Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07515 and PS417_07520 are separated by 191 nucleotides PS417_07520 and PS417_07525 are separated by 134 nucleotides PS417_07525 and PS417_07530 are separated by 13 nucleotides
PS417_07515: PS417_07515 - aconitate hydratase, at 1,651,324 to 1,654,065
_07515
PS417_07520: PS417_07520 - 23S rRNA methyltransferase, at 1,654,257 to 1,655,333
_07520
PS417_07525: PS417_07525 - sulfurtransferase, at 1,655,468 to 1,655,719
_07525
PS417_07530: PS417_07530 - multidrug transporter, at 1,655,733 to 1,657,127
_07530
Position (kb)
1654
1655
1656 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1653.461 kb on + strand, within PS417_07515 at 1653.506 kb on + strand, within PS417_07515 at 1653.596 kb on + strand, within PS417_07515 at 1653.597 kb on - strand, within PS417_07515 at 1653.597 kb on - strand, within PS417_07515 at 1653.597 kb on - strand, within PS417_07515 at 1653.785 kb on - strand, within PS417_07515 at 1654.001 kb on + strand at 1654.001 kb on + strand at 1654.001 kb on + strand at 1654.002 kb on - strand at 1654.002 kb on - strand at 1654.007 kb on + strand at 1654.007 kb on + strand at 1654.014 kb on + strand at 1654.015 kb on - strand at 1654.036 kb on - strand at 1654.036 kb on - strand at 1654.036 kb on - strand at 1654.119 kb on + strand at 1654.120 kb on - strand at 1654.128 kb on - strand at 1654.128 kb on - strand at 1654.128 kb on - strand at 1654.151 kb on - strand at 1654.164 kb on + strand at 1654.164 kb on + strand at 1654.165 kb on - strand at 1654.170 kb on + strand at 1654.170 kb on + strand at 1654.171 kb on - strand at 1654.271 kb on + strand at 1654.271 kb on + strand at 1655.028 kb on - strand, within PS417_07520 at 1655.262 kb on + strand at 1655.390 kb on + strand at 1655.390 kb on + strand at 1655.390 kb on + strand at 1655.390 kb on + strand at 1655.391 kb on - strand at 1655.404 kb on + strand at 1655.467 kb on + strand at 1655.467 kb on + strand at 1656.070 kb on + strand, within PS417_07530 at 1656.071 kb on - strand, within PS417_07530 at 1656.127 kb on + strand, within PS417_07530 at 1656.127 kb on + strand, within PS417_07530 at 1656.128 kb on - strand, within PS417_07530 at 1656.235 kb on + strand, within PS417_07530
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,653,461 + PS417_07515 0.78 -0.7 1,653,506 + PS417_07515 0.80 +0.6 1,653,596 + PS417_07515 0.83 -0.2 1,653,597 - PS417_07515 0.83 +0.6 1,653,597 - PS417_07515 0.83 -0.6 1,653,597 - PS417_07515 0.83 +0.1 1,653,785 - PS417_07515 0.90 +0.3 1,654,001 + +0.0 1,654,001 + +1.5 1,654,001 + -0.9 1,654,002 - -2.6 1,654,002 - -0.6 1,654,007 + -0.3 1,654,007 + -2.3 1,654,014 + -1.3 1,654,015 - +0.1 1,654,036 - +0.2 1,654,036 - -0.0 1,654,036 - -0.6 1,654,119 + +0.2 1,654,120 - +0.8 1,654,128 - +0.5 1,654,128 - +1.3 1,654,128 - +0.7 1,654,151 - +0.9 1,654,164 + -1.6 1,654,164 + -0.7 1,654,165 - +2.7 1,654,170 + -0.9 1,654,170 + -0.5 1,654,171 - +0.1 1,654,271 + +0.4 1,654,271 + -1.0 1,655,028 - PS417_07520 0.72 +0.8 1,655,262 + +0.7 1,655,390 + +2.2 1,655,390 + -0.2 1,655,390 + -1.4 1,655,390 + -1.0 1,655,391 - +1.7 1,655,404 + +0.7 1,655,467 + -1.1 1,655,467 + -0.4 1,656,070 + PS417_07530 0.24 -0.1 1,656,071 - PS417_07530 0.24 +0.0 1,656,127 + PS417_07530 0.28 +0.2 1,656,127 + PS417_07530 0.28 +0.1 1,656,128 - PS417_07530 0.28 -0.2 1,656,235 + PS417_07530 0.36 -0.2
Or see this region's nucleotide sequence