Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22160 and PS417_22165 are separated by 52 nucleotides PS417_22165 and PS417_22170 are separated by 98 nucleotides PS417_22170 and PS417_22175 are separated by 5 nucleotides
PS417_22160: PS417_22160 - 3-beta hydroxysteroid dehydrogenase, at 4,801,293 to 4,802,291
_22160
PS417_22165: PS417_22165 - LysR family transcriptional regulator, at 4,802,344 to 4,803,237
_22165
PS417_22170: PS417_22170 - acetyltransferase, at 4,803,336 to 4,803,737
_22170
PS417_22175: PS417_22175 - amino acid transporter LysE, at 4,803,743 to 4,804,342
_22175
Position (kb)
4802
4803
4804 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4801.372 kb on + strand at 4801.372 kb on + strand at 4801.511 kb on - strand, within PS417_22160 at 4801.570 kb on + strand, within PS417_22160 at 4801.570 kb on + strand, within PS417_22160 at 4801.570 kb on + strand, within PS417_22160 at 4801.571 kb on - strand, within PS417_22160 at 4801.571 kb on - strand, within PS417_22160 at 4801.619 kb on - strand, within PS417_22160 at 4801.945 kb on + strand, within PS417_22160 at 4801.946 kb on - strand, within PS417_22160 at 4802.051 kb on - strand, within PS417_22160 at 4802.051 kb on - strand, within PS417_22160 at 4802.051 kb on - strand, within PS417_22160 at 4802.290 kb on + strand at 4802.293 kb on + strand at 4802.293 kb on + strand at 4802.293 kb on + strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.294 kb on - strand at 4802.297 kb on - strand at 4802.438 kb on + strand, within PS417_22165 at 4802.461 kb on - strand, within PS417_22165 at 4802.482 kb on + strand, within PS417_22165 at 4802.560 kb on - strand, within PS417_22165 at 4802.581 kb on + strand, within PS417_22165 at 4802.582 kb on - strand at 4802.582 kb on - strand, within PS417_22165 at 4802.624 kb on + strand, within PS417_22165 at 4803.226 kb on - strand at 4803.280 kb on - strand at 4803.321 kb on + strand at 4803.322 kb on - strand at 4803.323 kb on + strand at 4803.324 kb on - strand at 4803.324 kb on - strand at 4803.402 kb on + strand, within PS417_22170 at 4803.402 kb on + strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.654 kb on + strand at 4803.706 kb on + strand at 4803.706 kb on + strand at 4803.707 kb on - strand at 4803.736 kb on - strand at 4803.742 kb on + strand at 4803.743 kb on - strand at 4803.820 kb on - strand, within PS417_22175 at 4804.178 kb on + strand, within PS417_22175
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,801,372 + -0.6 4,801,372 + -0.3 4,801,511 - PS417_22160 0.22 -2.5 4,801,570 + PS417_22160 0.28 -0.3 4,801,570 + PS417_22160 0.28 -0.5 4,801,570 + PS417_22160 0.28 -0.6 4,801,571 - PS417_22160 0.28 +0.3 4,801,571 - PS417_22160 0.28 -0.0 4,801,619 - PS417_22160 0.33 +1.0 4,801,945 + PS417_22160 0.65 +0.2 4,801,946 - PS417_22160 0.65 -0.8 4,802,051 - PS417_22160 0.76 -0.2 4,802,051 - PS417_22160 0.76 -0.1 4,802,051 - PS417_22160 0.76 -3.0 4,802,290 + -0.3 4,802,293 + -0.6 4,802,293 + +0.7 4,802,293 + -0.1 4,802,294 - +1.5 4,802,294 - -0.0 4,802,294 - -2.7 4,802,294 - +0.1 4,802,294 - +0.3 4,802,294 - +0.3 4,802,294 - -0.2 4,802,294 - -2.3 4,802,297 - +0.5 4,802,438 + PS417_22165 0.11 -1.0 4,802,461 - PS417_22165 0.13 -1.5 4,802,482 + PS417_22165 0.15 -0.1 4,802,560 - PS417_22165 0.24 -1.1 4,802,581 + PS417_22165 0.27 -0.7 4,802,582 - +2.5 4,802,582 - PS417_22165 0.27 -0.4 4,802,624 + PS417_22165 0.31 -0.9 4,803,226 - +0.0 4,803,280 - -0.4 4,803,321 + -1.5 4,803,322 - -0.3 4,803,323 + +1.8 4,803,324 - -2.9 4,803,324 - -1.2 4,803,402 + PS417_22170 0.16 -0.7 4,803,402 + PS417_22170 0.16 +0.5 4,803,403 - PS417_22170 0.17 -0.1 4,803,403 - PS417_22170 0.17 -1.0 4,803,654 + +1.5 4,803,706 + -1.3 4,803,706 + -1.1 4,803,707 - +0.4 4,803,736 - -0.0 4,803,742 + -0.3 4,803,743 - -1.3 4,803,820 - PS417_22175 0.13 +0.9 4,804,178 + PS417_22175 0.72 +2.1
Or see this region's nucleotide sequence