Strain Fitness in Pseudomonas simiae WCS417 around PS417_22255

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_22250 and PS417_22255 are separated by 144 nucleotidesPS417_22255 and PS417_22260 are separated by 56 nucleotides PS417_22250: PS417_22250 - pseudouridine synthase, at 4,822,349 to 4,823,560 _22250 PS417_22255: PS417_22255 - Fe-S oxidoreductase, at 4,823,705 to 4,823,896 _22255 PS417_22260: PS417_22260 - segregation and condensation protein B, at 4,823,953 to 4,824,891 _22260 Position (kb) 4823 4824Strain fitness (log2 ratio) -1 0 1at 4823.631 kb on - strandat 4823.656 kb on - strandat 4823.897 kb on - strandat 4823.919 kb on - strandat 4823.919 kb on - strandat 4824.139 kb on + strand, within PS417_22260at 4824.140 kb on - strand, within PS417_22260at 4824.640 kb on + strand, within PS417_22260

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,823,631 - -0.5
4,823,656 - -0.7
4,823,897 - +1.1
4,823,919 - +0.2
4,823,919 - +0.3
4,824,139 + PS417_22260 0.20 -1.4
4,824,140 - PS417_22260 0.20 +1.1
4,824,640 + PS417_22260 0.73 -0.8

Or see this region's nucleotide sequence