Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26950 and PS417_26955 are separated by 16 nucleotides PS417_26955 and PS417_26960 are separated by 85 nucleotides PS417_26960 and PS417_26965 are separated by 144 nucleotides
PS417_26950: PS417_26950 - LysR family transcriptional regulator, at 5,820,497 to 5,821,360
_26950
PS417_26955: PS417_26955 - ATPase P, at 5,821,377 to 5,823,623
_26955
PS417_26960: PS417_26960 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 5,823,709 to 5,824,149
_26960
PS417_26965: PS417_26965 - thymidylate synthase, at 5,824,294 to 5,825,127
_26965
Position (kb) 5821
5822
5823
5824 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5820.498 kb on + strand at 5820.498 kb on + strand at 5820.499 kb on - strand at 5820.499 kb on - strand at 5820.499 kb on - strand at 5820.502 kb on + strand at 5820.502 kb on + strand at 5820.502 kb on + strand at 5820.502 kb on + strand at 5820.503 kb on - strand at 5820.503 kb on - strand at 5820.503 kb on - strand at 5820.503 kb on - strand at 5820.503 kb on - strand at 5820.573 kb on + strand at 5820.830 kb on + strand, within PS417_26950 at 5820.831 kb on - strand, within PS417_26950 at 5820.831 kb on - strand, within PS417_26950 at 5821.059 kb on + strand, within PS417_26950 at 5821.059 kb on + strand, within PS417_26950 at 5821.059 kb on + strand, within PS417_26950 at 5821.059 kb on + strand, within PS417_26950 at 5821.059 kb on + strand, within PS417_26950 at 5821.059 kb on + strand, within PS417_26950 at 5821.060 kb on - strand, within PS417_26950 at 5821.062 kb on + strand, within PS417_26950 at 5821.062 kb on + strand, within PS417_26950 at 5821.063 kb on - strand, within PS417_26950 at 5821.063 kb on - strand, within PS417_26950 at 5821.063 kb on - strand, within PS417_26950 at 5821.063 kb on - strand, within PS417_26950 at 5821.063 kb on - strand, within PS417_26950 at 5821.162 kb on + strand, within PS417_26950 at 5821.162 kb on + strand, within PS417_26950 at 5821.343 kb on + strand at 5823.763 kb on + strand, within PS417_26960 at 5824.147 kb on + strand at 5824.148 kb on - strand at 5824.231 kb on + strand at 5824.231 kb on + strand at 5824.232 kb on - strand at 5824.232 kb on - strand at 5824.244 kb on + strand at 5824.244 kb on + strand at 5824.244 kb on + strand at 5824.244 kb on + strand at 5824.244 kb on + strand at 5824.244 kb on + strand at 5824.244 kb on + strand at 5824.245 kb on - strand at 5824.245 kb on - strand at 5824.245 kb on - strand at 5824.245 kb on - strand at 5824.245 kb on - strand at 5824.245 kb on - strand at 5824.245 kb on - strand at 5824.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,820,498 + -0.4 5,820,498 + +0.2 5,820,499 - -0.6 5,820,499 - +1.2 5,820,499 - -1.3 5,820,502 + +1.2 5,820,502 + +0.8 5,820,502 + +0.2 5,820,502 + -1.8 5,820,503 - +0.8 5,820,503 - -0.4 5,820,503 - +0.5 5,820,503 - -0.2 5,820,503 - +0.9 5,820,573 + +0.2 5,820,830 + PS417_26950 0.39 -0.4 5,820,831 - PS417_26950 0.39 +0.8 5,820,831 - PS417_26950 0.39 +1.0 5,821,059 + PS417_26950 0.65 +1.3 5,821,059 + PS417_26950 0.65 -2.4 5,821,059 + PS417_26950 0.65 +1.5 5,821,059 + PS417_26950 0.65 -1.0 5,821,059 + PS417_26950 0.65 +1.5 5,821,059 + PS417_26950 0.65 -0.0 5,821,060 - PS417_26950 0.65 +0.9 5,821,062 + PS417_26950 0.65 +1.0 5,821,062 + PS417_26950 0.65 +0.7 5,821,063 - PS417_26950 0.66 -1.3 5,821,063 - PS417_26950 0.66 +1.7 5,821,063 - PS417_26950 0.66 -0.4 5,821,063 - PS417_26950 0.66 -0.4 5,821,063 - PS417_26950 0.66 -0.2 5,821,162 + PS417_26950 0.77 +0.3 5,821,162 + PS417_26950 0.77 +0.0 5,821,343 + +0.1 5,823,763 + PS417_26960 0.12 -1.0 5,824,147 + -1.3 5,824,148 - -4.1 5,824,231 + +2.2 5,824,231 + -2.9 5,824,232 - -0.8 5,824,232 - -0.5 5,824,244 + +1.6 5,824,244 + -1.1 5,824,244 + +0.1 5,824,244 + -0.0 5,824,244 + -0.1 5,824,244 + +0.8 5,824,244 + -0.3 5,824,245 - -1.0 5,824,245 - -1.4 5,824,245 - -0.2 5,824,245 - -3.0 5,824,245 - +0.4 5,824,245 - +0.9 5,824,245 - +2.1 5,824,279 - -0.1
Or see this region's nucleotide sequence