Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16220 and PS417_16225 are separated by 10 nucleotides PS417_16225 and PS417_16230 are separated by 10 nucleotides PS417_16230 and PS417_16235 overlap by 4 nucleotides
PS417_16220: PS417_16220 - membrane protein, at 3,555,324 to 3,555,596
_16220
PS417_16225: PS417_16225 - sugar ABC transporter permease, at 3,555,607 to 3,556,407
_16225
PS417_16230: PS417_16230 - ABC transporter permease, at 3,556,418 to 3,557,284
_16230
PS417_16235: PS417_16235 - ABC transporter ATP-binding protein, at 3,557,281 to 3,558,378
_16235
Position (kb)
3556
3557
3558 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3555.422 kb on - strand, within PS417_16220 at 3555.681 kb on + strand at 3555.681 kb on + strand at 3556.043 kb on + strand, within PS417_16225 at 3556.043 kb on + strand, within PS417_16225 at 3556.044 kb on - strand, within PS417_16225 at 3556.198 kb on + strand, within PS417_16225 at 3556.198 kb on + strand, within PS417_16225 at 3556.198 kb on + strand, within PS417_16225 at 3556.199 kb on - strand, within PS417_16225 at 3556.199 kb on - strand, within PS417_16225 at 3556.202 kb on + strand, within PS417_16225 at 3556.202 kb on + strand, within PS417_16225 at 3556.203 kb on - strand, within PS417_16225 at 3556.205 kb on + strand, within PS417_16225 at 3556.230 kb on - strand at 3556.259 kb on + strand, within PS417_16225 at 3556.373 kb on + strand at 3556.373 kb on + strand at 3556.373 kb on + strand at 3556.374 kb on - strand at 3556.374 kb on - strand at 3556.374 kb on - strand at 3556.402 kb on - strand at 3556.488 kb on + strand at 3556.489 kb on - strand at 3556.532 kb on + strand at 3556.567 kb on + strand, within PS417_16230 at 3556.567 kb on + strand, within PS417_16230 at 3556.568 kb on - strand, within PS417_16230 at 3556.571 kb on - strand, within PS417_16230 at 3556.614 kb on + strand, within PS417_16230 at 3556.634 kb on - strand, within PS417_16230 at 3556.688 kb on - strand, within PS417_16230 at 3556.688 kb on - strand, within PS417_16230 at 3556.688 kb on - strand, within PS417_16230 at 3556.740 kb on + strand, within PS417_16230 at 3556.741 kb on - strand, within PS417_16230 at 3556.805 kb on + strand, within PS417_16230 at 3556.858 kb on + strand, within PS417_16230 at 3556.861 kb on - strand, within PS417_16230 at 3556.887 kb on + strand, within PS417_16230 at 3557.125 kb on + strand, within PS417_16230 at 3557.126 kb on - strand, within PS417_16230 at 3557.127 kb on + strand, within PS417_16230 at 3557.127 kb on + strand, within PS417_16230 at 3557.128 kb on - strand, within PS417_16230 at 3557.148 kb on + strand, within PS417_16230 at 3557.184 kb on + strand, within PS417_16230 at 3557.185 kb on - strand, within PS417_16230 at 3557.303 kb on + strand at 3557.316 kb on - strand at 3557.424 kb on + strand, within PS417_16235 at 3557.432 kb on + strand, within PS417_16235 at 3557.432 kb on + strand at 3557.486 kb on + strand, within PS417_16235 at 3557.556 kb on + strand, within PS417_16235 at 3557.557 kb on - strand, within PS417_16235 at 3557.676 kb on + strand, within PS417_16235 at 3557.676 kb on + strand, within PS417_16235 at 3557.677 kb on - strand, within PS417_16235 at 3557.735 kb on + strand, within PS417_16235 at 3557.736 kb on - strand, within PS417_16235 at 3557.736 kb on - strand, within PS417_16235 at 3557.736 kb on - strand, within PS417_16235 at 3557.736 kb on - strand, within PS417_16235 at 3557.792 kb on + strand, within PS417_16235 at 3557.792 kb on + strand, within PS417_16235 at 3557.792 kb on + strand, within PS417_16235 at 3557.793 kb on - strand, within PS417_16235 at 3558.102 kb on - strand, within PS417_16235
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,555,422 - PS417_16220 0.36 +0.8 3,555,681 + +0.3 3,555,681 + +0.2 3,556,043 + PS417_16225 0.54 -0.1 3,556,043 + PS417_16225 0.54 +1.0 3,556,044 - PS417_16225 0.55 +0.1 3,556,198 + PS417_16225 0.74 -0.9 3,556,198 + PS417_16225 0.74 +1.2 3,556,198 + PS417_16225 0.74 +0.2 3,556,199 - PS417_16225 0.74 -0.8 3,556,199 - PS417_16225 0.74 +0.2 3,556,202 + PS417_16225 0.74 -1.8 3,556,202 + PS417_16225 0.74 -0.5 3,556,203 - PS417_16225 0.74 +0.7 3,556,205 + PS417_16225 0.75 -0.1 3,556,230 - +1.7 3,556,259 + PS417_16225 0.81 -0.3 3,556,373 + -0.4 3,556,373 + -0.7 3,556,373 + -0.6 3,556,374 - -0.7 3,556,374 - +0.4 3,556,374 - +0.2 3,556,402 - +0.2 3,556,488 + -0.9 3,556,489 - +0.7 3,556,532 + +1.2 3,556,567 + PS417_16230 0.17 -0.6 3,556,567 + PS417_16230 0.17 -0.6 3,556,568 - PS417_16230 0.17 +0.9 3,556,571 - PS417_16230 0.18 +1.2 3,556,614 + PS417_16230 0.23 -0.2 3,556,634 - PS417_16230 0.25 +4.5 3,556,688 - PS417_16230 0.31 +0.0 3,556,688 - PS417_16230 0.31 +0.6 3,556,688 - PS417_16230 0.31 +1.0 3,556,740 + PS417_16230 0.37 +0.9 3,556,741 - PS417_16230 0.37 -1.6 3,556,805 + PS417_16230 0.45 +0.7 3,556,858 + PS417_16230 0.51 -0.1 3,556,861 - PS417_16230 0.51 -0.4 3,556,887 + PS417_16230 0.54 +0.7 3,557,125 + PS417_16230 0.82 +0.8 3,557,126 - PS417_16230 0.82 +0.7 3,557,127 + PS417_16230 0.82 -0.8 3,557,127 + PS417_16230 0.82 +1.3 3,557,128 - PS417_16230 0.82 +0.9 3,557,148 + PS417_16230 0.84 +1.5 3,557,184 + PS417_16230 0.88 +0.1 3,557,185 - PS417_16230 0.88 -0.6 3,557,303 + -0.4 3,557,316 - -1.6 3,557,424 + PS417_16235 0.13 +0.1 3,557,432 + PS417_16235 0.14 +0.8 3,557,432 + +1.2 3,557,486 + PS417_16235 0.19 -0.2 3,557,556 + PS417_16235 0.25 +0.4 3,557,557 - PS417_16235 0.25 +2.3 3,557,676 + PS417_16235 0.36 -0.0 3,557,676 + PS417_16235 0.36 -1.6 3,557,677 - PS417_16235 0.36 -1.3 3,557,735 + PS417_16235 0.41 -1.0 3,557,736 - PS417_16235 0.41 -0.1 3,557,736 - PS417_16235 0.41 +0.6 3,557,736 - PS417_16235 0.41 -0.9 3,557,736 - PS417_16235 0.41 +1.0 3,557,792 + PS417_16235 0.47 -1.0 3,557,792 + PS417_16235 0.47 +1.2 3,557,792 + PS417_16235 0.47 +0.7 3,557,793 - PS417_16235 0.47 +1.6 3,558,102 - PS417_16235 0.75 +0.1
Or see this region's nucleotide sequence