Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19710 and PS417_19715 are separated by 398 nucleotides PS417_19715 and PS417_19720 are separated by 18 nucleotides PS417_19720 and PS417_19725 are separated by 135 nucleotides PS417_19725 and PS417_19730 are separated by 3 nucleotides PS417_19730 and PS417_19735 are separated by 4 nucleotides
PS417_19710: PS417_19710 - flagellar biosynthesis protein FlhA, at 4,283,222 to 4,285,351
_19710
PS417_19715: PS417_19715 - cold-shock protein, at 4,285,750 to 4,285,953
_19715
PS417_19720: PS417_19720 - DNA-binding protein, at 4,285,972 to 4,286,184
_19720
PS417_19725: PS417_19725 - flagellar biosynthesis protein FlhB, at 4,286,320 to 4,287,456
_19725
PS417_19730: PS417_19730 - flagellar biosynthesis protein FliR, at 4,287,460 to 4,288,245
_19730
PS417_19735: PS417_19735 - flagellar biosynthesis protein FliQ, at 4,288,250 to 4,288,519
_19735
Position (kb)
4286
4287
4288 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4285.483 kb on + strand at 4285.483 kb on + strand at 4285.483 kb on + strand at 4285.484 kb on - strand at 4285.511 kb on - strand at 4285.640 kb on - strand at 4285.693 kb on + strand at 4285.694 kb on - strand at 4285.694 kb on - strand at 4285.702 kb on + strand at 4285.703 kb on - strand at 4285.730 kb on + strand at 4285.731 kb on - strand at 4285.753 kb on + strand at 4285.927 kb on - strand, within PS417_19715 at 4286.012 kb on + strand, within PS417_19720 at 4286.153 kb on + strand, within PS417_19720 at 4286.235 kb on - strand at 4286.237 kb on + strand at 4286.418 kb on - strand at 4286.418 kb on - strand at 4286.483 kb on + strand, within PS417_19725 at 4286.484 kb on - strand, within PS417_19725 at 4286.484 kb on - strand, within PS417_19725 at 4286.725 kb on - strand, within PS417_19725 at 4287.212 kb on + strand, within PS417_19725 at 4287.212 kb on + strand, within PS417_19725 at 4287.309 kb on - strand, within PS417_19725 at 4287.485 kb on - strand at 4287.485 kb on - strand at 4287.485 kb on - strand at 4287.518 kb on + strand at 4288.104 kb on - strand, within PS417_19730 at 4288.104 kb on - strand, within PS417_19730 at 4288.124 kb on - strand, within PS417_19730 at 4288.293 kb on + strand, within PS417_19735 at 4288.293 kb on + strand, within PS417_19735 at 4288.293 kb on + strand, within PS417_19735 at 4288.294 kb on - strand, within PS417_19735
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,285,483 + +2.0 4,285,483 + +2.5 4,285,483 + +2.3 4,285,484 - -0.4 4,285,511 - -1.8 4,285,640 - -0.6 4,285,693 + +1.7 4,285,694 - +0.9 4,285,694 - -1.7 4,285,702 + +1.9 4,285,703 - +0.4 4,285,730 + +1.8 4,285,731 - -1.0 4,285,753 + +2.5 4,285,927 - PS417_19715 0.87 +2.4 4,286,012 + PS417_19720 0.19 +1.0 4,286,153 + PS417_19720 0.85 +1.6 4,286,235 - -0.8 4,286,237 + +1.5 4,286,418 - -5.0 4,286,418 - -0.8 4,286,483 + PS417_19725 0.14 -2.4 4,286,484 - PS417_19725 0.14 -4.5 4,286,484 - PS417_19725 0.14 -5.3 4,286,725 - PS417_19725 0.36 -2.8 4,287,212 + PS417_19725 0.78 -3.5 4,287,212 + PS417_19725 0.78 +0.4 4,287,309 - PS417_19725 0.87 -5.6 4,287,485 - -0.2 4,287,485 - +1.7 4,287,485 - +0.9 4,287,518 + -5.6 4,288,104 - PS417_19730 0.82 -4.0 4,288,104 - PS417_19730 0.82 -3.8 4,288,124 - PS417_19730 0.84 -3.8 4,288,293 + PS417_19735 0.16 -2.3 4,288,293 + PS417_19735 0.16 +0.3 4,288,293 + PS417_19735 0.16 -4.8 4,288,294 - PS417_19735 0.16 -4.3
Or see this region's nucleotide sequence