Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02995 and PS417_03000 are separated by 182 nucleotides PS417_03000 and PS417_03005 are separated by 142 nucleotides PS417_03005 and PS417_03010 are separated by 131 nucleotides
PS417_02995: PS417_02995 - NUDIX hydrolase, at 685,557 to 686,093
_02995
PS417_03000: PS417_03000 - translation initiation factor Sui1, at 686,276 to 686,647
_03000
PS417_03005: PS417_03005 - arginine decarboxylase, at 686,790 to 688,703
_03005
PS417_03010: PS417_03010 - XRE family transcriptional regulator, at 688,835 to 690,178
_03010
Position (kb) 686
687
688
689 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 685.819 kb on - strand, within PS417_02995 at 685.897 kb on - strand, within PS417_02995 at 685.928 kb on + strand, within PS417_02995 at 685.928 kb on + strand, within PS417_02995 at 685.929 kb on - strand, within PS417_02995 at 685.999 kb on + strand, within PS417_02995 at 685.999 kb on + strand, within PS417_02995 at 686.040 kb on + strand at 686.041 kb on - strand at 686.041 kb on - strand at 686.102 kb on - strand at 686.102 kb on - strand at 686.124 kb on - strand at 686.145 kb on + strand at 686.320 kb on - strand, within PS417_03000 at 686.320 kb on - strand, within PS417_03000 at 686.378 kb on - strand, within PS417_03000 at 686.378 kb on - strand, within PS417_03000 at 686.621 kb on + strand at 686.621 kb on + strand at 686.621 kb on + strand at 686.731 kb on + strand at 686.731 kb on + strand at 686.776 kb on + strand at 686.776 kb on + strand at 686.776 kb on + strand at 686.777 kb on - strand at 687.038 kb on - strand, within PS417_03005 at 687.097 kb on - strand, within PS417_03005 at 687.586 kb on - strand, within PS417_03005 at 687.687 kb on + strand, within PS417_03005 at 688.008 kb on + strand, within PS417_03005 at 688.008 kb on + strand, within PS417_03005 at 688.009 kb on - strand, within PS417_03005 at 688.083 kb on + strand, within PS417_03005 at 688.083 kb on + strand, within PS417_03005 at 688.084 kb on - strand, within PS417_03005 at 688.110 kb on - strand, within PS417_03005 at 688.182 kb on + strand, within PS417_03005 at 688.182 kb on + strand, within PS417_03005 at 688.182 kb on + strand, within PS417_03005 at 688.183 kb on - strand, within PS417_03005 at 688.339 kb on - strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.431 kb on + strand, within PS417_03005 at 688.451 kb on + strand, within PS417_03005 at 688.491 kb on + strand, within PS417_03005 at 688.574 kb on - strand at 688.702 kb on - strand at 688.702 kb on - strand at 688.829 kb on - strand at 688.840 kb on + strand at 688.840 kb on + strand at 688.841 kb on - strand at 688.843 kb on + strand at 688.843 kb on + strand at 688.844 kb on - strand at 688.847 kb on + strand at 688.868 kb on + strand at 689.059 kb on - strand, within PS417_03010 at 689.095 kb on - strand, within PS417_03010 at 689.095 kb on - strand, within PS417_03010 at 689.103 kb on + strand, within PS417_03010 at 689.104 kb on - strand, within PS417_03010 at 689.104 kb on - strand, within PS417_03010 at 689.152 kb on + strand, within PS417_03010 at 689.153 kb on - strand, within PS417_03010 at 689.330 kb on + strand, within PS417_03010 at 689.330 kb on + strand, within PS417_03010 at 689.330 kb on + strand, within PS417_03010
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 685,819 - PS417_02995 0.49 -0.9 685,897 - PS417_02995 0.63 -0.9 685,928 + PS417_02995 0.69 +2.4 685,928 + PS417_02995 0.69 -0.1 685,929 - PS417_02995 0.69 -0.6 685,999 + PS417_02995 0.82 -1.6 685,999 + PS417_02995 0.82 +0.9 686,040 + +0.1 686,041 - +1.1 686,041 - -0.2 686,102 - -0.4 686,102 - -0.6 686,124 - +1.4 686,145 + -0.1 686,320 - PS417_03000 0.12 -1.2 686,320 - PS417_03000 0.12 -0.4 686,378 - PS417_03000 0.27 -0.4 686,378 - PS417_03000 0.27 -0.3 686,621 + -1.0 686,621 + +0.1 686,621 + -0.6 686,731 + +0.1 686,731 + -1.1 686,776 + -0.5 686,776 + +0.2 686,776 + +0.3 686,777 - -1.3 687,038 - PS417_03005 0.13 -1.0 687,097 - PS417_03005 0.16 -0.9 687,586 - PS417_03005 0.42 -0.4 687,687 + PS417_03005 0.47 +1.0 688,008 + PS417_03005 0.64 -0.9 688,008 + PS417_03005 0.64 -1.6 688,009 - PS417_03005 0.64 -0.9 688,083 + PS417_03005 0.68 -0.6 688,083 + PS417_03005 0.68 -2.9 688,084 - PS417_03005 0.68 -1.0 688,110 - PS417_03005 0.69 -0.6 688,182 + PS417_03005 0.73 -2.3 688,182 + PS417_03005 0.73 -0.6 688,182 + PS417_03005 0.73 -1.2 688,183 - PS417_03005 0.73 -2.0 688,339 - PS417_03005 0.81 +0.0 688,401 + PS417_03005 0.84 -0.7 688,401 + PS417_03005 0.84 -1.7 688,401 + PS417_03005 0.84 +1.8 688,401 + PS417_03005 0.84 -1.6 688,431 + PS417_03005 0.86 -0.8 688,451 + PS417_03005 0.87 -1.7 688,491 + PS417_03005 0.89 -1.1 688,574 - -1.0 688,702 - -0.5 688,702 - -0.1 688,829 - -1.3 688,840 + +0.3 688,840 + +0.3 688,841 - -0.3 688,843 + -0.1 688,843 + +0.4 688,844 - -0.9 688,847 + -0.1 688,868 + -0.9 689,059 - PS417_03010 0.17 +0.4 689,095 - PS417_03010 0.19 +0.1 689,095 - PS417_03010 0.19 +0.0 689,103 + PS417_03010 0.20 +0.3 689,104 - PS417_03010 0.20 +1.9 689,104 - PS417_03010 0.20 -0.6 689,152 + PS417_03010 0.24 -0.6 689,153 - PS417_03010 0.24 -0.3 689,330 + PS417_03010 0.37 +0.8 689,330 + PS417_03010 0.37 +0.1 689,330 + PS417_03010 0.37 -0.1
Or see this region's nucleotide sequence