Strain Fitness in Pseudomonas simiae WCS417 around PS417_19725

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_19710 and PS417_19715 are separated by 398 nucleotidesPS417_19715 and PS417_19720 are separated by 18 nucleotidesPS417_19720 and PS417_19725 are separated by 135 nucleotidesPS417_19725 and PS417_19730 are separated by 3 nucleotidesPS417_19730 and PS417_19735 are separated by 4 nucleotides PS417_19710: PS417_19710 - flagellar biosynthesis protein FlhA, at 4,283,222 to 4,285,351 _19710 PS417_19715: PS417_19715 - cold-shock protein, at 4,285,750 to 4,285,953 _19715 PS417_19720: PS417_19720 - DNA-binding protein, at 4,285,972 to 4,286,184 _19720 PS417_19725: PS417_19725 - flagellar biosynthesis protein FlhB, at 4,286,320 to 4,287,456 _19725 PS417_19730: PS417_19730 - flagellar biosynthesis protein FliR, at 4,287,460 to 4,288,245 _19730 PS417_19735: PS417_19735 - flagellar biosynthesis protein FliQ, at 4,288,250 to 4,288,519 _19735 Position (kb) 4286 4287 4288Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 4285.483 kb on + strandat 4285.483 kb on + strandat 4285.483 kb on + strandat 4285.484 kb on - strandat 4285.511 kb on - strandat 4285.640 kb on - strandat 4285.693 kb on + strandat 4285.694 kb on - strandat 4285.694 kb on - strandat 4285.702 kb on + strandat 4285.703 kb on - strandat 4285.730 kb on + strandat 4285.731 kb on - strandat 4285.753 kb on + strandat 4285.927 kb on - strand, within PS417_19715at 4286.012 kb on + strand, within PS417_19720at 4286.153 kb on + strand, within PS417_19720at 4286.235 kb on - strandat 4286.237 kb on + strandat 4286.418 kb on - strandat 4286.418 kb on - strandat 4286.483 kb on + strand, within PS417_19725at 4286.484 kb on - strand, within PS417_19725at 4286.484 kb on - strand, within PS417_19725at 4286.725 kb on - strand, within PS417_19725at 4287.212 kb on + strand, within PS417_19725at 4287.212 kb on + strand, within PS417_19725at 4287.309 kb on - strand, within PS417_19725at 4287.485 kb on - strandat 4287.485 kb on - strandat 4287.485 kb on - strandat 4287.518 kb on + strandat 4288.104 kb on - strand, within PS417_19730at 4288.104 kb on - strand, within PS417_19730at 4288.124 kb on - strand, within PS417_19730at 4288.293 kb on + strand, within PS417_19735at 4288.293 kb on + strand, within PS417_19735at 4288.293 kb on + strand, within PS417_19735at 4288.294 kb on - strand, within PS417_19735

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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4,285,483 + +2.0
4,285,483 + +2.5
4,285,483 + +2.3
4,285,484 - -0.4
4,285,511 - -1.8
4,285,640 - -0.6
4,285,693 + +1.7
4,285,694 - +0.9
4,285,694 - -1.7
4,285,702 + +1.9
4,285,703 - +0.4
4,285,730 + +1.8
4,285,731 - -1.0
4,285,753 + +2.5
4,285,927 - PS417_19715 0.87 +2.4
4,286,012 + PS417_19720 0.19 +1.0
4,286,153 + PS417_19720 0.85 +1.6
4,286,235 - -0.8
4,286,237 + +1.5
4,286,418 - -5.0
4,286,418 - -0.8
4,286,483 + PS417_19725 0.14 -2.4
4,286,484 - PS417_19725 0.14 -4.5
4,286,484 - PS417_19725 0.14 -5.3
4,286,725 - PS417_19725 0.36 -2.8
4,287,212 + PS417_19725 0.78 -3.5
4,287,212 + PS417_19725 0.78 +0.4
4,287,309 - PS417_19725 0.87 -5.6
4,287,485 - -0.2
4,287,485 - +1.7
4,287,485 - +0.9
4,287,518 + -5.6
4,288,104 - PS417_19730 0.82 -4.0
4,288,104 - PS417_19730 0.82 -3.8
4,288,124 - PS417_19730 0.84 -3.8
4,288,293 + PS417_19735 0.16 -2.3
4,288,293 + PS417_19735 0.16 +0.3
4,288,293 + PS417_19735 0.16 -4.8
4,288,294 - PS417_19735 0.16 -4.3

Or see this region's nucleotide sequence