Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02770 and PS417_02775 are separated by 135 nucleotides PS417_02775 and PS417_02780 are separated by 238 nucleotides
PS417_02770: PS417_02770 - membrane protein, at 629,599 to 630,612
_02770
PS417_02775: PS417_02775 - heat-shock protein, at 630,748 to 632,016
_02775
PS417_02780: PS417_02780 - DNA-binding protein, at 632,255 to 633,199
_02780
Position (kb) 630
631
632
633 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 629.822 kb on + strand, within PS417_02770 at 629.823 kb on - strand, within PS417_02770 at 630.445 kb on + strand, within PS417_02770 at 630.807 kb on + strand at 631.268 kb on + strand, within PS417_02775 at 631.268 kb on + strand, within PS417_02775 at 631.269 kb on - strand, within PS417_02775 at 631.826 kb on + strand, within PS417_02775 at 631.827 kb on - strand, within PS417_02775 at 631.850 kb on + strand, within PS417_02775 at 631.850 kb on + strand, within PS417_02775 at 632.020 kb on + strand at 632.054 kb on + strand at 632.054 kb on + strand at 632.054 kb on + strand at 632.054 kb on + strand at 632.054 kb on + strand at 632.055 kb on - strand at 632.055 kb on - strand at 632.055 kb on - strand at 632.055 kb on - strand at 632.055 kb on - strand at 632.055 kb on - strand at 632.078 kb on - strand at 632.138 kb on + strand at 632.259 kb on - strand at 632.270 kb on + strand at 632.271 kb on - strand at 632.273 kb on + strand at 632.274 kb on - strand at 632.274 kb on - strand at 632.274 kb on - strand at 632.411 kb on + strand, within PS417_02780 at 632.411 kb on + strand, within PS417_02780 at 632.411 kb on + strand, within PS417_02780 at 632.412 kb on - strand, within PS417_02780 at 632.412 kb on - strand, within PS417_02780 at 632.633 kb on - strand, within PS417_02780
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 629,822 + PS417_02770 0.22 -0.3 629,823 - PS417_02770 0.22 +0.3 630,445 + PS417_02770 0.83 -0.3 630,807 + -0.5 631,268 + PS417_02775 0.41 -0.7 631,268 + PS417_02775 0.41 -1.0 631,269 - PS417_02775 0.41 -0.4 631,826 + PS417_02775 0.85 +0.2 631,827 - PS417_02775 0.85 -2.0 631,850 + PS417_02775 0.87 -1.2 631,850 + PS417_02775 0.87 -1.0 632,020 + -0.6 632,054 + +0.7 632,054 + -0.6 632,054 + +0.3 632,054 + -0.1 632,054 + +0.8 632,055 - +0.0 632,055 - -1.8 632,055 - -0.1 632,055 - +0.6 632,055 - +0.3 632,055 - -0.2 632,078 - +0.2 632,138 + +0.2 632,259 - +0.4 632,270 + -0.3 632,271 - +3.5 632,273 + -1.1 632,274 - +0.3 632,274 - -0.2 632,274 - +0.1 632,411 + PS417_02780 0.17 -0.6 632,411 + PS417_02780 0.17 -0.9 632,411 + PS417_02780 0.17 -0.1 632,412 - PS417_02780 0.17 -1.7 632,412 - PS417_02780 0.17 -3.3 632,633 - PS417_02780 0.40 -0.1
Or see this region's nucleotide sequence