Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00390 and PS417_00395 are separated by 102 nucleotides PS417_00395 and PS417_00400 are separated by 56 nucleotides PS417_00400 and PS417_00405 are separated by 74 nucleotides
PS417_00390: PS417_00390 - ABC transporter substrate-binding protein, at 75,580 to 76,500
_00390
PS417_00395: PS417_00395 - serine kinase, at 76,603 to 77,556
_00395
PS417_00400: PS417_00400 - hypothetical protein, at 77,613 to 77,903
_00400
PS417_00405: PS417_00405 - DNA polymerase I, at 77,978 to 80,755
_00405
Position (kb) 76
77
78 Strain fitness (log2 ratio) -2
-1
0
1 at 75.660 kb on - strand at 75.756 kb on + strand, within PS417_00390 at 75.756 kb on + strand, within PS417_00390 at 75.757 kb on - strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.761 kb on - strand, within PS417_00390 at 76.099 kb on - strand, within PS417_00390 at 76.174 kb on + strand, within PS417_00390 at 76.259 kb on + strand, within PS417_00390 at 76.532 kb on + strand at 76.532 kb on + strand at 76.605 kb on - strand at 76.808 kb on + strand, within PS417_00395 at 77.186 kb on - strand, within PS417_00395 at 77.607 kb on - strand at 77.652 kb on - strand, within PS417_00400 at 77.897 kb on + strand at 77.897 kb on + strand at 77.897 kb on + strand at 77.898 kb on - strand at 77.898 kb on - strand at 77.898 kb on - strand at 77.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 75,660 - +0.3 75,756 + PS417_00390 0.19 -0.5 75,756 + PS417_00390 0.19 -0.9 75,757 - PS417_00390 0.19 -1.3 75,760 + PS417_00390 0.20 -1.0 75,760 + PS417_00390 0.20 +1.4 75,761 - PS417_00390 0.20 -0.4 76,099 - PS417_00390 0.56 -0.5 76,174 + PS417_00390 0.64 -0.9 76,259 + PS417_00390 0.74 -1.2 76,532 + +1.1 76,532 + -0.4 76,605 - -1.7 76,808 + PS417_00395 0.21 -1.4 77,186 - PS417_00395 0.61 -2.6 77,607 - -0.9 77,652 - PS417_00400 0.13 -1.5 77,897 + +0.4 77,897 + +0.2 77,897 + -2.4 77,898 - -0.4 77,898 - +0.3 77,898 - -1.6 77,906 + -0.5
Or see this region's nucleotide sequence