Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24305 and PS417_24310 are separated by 35 nucleotides PS417_24310 and PS417_24315 are separated by 58 nucleotides PS417_24315 and PS417_24320 are separated by 287 nucleotides PS417_24320 and PS417_24325 are separated by 45 nucleotides
PS417_24305: PS417_24305 - DNA repair protein RadA, at 5,275,394 to 5,276,761
_24305
PS417_24310: PS417_24310 - ankyrin, at 5,276,797 to 5,277,336
_24310
PS417_24315: PS417_24315 - catalase, at 5,277,395 to 5,278,936
_24315
PS417_24320: PS417_24320 - large-conductance mechanosensitive channel, at 5,279,224 to 5,279,643
_24320
PS417_24325: PS417_24325 - ferredoxin-NADP reductase, at 5,279,689 to 5,280,465
_24325
Position (kb)
5277
5278
5279 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5276.395 kb on - strand, within PS417_24305 at 5276.435 kb on + strand, within PS417_24305 at 5276.436 kb on - strand, within PS417_24305 at 5276.689 kb on + strand at 5276.739 kb on + strand at 5276.739 kb on + strand at 5276.739 kb on + strand at 5276.739 kb on + strand at 5276.739 kb on + strand at 5276.793 kb on + strand at 5276.794 kb on - strand at 5276.794 kb on - strand at 5276.934 kb on - strand, within PS417_24310 at 5277.146 kb on + strand, within PS417_24310 at 5277.146 kb on + strand, within PS417_24310 at 5277.176 kb on + strand, within PS417_24310 at 5277.472 kb on - strand at 5277.489 kb on + strand at 5277.489 kb on + strand at 5277.723 kb on + strand, within PS417_24315 at 5277.724 kb on - strand, within PS417_24315 at 5277.838 kb on - strand, within PS417_24315 at 5278.083 kb on + strand, within PS417_24315 at 5278.083 kb on + strand, within PS417_24315 at 5278.083 kb on + strand, within PS417_24315 at 5278.084 kb on - strand, within PS417_24315 at 5278.084 kb on - strand, within PS417_24315 at 5278.512 kb on + strand, within PS417_24315 at 5278.698 kb on - strand, within PS417_24315 at 5279.012 kb on - strand at 5279.012 kb on - strand at 5279.015 kb on + strand at 5279.042 kb on - strand at 5279.108 kb on - strand at 5279.118 kb on + strand at 5279.145 kb on - strand at 5279.181 kb on + strand at 5279.182 kb on - strand at 5279.209 kb on - strand at 5279.224 kb on - strand at 5279.265 kb on + strand at 5279.265 kb on + strand at 5279.266 kb on - strand, within PS417_24320 at 5279.266 kb on - strand, within PS417_24320 at 5279.287 kb on + strand, within PS417_24320 at 5279.293 kb on - strand, within PS417_24320 at 5279.333 kb on + strand, within PS417_24320 at 5279.334 kb on - strand, within PS417_24320 at 5279.401 kb on - strand, within PS417_24320 at 5279.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,276,395 - PS417_24305 0.73 -0.6 5,276,435 + PS417_24305 0.76 -0.5 5,276,436 - PS417_24305 0.76 +0.4 5,276,689 + +1.5 5,276,739 + -1.8 5,276,739 + -1.0 5,276,739 + -0.7 5,276,739 + -0.1 5,276,739 + -1.0 5,276,793 + -1.3 5,276,794 - +0.5 5,276,794 - -0.3 5,276,934 - PS417_24310 0.25 -1.4 5,277,146 + PS417_24310 0.65 -1.5 5,277,146 + PS417_24310 0.65 +1.0 5,277,176 + PS417_24310 0.70 +0.7 5,277,472 - +1.2 5,277,489 + -1.0 5,277,489 + +0.6 5,277,723 + PS417_24315 0.21 +0.7 5,277,724 - PS417_24315 0.21 -0.2 5,277,838 - PS417_24315 0.29 -1.0 5,278,083 + PS417_24315 0.45 +2.4 5,278,083 + PS417_24315 0.45 +1.1 5,278,083 + PS417_24315 0.45 +1.1 5,278,084 - PS417_24315 0.45 +0.3 5,278,084 - PS417_24315 0.45 +0.1 5,278,512 + PS417_24315 0.72 +0.5 5,278,698 - PS417_24315 0.85 +0.5 5,279,012 - +1.4 5,279,012 - -0.2 5,279,015 + +0.0 5,279,042 - +1.3 5,279,108 - +0.0 5,279,118 + -1.0 5,279,145 - +0.1 5,279,181 + -1.0 5,279,182 - +0.6 5,279,209 - -1.0 5,279,224 - +0.7 5,279,265 + +0.6 5,279,265 + +0.7 5,279,266 - PS417_24320 0.10 -0.1 5,279,266 - PS417_24320 0.10 +0.0 5,279,287 + PS417_24320 0.15 -0.4 5,279,293 - PS417_24320 0.16 +1.0 5,279,333 + PS417_24320 0.26 +0.2 5,279,334 - PS417_24320 0.26 -0.1 5,279,401 - PS417_24320 0.42 -1.7 5,279,696 + -0.5
Or see this region's nucleotide sequence