Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19725 and PS417_19730 are separated by 3 nucleotides PS417_19730 and PS417_19735 are separated by 4 nucleotides PS417_19735 and PS417_19740 are separated by 4 nucleotides PS417_19740 and PS417_19745 overlap by 1 nucleotides
PS417_19725: PS417_19725 - flagellar biosynthesis protein FlhB, at 4,286,320 to 4,287,456
_19725
PS417_19730: PS417_19730 - flagellar biosynthesis protein FliR, at 4,287,460 to 4,288,245
_19730
PS417_19735: PS417_19735 - flagellar biosynthesis protein FliQ, at 4,288,250 to 4,288,519
_19735
PS417_19740: PS417_19740 - flagellar biosynthesis protein flip, at 4,288,524 to 4,289,267
_19740
PS417_19745: PS417_19745 - flagellar assembly protein FliO, at 4,289,267 to 4,289,710
_19745
Position (kb) 4288
4289 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4287.309 kb on - strand, within PS417_19725 at 4287.485 kb on - strand at 4287.485 kb on - strand at 4287.485 kb on - strand at 4287.518 kb on + strand at 4288.104 kb on - strand, within PS417_19730 at 4288.104 kb on - strand, within PS417_19730 at 4288.124 kb on - strand, within PS417_19730 at 4288.293 kb on + strand, within PS417_19735 at 4288.293 kb on + strand, within PS417_19735 at 4288.293 kb on + strand, within PS417_19735 at 4288.294 kb on - strand, within PS417_19735 at 4288.525 kb on + strand at 4288.853 kb on + strand, within PS417_19740 at 4288.854 kb on - strand, within PS417_19740 at 4288.854 kb on - strand, within PS417_19740 at 4289.106 kb on - strand, within PS417_19740 at 4289.268 kb on + strand at 4289.268 kb on + strand at 4289.383 kb on + strand, within PS417_19745
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,287,309 - PS417_19725 0.87 -5.4 4,287,485 - -0.0 4,287,485 - +0.9 4,287,485 - -0.2 4,287,518 + -5.5 4,288,104 - PS417_19730 0.82 -2.8 4,288,104 - PS417_19730 0.82 -1.6 4,288,124 - PS417_19730 0.84 -1.4 4,288,293 + PS417_19735 0.16 -2.1 4,288,293 + PS417_19735 0.16 -1.4 4,288,293 + PS417_19735 0.16 -4.0 4,288,294 - PS417_19735 0.16 -3.4 4,288,525 + -4.9 4,288,853 + PS417_19740 0.44 -3.6 4,288,854 - PS417_19740 0.44 +0.3 4,288,854 - PS417_19740 0.44 -5.5 4,289,106 - PS417_19740 0.78 -3.8 4,289,268 + -0.7 4,289,268 + -1.4 4,289,383 + PS417_19745 0.26 -3.0
Or see this region's nucleotide sequence