Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02035 and PS417_02040 overlap by 4 nucleotides PS417_02040 and PS417_02045 overlap by 4 nucleotides PS417_02045 and PS417_02050 overlap by 4 nucleotides PS417_02050 and PS417_02055 overlap by 4 nucleotides
PS417_02035: PS417_02035 - 3-ketoacyl-ACP reductase, at 457,626 to 458,354
_02035
PS417_02040: PS417_02040 - 3-hydroxylacyl-ACP dehydratase, at 458,351 to 458,812
_02040
PS417_02045: PS417_02045 - 3-oxoacyl-ACP synthase, at 458,809 to 459,975
_02045
PS417_02050: PS417_02050 - hypothetical protein, at 459,972 to 460,454
_02050
PS417_02055: PS417_02055 - methyltransferase, at 460,451 to 461,185
_02055
Position (kb) 458
459
460 Strain fitness (log2 ratio) -2
-1
0
1
2 at 457.897 kb on + strand, within PS417_02035 at 457.898 kb on - strand, within PS417_02035 at 457.953 kb on - strand, within PS417_02035 at 458.009 kb on - strand, within PS417_02035 at 458.114 kb on - strand, within PS417_02035 at 458.378 kb on + strand at 458.379 kb on - strand at 458.408 kb on + strand, within PS417_02040 at 458.408 kb on + strand, within PS417_02040 at 458.409 kb on - strand, within PS417_02040 at 458.409 kb on - strand, within PS417_02040 at 458.914 kb on + strand at 458.914 kb on + strand at 458.915 kb on - strand at 459.234 kb on + strand, within PS417_02045 at 459.235 kb on - strand, within PS417_02045 at 459.599 kb on + strand, within PS417_02045 at 459.707 kb on + strand, within PS417_02045 at 459.708 kb on - strand, within PS417_02045 at 460.716 kb on + strand, within PS417_02055 at 460.716 kb on + strand, within PS417_02055 at 460.717 kb on - strand, within PS417_02055 at 460.717 kb on - strand, within PS417_02055 at 460.746 kb on + strand, within PS417_02055 at 460.747 kb on - strand, within PS417_02055
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 457,897 + PS417_02035 0.37 +0.3 457,898 - PS417_02035 0.37 -1.9 457,953 - PS417_02035 0.45 +1.1 458,009 - PS417_02035 0.53 -0.6 458,114 - PS417_02035 0.67 +0.1 458,378 + +0.3 458,379 - -1.0 458,408 + PS417_02040 0.12 -0.8 458,408 + PS417_02040 0.12 +0.1 458,409 - PS417_02040 0.13 -0.8 458,409 - PS417_02040 0.13 -0.2 458,914 + +1.1 458,914 + +1.9 458,915 - -0.4 459,234 + PS417_02045 0.36 -1.4 459,235 - PS417_02045 0.37 -2.2 459,599 + PS417_02045 0.68 -1.0 459,707 + PS417_02045 0.77 -0.4 459,708 - PS417_02045 0.77 +0.8 460,716 + PS417_02055 0.36 -0.2 460,716 + PS417_02055 0.36 -0.3 460,717 - PS417_02055 0.36 +0.6 460,717 - PS417_02055 0.36 +0.5 460,746 + PS417_02055 0.40 +1.6 460,747 - PS417_02055 0.40 +1.5
Or see this region's nucleotide sequence