Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10350 and PS417_10355 are separated by 7 nucleotides PS417_10355 and PS417_10360 are separated by 90 nucleotides
PS417_10350: PS417_10350 - multidrug MFS transporter, at 2,212,250 to 2,213,542
_10350
PS417_10355: PS417_10355 - transcriptional regulator, at 2,213,550 to 2,216,420
_10355
PS417_10360: PS417_10360 - MFS transporter, at 2,216,511 to 2,217,725
_10360
Position (kb)
2213
2214
2215
2216
2217 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2212.850 kb on + strand, within PS417_10350 at 2212.859 kb on + strand, within PS417_10350 at 2212.859 kb on + strand, within PS417_10350 at 2212.859 kb on + strand, within PS417_10350 at 2212.859 kb on + strand, within PS417_10350 at 2212.860 kb on - strand, within PS417_10350 at 2212.860 kb on - strand, within PS417_10350 at 2213.040 kb on - strand, within PS417_10350 at 2213.247 kb on - strand, within PS417_10350 at 2213.306 kb on - strand, within PS417_10350 at 2213.306 kb on - strand, within PS417_10350 at 2213.306 kb on - strand, within PS417_10350 at 2213.306 kb on - strand, within PS417_10350 at 2213.429 kb on + strand at 2213.429 kb on + strand at 2213.430 kb on - strand at 2213.432 kb on + strand at 2213.433 kb on - strand at 2213.433 kb on - strand at 2213.433 kb on - strand at 2213.434 kb on + strand at 2213.647 kb on + strand at 2213.647 kb on + strand at 2214.082 kb on + strand, within PS417_10355 at 2214.083 kb on - strand, within PS417_10355 at 2214.167 kb on - strand, within PS417_10355 at 2214.200 kb on - strand, within PS417_10355 at 2214.344 kb on + strand, within PS417_10355 at 2214.427 kb on + strand, within PS417_10355 at 2214.527 kb on + strand, within PS417_10355 at 2214.528 kb on - strand, within PS417_10355 at 2214.547 kb on + strand, within PS417_10355 at 2214.694 kb on - strand, within PS417_10355 at 2214.722 kb on - strand, within PS417_10355 at 2215.203 kb on + strand, within PS417_10355 at 2215.253 kb on - strand at 2215.359 kb on + strand, within PS417_10355 at 2215.359 kb on + strand at 2215.359 kb on + strand, within PS417_10355 at 2215.359 kb on + strand, within PS417_10355 at 2215.360 kb on - strand at 2216.126 kb on + strand, within PS417_10355 at 2216.126 kb on + strand, within PS417_10355 at 2216.451 kb on + strand at 2216.491 kb on + strand at 2216.633 kb on - strand, within PS417_10360 at 2216.633 kb on - strand, within PS417_10360 at 2216.633 kb on - strand, within PS417_10360 at 2216.815 kb on + strand, within PS417_10360 at 2216.816 kb on - strand at 2216.816 kb on - strand, within PS417_10360 at 2217.074 kb on - strand, within PS417_10360 at 2217.220 kb on - strand, within PS417_10360 at 2217.301 kb on - strand, within PS417_10360
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,212,850 + PS417_10350 0.46 +2.1 2,212,859 + PS417_10350 0.47 +0.8 2,212,859 + PS417_10350 0.47 +0.0 2,212,859 + PS417_10350 0.47 +0.5 2,212,859 + PS417_10350 0.47 -0.5 2,212,860 - PS417_10350 0.47 +1.6 2,212,860 - PS417_10350 0.47 -2.0 2,213,040 - PS417_10350 0.61 -0.8 2,213,247 - PS417_10350 0.77 -0.1 2,213,306 - PS417_10350 0.82 +0.2 2,213,306 - PS417_10350 0.82 +0.1 2,213,306 - PS417_10350 0.82 -0.6 2,213,306 - PS417_10350 0.82 +0.4 2,213,429 + -0.1 2,213,429 + +0.5 2,213,430 - +0.7 2,213,432 + +0.2 2,213,433 - -0.4 2,213,433 - -0.2 2,213,433 - +1.2 2,213,434 + -0.5 2,213,647 + -0.0 2,213,647 + -0.2 2,214,082 + PS417_10355 0.19 +0.2 2,214,083 - PS417_10355 0.19 -0.2 2,214,167 - PS417_10355 0.21 -1.2 2,214,200 - PS417_10355 0.23 +0.2 2,214,344 + PS417_10355 0.28 -0.8 2,214,427 + PS417_10355 0.31 -0.4 2,214,527 + PS417_10355 0.34 +0.4 2,214,528 - PS417_10355 0.34 -0.5 2,214,547 + PS417_10355 0.35 -1.4 2,214,694 - PS417_10355 0.40 -0.9 2,214,722 - PS417_10355 0.41 -0.8 2,215,203 + PS417_10355 0.58 -0.4 2,215,253 - +1.2 2,215,359 + PS417_10355 0.63 -0.3 2,215,359 + +2.8 2,215,359 + PS417_10355 0.63 -0.7 2,215,359 + PS417_10355 0.63 -1.3 2,215,360 - -0.1 2,216,126 + PS417_10355 0.90 -0.4 2,216,126 + PS417_10355 0.90 -0.1 2,216,451 + +0.8 2,216,491 + -1.1 2,216,633 - PS417_10360 0.10 -0.6 2,216,633 - PS417_10360 0.10 +0.7 2,216,633 - PS417_10360 0.10 +0.6 2,216,815 + PS417_10360 0.25 -1.9 2,216,816 - +0.2 2,216,816 - PS417_10360 0.25 -1.1 2,217,074 - PS417_10360 0.46 -0.2 2,217,220 - PS417_10360 0.58 +0.1 2,217,301 - PS417_10360 0.65 -0.1
Or see this region's nucleotide sequence