.309 kb on + strand at 937.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 935,999 + +0.4 936,013 + -0.1 936,067 + -0.2 936,185 - -1.1 936,506 + PS417_04170 0.60 -2.7 936,506 + PS417_04170 0.60 -2.7 936,507 - PS417_04170 0.60 -1.9 936,507 - PS417_04170 0.60 -2.3 936,683 - PS417_04170 0.89 -0.6 936,683 - PS417_04170 0.89 +0.1 936,758 + +0.0 936,789 + -0.7 937,035 + PS417_04175 0.63 -0.3 937,035 + PS417_04175 0.63 -0.8 937,035 + PS417_04175 0.63 -0.9 937,035 + PS417_04175 0.63 -0.9 937,036 - PS417_04175 0.63 -0.2 937,036 - PS417_04175 0.63 -1.7 937,294 - +0.2 937,309 + +0.4 937,310 - +0.8
Or see this region's nucleotide sequence