Strain Fitness in Pseudomonas simiae WCS417 around PS417_19740

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_19730 and PS417_19735 are separated by 4 nucleotidesPS417_19735 and PS417_19740 are separated by 4 nucleotidesPS417_19740 and PS417_19745 overlap by 1 nucleotidesPS417_19745 and PS417_19750 are separated by 1 nucleotidesPS417_19750 and PS417_19755 are separated by 46 nucleotides PS417_19730: PS417_19730 - flagellar biosynthesis protein FliR, at 4,287,460 to 4,288,245 _19730 PS417_19735: PS417_19735 - flagellar biosynthesis protein FliQ, at 4,288,250 to 4,288,519 _19735 PS417_19740: PS417_19740 - flagellar biosynthesis protein flip, at 4,288,524 to 4,289,267 _19740 PS417_19745: PS417_19745 - flagellar assembly protein FliO, at 4,289,267 to 4,289,710 _19745 PS417_19750: PS417_19750 - flagellar motor switch protein, at 4,289,712 to 4,290,179 _19750 PS417_19755: PS417_19755 - flagellar motor switch protein FliM, at 4,290,226 to 4,291,194 _19755 Position (kb) 4288 4289 4290Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4288.104 kb on - strand, within PS417_19730at 4288.104 kb on - strand, within PS417_19730at 4288.124 kb on - strand, within PS417_19730at 4288.293 kb on + strand, within PS417_19735at 4288.293 kb on + strand, within PS417_19735at 4288.293 kb on + strand, within PS417_19735at 4288.294 kb on - strand, within PS417_19735at 4288.525 kb on + strandat 4288.853 kb on + strand, within PS417_19740at 4288.854 kb on - strand, within PS417_19740at 4288.854 kb on - strand, within PS417_19740at 4289.106 kb on - strand, within PS417_19740at 4289.268 kb on + strandat 4289.268 kb on + strandat 4289.383 kb on + strand, within PS417_19745at 4289.612 kb on + strand, within PS417_19745at 4289.613 kb on - strand, within PS417_19745at 4289.613 kb on - strand, within PS417_19745at 4289.704 kb on - strandat 4289.704 kb on - strandat 4290.016 kb on + strand, within PS417_19750at 4290.180 kb on + strandat 4290.180 kb on + strandat 4290.180 kb on + strandat 4290.181 kb on - strandat 4290.181 kb on - strandat 4290.181 kb on - strandat 4290.181 kb on - strandat 4290.213 kb on + strandat 4290.213 kb on + strandat 4290.214 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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4,288,104 - PS417_19730 0.82 -2.2
4,288,104 - PS417_19730 0.82 -3.6
4,288,124 - PS417_19730 0.84 -3.7
4,288,293 + PS417_19735 0.16 -2.1
4,288,293 + PS417_19735 0.16 -3.0
4,288,293 + PS417_19735 0.16 -3.1
4,288,294 - PS417_19735 0.16 -2.8
4,288,525 + -3.3
4,288,853 + PS417_19740 0.44 -1.6
4,288,854 - PS417_19740 0.44 -1.7
4,288,854 - PS417_19740 0.44 -5.8
4,289,106 - PS417_19740 0.78 -3.9
4,289,268 + -0.7
4,289,268 + -1.4
4,289,383 + PS417_19745 0.26 -4.0
4,289,612 + PS417_19745 0.78 -5.0
4,289,613 - PS417_19745 0.78 -5.7
4,289,613 - PS417_19745 0.78 -2.6
4,289,704 - -1.8
4,289,704 - -3.2
4,290,016 + PS417_19750 0.65 -3.0
4,290,180 + -3.3
4,290,180 + -0.8
4,290,180 + -2.1
4,290,181 - -2.3
4,290,181 - -6.4
4,290,181 - -1.9
4,290,181 - -3.2
4,290,213 + -2.7
4,290,213 + -1.9
4,290,214 - +0.4

Or see this region's nucleotide sequence