Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00085 and PS417_00090 overlap by 4 nucleotides PS417_00090 and PS417_00095 are separated by 69 nucleotides PS417_00095 and PS417_00100 are separated by 135 nucleotides
PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032
_00085
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
PS417_00095: PS417_00095 - peptide deformylase, at 21,052 to 21,558
_00095
PS417_00100: PS417_00100 - peptidoglycan-binding protein, at 21,694 to 22,470
_00100
Position (kb) 20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 19.777 kb on - strand, within PS417_00085 at 19.777 kb on - strand, within PS417_00085 at 20.997 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.565 kb on - strand at 21.585 kb on - strand at 21.675 kb on - strand at 21.678 kb on - strand at 21.678 kb on - strand at 21.678 kb on - strand at 21.720 kb on - strand at 21.720 kb on - strand at 21.856 kb on + strand, within PS417_00100 at 21.856 kb on + strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.857 kb on - strand, within PS417_00100 at 21.953 kb on + strand, within PS417_00100 at 21.973 kb on + strand, within PS417_00100
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 19,777 - PS417_00085 0.80 -1.1 19,777 - PS417_00085 0.80 -0.9 20,997 - -1.5 21,565 - -1.3 21,565 - -0.1 21,565 - +0.9 21,565 - +0.6 21,585 - +0.4 21,675 - +1.3 21,678 - -2.8 21,678 - -0.2 21,678 - +1.1 21,720 - -1.3 21,720 - -0.2 21,856 + PS417_00100 0.21 -1.7 21,856 + PS417_00100 0.21 -1.4 21,857 - PS417_00100 0.21 -0.4 21,857 - PS417_00100 0.21 -2.7 21,857 - PS417_00100 0.21 +0.4 21,953 + PS417_00100 0.33 -0.2 21,973 + PS417_00100 0.36 +0.2
Or see this region's nucleotide sequence