Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22235 and PS417_22240 are separated by 638 nucleotides PS417_22240 and PS417_22245 are separated by 73 nucleotides PS417_22245 and PS417_22250 are separated by 196 nucleotides
PS417_22235: PS417_22235 - aromatic amino acid transporter, at 4,818,922 to 4,820,343
_22235
PS417_22240: PS417_22240 - mercuric reductase, at 4,820,982 to 4,821,119
_22240
PS417_22245: PS417_22245 - leucyl aminopeptidase (aminopeptidase T), at 4,821,193 to 4,822,152
_22245
PS417_22250: PS417_22250 - pseudouridine synthase, at 4,822,349 to 4,823,560
_22250
Position (kb)
4821
4822
4823 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4820.220 kb on + strand at 4820.220 kb on + strand at 4820.286 kb on + strand at 4820.446 kb on + strand at 4820.446 kb on + strand at 4820.446 kb on + strand at 4820.695 kb on + strand at 4820.741 kb on + strand at 4820.911 kb on - strand at 4820.976 kb on + strand at 4820.977 kb on - strand at 4821.118 kb on - strand at 4821.226 kb on + strand at 4821.227 kb on - strand at 4821.230 kb on - strand at 4821.441 kb on + strand, within PS417_22245 at 4821.442 kb on - strand, within PS417_22245 at 4821.539 kb on - strand, within PS417_22245 at 4821.547 kb on + strand, within PS417_22245 at 4821.553 kb on + strand, within PS417_22245 at 4821.553 kb on + strand, within PS417_22245 at 4821.554 kb on - strand, within PS417_22245 at 4821.554 kb on - strand, within PS417_22245 at 4821.823 kb on + strand, within PS417_22245 at 4821.824 kb on - strand, within PS417_22245 at 4821.922 kb on + strand, within PS417_22245 at 4821.923 kb on - strand, within PS417_22245 at 4821.997 kb on + strand, within PS417_22245 at 4821.998 kb on - strand, within PS417_22245 at 4821.998 kb on - strand, within PS417_22245 at 4822.151 kb on - strand at 4822.151 kb on - strand at 4822.181 kb on - strand at 4822.223 kb on + strand at 4822.528 kb on + strand, within PS417_22250 at 4822.636 kb on + strand, within PS417_22250 at 4822.637 kb on - strand, within PS417_22250
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,820,220 + -0.9 4,820,220 + +0.7 4,820,286 + -1.3 4,820,446 + -1.2 4,820,446 + +0.0 4,820,446 + +1.2 4,820,695 + +0.1 4,820,741 + -0.4 4,820,911 - +0.3 4,820,976 + +1.2 4,820,977 - +0.4 4,821,118 - -0.1 4,821,226 + +0.2 4,821,227 - -0.2 4,821,230 - -0.6 4,821,441 + PS417_22245 0.26 -0.6 4,821,442 - PS417_22245 0.26 +1.3 4,821,539 - PS417_22245 0.36 +2.0 4,821,547 + PS417_22245 0.37 -0.3 4,821,553 + PS417_22245 0.38 +0.5 4,821,553 + PS417_22245 0.38 -0.1 4,821,554 - PS417_22245 0.38 -0.0 4,821,554 - PS417_22245 0.38 -0.9 4,821,823 + PS417_22245 0.66 +1.3 4,821,824 - PS417_22245 0.66 -0.4 4,821,922 + PS417_22245 0.76 +0.3 4,821,923 - PS417_22245 0.76 -0.8 4,821,997 + PS417_22245 0.84 +1.2 4,821,998 - PS417_22245 0.84 +0.8 4,821,998 - PS417_22245 0.84 -0.2 4,822,151 - -1.3 4,822,151 - -1.8 4,822,181 - +0.6 4,822,223 + +0.1 4,822,528 + PS417_22250 0.15 -0.2 4,822,636 + PS417_22250 0.24 -1.2 4,822,637 - PS417_22250 0.24 -0.5
Or see this region's nucleotide sequence