Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05020 and PS417_05025 are separated by 12 nucleotides PS417_05025 and PS417_05030 are separated by 39 nucleotides PS417_05030 and PS417_05035 overlap by 4 nucleotides PS417_05035 and PS417_05040 are separated by 187 nucleotides
PS417_05020: PS417_05020 - fumarylacetoacetase, at 1,116,755 to 1,118,041
_05020
PS417_05025: PS417_05025 - maleylacetoacetate isomerase, at 1,118,054 to 1,118,695
_05025
PS417_05030: PS417_05030 - major facilitator transporter, at 1,118,735 to 1,120,102
_05030
PS417_05035: PS417_05035 - hypothetical protein, at 1,120,099 to 1,120,902
_05035
PS417_05040: PS417_05040 - amino acid dehydrogenase, at 1,121,090 to 1,122,109
_05040
Position (kb) 1118
1119
1120
1121 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1117.754 kb on + strand, within PS417_05020 at 1118.040 kb on - strand at 1118.069 kb on + strand at 1118.070 kb on - strand at 1118.542 kb on + strand, within PS417_05025 at 1118.564 kb on + strand, within PS417_05025 at 1118.727 kb on + strand at 1118.844 kb on - strand at 1118.864 kb on + strand at 1118.865 kb on - strand at 1118.948 kb on - strand, within PS417_05030 at 1119.027 kb on - strand, within PS417_05030 at 1119.027 kb on - strand, within PS417_05030 at 1119.091 kb on + strand, within PS417_05030 at 1119.091 kb on + strand, within PS417_05030 at 1119.169 kb on + strand, within PS417_05030 at 1119.170 kb on - strand, within PS417_05030 at 1119.170 kb on - strand, within PS417_05030 at 1119.369 kb on - strand, within PS417_05030 at 1119.495 kb on - strand, within PS417_05030 at 1119.551 kb on + strand, within PS417_05030 at 1119.552 kb on - strand, within PS417_05030 at 1119.776 kb on + strand, within PS417_05030 at 1119.777 kb on - strand, within PS417_05030 at 1119.870 kb on + strand, within PS417_05030 at 1119.870 kb on + strand, within PS417_05030 at 1119.871 kb on - strand, within PS417_05030 at 1119.871 kb on - strand, within PS417_05030 at 1120.492 kb on + strand, within PS417_05035 at 1120.492 kb on + strand, within PS417_05035 at 1120.493 kb on - strand, within PS417_05035 at 1120.804 kb on + strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.805 kb on - strand, within PS417_05035 at 1120.981 kb on + strand at 1120.981 kb on + strand at 1120.981 kb on + strand at 1120.982 kb on - strand at 1121.012 kb on + strand at 1121.013 kb on - strand at 1121.049 kb on + strand at 1121.050 kb on - strand at 1121.050 kb on - strand at 1121.051 kb on + strand at 1121.051 kb on + strand at 1121.052 kb on - strand at 1121.052 kb on - strand at 1121.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,117,754 + PS417_05020 0.78 -0.0 1,118,040 - +0.5 1,118,069 + +1.1 1,118,070 - -0.2 1,118,542 + PS417_05025 0.76 +0.2 1,118,564 + PS417_05025 0.79 -0.1 1,118,727 + +0.8 1,118,844 - -1.6 1,118,864 + +0.1 1,118,865 - +0.2 1,118,948 - PS417_05030 0.16 +0.5 1,119,027 - PS417_05030 0.21 +0.7 1,119,027 - PS417_05030 0.21 +1.0 1,119,091 + PS417_05030 0.26 -0.0 1,119,091 + PS417_05030 0.26 +1.8 1,119,169 + PS417_05030 0.32 -0.4 1,119,170 - PS417_05030 0.32 -1.9 1,119,170 - PS417_05030 0.32 -0.0 1,119,369 - PS417_05030 0.46 +1.3 1,119,495 - PS417_05030 0.56 -1.4 1,119,551 + PS417_05030 0.60 +1.0 1,119,552 - PS417_05030 0.60 +1.0 1,119,776 + PS417_05030 0.76 +0.8 1,119,777 - PS417_05030 0.76 -0.1 1,119,870 + PS417_05030 0.83 +0.3 1,119,870 + PS417_05030 0.83 +0.8 1,119,871 - PS417_05030 0.83 -1.0 1,119,871 - PS417_05030 0.83 +0.8 1,120,492 + PS417_05035 0.49 +0.0 1,120,492 + PS417_05035 0.49 -0.2 1,120,493 - PS417_05035 0.49 +0.1 1,120,804 + PS417_05035 0.88 -0.0 1,120,805 - PS417_05035 0.88 -0.8 1,120,805 - PS417_05035 0.88 -2.2 1,120,805 - PS417_05035 0.88 -1.0 1,120,981 + +0.1 1,120,981 + -0.6 1,120,981 + +1.2 1,120,982 - +0.0 1,121,012 + -0.2 1,121,013 - -0.6 1,121,049 + -0.5 1,121,050 - +0.1 1,121,050 - -1.1 1,121,051 + -1.1 1,121,051 + -1.4 1,121,052 - -1.0 1,121,052 - +1.7 1,121,052 - +0.7
Or see this region's nucleotide sequence