Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04275 and PS417_04280 are separated by 48 nucleotides PS417_04280 and PS417_04285 are separated by 16 nucleotides
PS417_04275: PS417_04275 - hypothetical protein, at 956,859 to 960,674
_04275
PS417_04280: PS417_04280 - carbon-nitrogen hydrolase, at 960,723 to 961,571
_04280
PS417_04285: PS417_04285 - protease TldD, at 961,588 to 963,030
_04285
Position (kb) 960
961
962 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 959.766 kb on + strand, within PS417_04275 at 959.767 kb on - strand, within PS417_04275 at 959.767 kb on - strand, within PS417_04275 at 959.767 kb on - strand, within PS417_04275 at 960.036 kb on + strand, within PS417_04275 at 960.036 kb on + strand, within PS417_04275 at 960.159 kb on + strand, within PS417_04275 at 960.159 kb on + strand, within PS417_04275 at 960.160 kb on - strand, within PS417_04275 at 960.160 kb on - strand, within PS417_04275 at 960.160 kb on - strand, within PS417_04275 at 960.334 kb on - strand at 960.334 kb on - strand at 960.497 kb on + strand at 960.498 kb on - strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.606 kb on + strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.607 kb on - strand at 960.687 kb on + strand at 960.687 kb on + strand at 960.687 kb on + strand at 960.687 kb on + strand at 960.688 kb on - strand at 960.833 kb on - strand, within PS417_04280 at 960.953 kb on - strand, within PS417_04280 at 960.953 kb on - strand, within PS417_04280 at 961.129 kb on - strand, within PS417_04280 at 961.195 kb on - strand, within PS417_04280 at 961.195 kb on - strand, within PS417_04280 at 961.339 kb on - strand, within PS417_04280 at 961.339 kb on - strand, within PS417_04280 at 961.390 kb on - strand, within PS417_04280 at 961.575 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.583 kb on + strand at 961.584 kb on - strand at 961.584 kb on - strand at 961.584 kb on - strand at 961.587 kb on + strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.588 kb on - strand at 961.709 kb on - strand at 961.709 kb on - strand at 961.831 kb on + strand, within PS417_04285 at 961.951 kb on + strand, within PS417_04285 at 961.952 kb on - strand, within PS417_04285 at 962.228 kb on - strand, within PS417_04285 at 962.422 kb on + strand, within PS417_04285
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 959,766 + PS417_04275 0.76 -1.0 959,767 - PS417_04275 0.76 -1.1 959,767 - PS417_04275 0.76 +1.2 959,767 - PS417_04275 0.76 -0.0 960,036 + PS417_04275 0.83 -1.3 960,036 + PS417_04275 0.83 +0.1 960,159 + PS417_04275 0.86 +0.7 960,159 + PS417_04275 0.86 +1.7 960,160 - PS417_04275 0.87 -1.0 960,160 - PS417_04275 0.87 -2.7 960,160 - PS417_04275 0.87 +0.2 960,334 - -1.1 960,334 - -1.3 960,497 + -0.1 960,498 - -0.6 960,606 + -0.6 960,606 + +0.5 960,606 + +0.2 960,606 + -0.3 960,606 + +1.2 960,606 + +0.0 960,607 - +0.8 960,607 - -0.6 960,607 - -0.2 960,607 - -1.5 960,607 - -0.8 960,687 + +1.1 960,687 + +2.4 960,687 + +0.5 960,687 + +1.2 960,688 - -0.6 960,833 - PS417_04280 0.13 -0.9 960,953 - PS417_04280 0.27 -2.0 960,953 - PS417_04280 0.27 -0.9 961,129 - PS417_04280 0.48 +0.4 961,195 - PS417_04280 0.56 -2.6 961,195 - PS417_04280 0.56 -1.3 961,339 - PS417_04280 0.73 -0.8 961,339 - PS417_04280 0.73 -1.7 961,390 - PS417_04280 0.79 -0.6 961,575 + -0.8 961,583 + +0.6 961,583 + -0.8 961,583 + -0.4 961,583 + +1.2 961,583 + -1.2 961,584 - -0.4 961,584 - +0.2 961,584 - -0.4 961,587 + -0.2 961,588 - -0.9 961,588 - +0.1 961,588 - -0.8 961,588 - -2.9 961,588 - +0.6 961,588 - -1.7 961,709 - -0.7 961,709 - -0.4 961,831 + PS417_04285 0.17 -1.6 961,951 + PS417_04285 0.25 -0.6 961,952 - PS417_04285 0.25 -1.0 962,228 - PS417_04285 0.44 -0.8 962,422 + PS417_04285 0.58 +0.1
Or see this region's nucleotide sequence