Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS15950

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS15945 and PFLU_RS15950 are separated by 13 nucleotidesPFLU_RS15950 and PFLU_RS15955 are separated by 14 nucleotidesPFLU_RS15955 and PFLU_RS31660 are separated by 92 nucleotides PFLU_RS15945: PFLU_RS15945 - MFS transporter, at 3,623,182 to 3,624,486 _RS15945 PFLU_RS15950: PFLU_RS15950 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 3,624,500 to 3,625,303 _RS15950 PFLU_RS15955: PFLU_RS15955 - 4-hydroxy-2-oxoheptanedioate aldolase, at 3,625,318 to 3,626,121 _RS15955 PFLU_RS31660: PFLU_RS31660 - helix-turn-helix domain-containing protein, at 3,626,214 to 3,626,309 _RS31660 Position (kb) 3624 3625 3626Strain fitness (log2 ratio) -3 -2 -1 0 1at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.951 kb on - strand, within PFLU_RS15945at 3623.951 kb on - strand, within PFLU_RS15945at 3623.961 kb on - strand, within PFLU_RS15945at 3624.359 kb on - strandat 3624.359 kb on - strandat 3624.410 kb on - strandat 3624.485 kb on - strandat 3624.491 kb on - strandat 3624.618 kb on - strand, within PFLU_RS15950at 3624.618 kb on - strand, within PFLU_RS15950at 3624.679 kb on + strand, within PFLU_RS15950at 3624.679 kb on + strand, within PFLU_RS15950at 3624.680 kb on - strand, within PFLU_RS15950at 3624.880 kb on - strand, within PFLU_RS15950at 3624.880 kb on - strand, within PFLU_RS15950at 3625.173 kb on - strand, within PFLU_RS15950at 3625.265 kb on + strandat 3625.265 kb on + strandat 3625.403 kb on - strand, within PFLU_RS15955at 3625.403 kb on - strand, within PFLU_RS15955at 3625.751 kb on + strand, within PFLU_RS15955at 3625.751 kb on + strand, within PFLU_RS15955at 3625.752 kb on - strand, within PFLU_RS15955at 3625.752 kb on - strand, within PFLU_RS15955at 3625.752 kb on - strand, within PFLU_RS15955at 3625.832 kb on + strand, within PFLU_RS15955at 3625.833 kb on - strand, within PFLU_RS15955at 3625.833 kb on - strand, within PFLU_RS15955at 3626.116 kb on + strandat 3626.116 kb on + strandat 3626.116 kb on + strandat 3626.117 kb on - strandat 3626.117 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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3,623,530 + PFLU_RS15945 0.27 +0.0
3,623,530 + PFLU_RS15945 0.27 -0.9
3,623,530 + PFLU_RS15945 0.27 -0.5
3,623,530 + PFLU_RS15945 0.27 +0.1
3,623,530 + PFLU_RS15945 0.27 +1.7
3,623,530 + PFLU_RS15945 0.27 -0.4
3,623,531 - PFLU_RS15945 0.27 -1.0
3,623,531 - PFLU_RS15945 0.27 +0.1
3,623,531 - PFLU_RS15945 0.27 +0.9
3,623,951 - PFLU_RS15945 0.59 +1.0
3,623,951 - PFLU_RS15945 0.59 -2.8
3,623,961 - PFLU_RS15945 0.60 -0.2
3,624,359 - -0.3
3,624,359 - -1.4
3,624,410 - -0.3
3,624,485 - +0.6
3,624,491 - -0.5
3,624,618 - PFLU_RS15950 0.15 +0.6
3,624,618 - PFLU_RS15950 0.15 -0.3
3,624,679 + PFLU_RS15950 0.22 +0.9
3,624,679 + PFLU_RS15950 0.22 +0.6
3,624,680 - PFLU_RS15950 0.22 -2.0
3,624,880 - PFLU_RS15950 0.47 +0.8
3,624,880 - PFLU_RS15950 0.47 -1.9
3,625,173 - PFLU_RS15950 0.84 +1.0
3,625,265 + +1.1
3,625,265 + -1.3
3,625,403 - PFLU_RS15955 0.11 +1.3
3,625,403 - PFLU_RS15955 0.11 -0.3
3,625,751 + PFLU_RS15955 0.54 +1.2
3,625,751 + PFLU_RS15955 0.54 +0.8
3,625,752 - PFLU_RS15955 0.54 -0.9
3,625,752 - PFLU_RS15955 0.54 +0.1
3,625,752 - PFLU_RS15955 0.54 -0.7
3,625,832 + PFLU_RS15955 0.64 -0.4
3,625,833 - PFLU_RS15955 0.64 -0.8
3,625,833 - PFLU_RS15955 0.64 +0.5
3,626,116 + +1.3
3,626,116 + -1.0
3,626,116 + -0.7
3,626,117 - -0.7
3,626,117 - +1.2

Or see this region's nucleotide sequence