Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19105 and PS417_19110 are separated by 82 nucleotides PS417_19110 and PS417_19115 are separated by 90 nucleotides PS417_19115 and PS417_19120 overlap by 9 nucleotides PS417_19120 and PS417_19125 are separated by 60 nucleotides PS417_19125 and PS417_19130 are separated by 101 nucleotides
PS417_19105: PS417_19105 - hypothetical protein, at 4,129,476 to 4,129,697
_19105
PS417_19110: PS417_19110 - hypothetical protein, at 4,129,780 to 4,130,079
_19110
PS417_19115: PS417_19115 - hypothetical protein, at 4,130,170 to 4,130,520
_19115
PS417_19120: PS417_19120 - tRNA-Asn, at 4,130,512 to 4,130,587
_19120
PS417_19125: PS417_19125 - LysR family transcriptional regulator, at 4,130,648 to 4,131,544
_19125
PS417_19130: PS417_19130 - L-lactate dehydrogenase (cytochrome) (EC 1.1.2.3) (from data) , at 4,131,646 to 4,132,776
_19130
Position (kb)
4130
4131
4132 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4129.672 kb on + strand at 4129.672 kb on + strand, within PS417_19105 at 4129.672 kb on + strand, within PS417_19105 at 4129.673 kb on - strand, within PS417_19105 at 4129.673 kb on - strand, within PS417_19105 at 4129.695 kb on + strand at 4129.695 kb on + strand at 4129.832 kb on - strand, within PS417_19110 at 4129.832 kb on - strand, within PS417_19110 at 4129.848 kb on + strand, within PS417_19110 at 4129.848 kb on + strand, within PS417_19110 at 4129.849 kb on - strand, within PS417_19110 at 4129.852 kb on + strand, within PS417_19110 at 4129.852 kb on + strand, within PS417_19110 at 4129.925 kb on + strand, within PS417_19110 at 4129.926 kb on - strand, within PS417_19110 at 4129.926 kb on - strand, within PS417_19110 at 4129.959 kb on + strand, within PS417_19110 at 4129.959 kb on + strand, within PS417_19110 at 4130.001 kb on + strand, within PS417_19110 at 4130.001 kb on + strand, within PS417_19110 at 4130.014 kb on + strand, within PS417_19110 at 4130.157 kb on + strand at 4130.158 kb on - strand at 4130.158 kb on - strand at 4130.158 kb on - strand at 4130.158 kb on - strand at 4130.161 kb on + strand at 4130.161 kb on + strand at 4130.162 kb on - strand at 4130.162 kb on - strand at 4130.206 kb on - strand, within PS417_19115 at 4130.206 kb on - strand, within PS417_19115 at 4130.206 kb on - strand, within PS417_19115 at 4130.211 kb on + strand, within PS417_19115 at 4130.211 kb on + strand, within PS417_19115 at 4130.211 kb on + strand, within PS417_19115 at 4130.211 kb on + strand, within PS417_19115 at 4130.211 kb on + strand, within PS417_19115 at 4130.211 kb on + strand, within PS417_19115 at 4130.212 kb on - strand, within PS417_19115 at 4130.212 kb on - strand at 4130.212 kb on - strand, within PS417_19115 at 4130.212 kb on - strand, within PS417_19115 at 4130.212 kb on - strand, within PS417_19115 at 4130.212 kb on - strand, within PS417_19115 at 4130.213 kb on + strand, within PS417_19115 at 4130.214 kb on - strand, within PS417_19115 at 4130.214 kb on - strand, within PS417_19115 at 4130.214 kb on - strand, within PS417_19115 at 4130.342 kb on - strand, within PS417_19115 at 4130.551 kb on + strand, within PS417_19120 at 4130.811 kb on + strand, within PS417_19125 at 4131.013 kb on + strand, within PS417_19125 at 4131.014 kb on - strand, within PS417_19125 at 4131.207 kb on + strand, within PS417_19125 at 4131.207 kb on + strand, within PS417_19125 at 4131.644 kb on + strand at 4131.644 kb on + strand at 4131.652 kb on - strand at 4131.652 kb on - strand at 4131.652 kb on - strand at 4131.895 kb on + strand, within PS417_19130 at 4131.895 kb on + strand, within PS417_19130 at 4131.895 kb on + strand, within PS417_19130 at 4131.895 kb on + strand, within PS417_19130 at 4131.896 kb on - strand, within PS417_19130 at 4131.896 kb on - strand, within PS417_19130 at 4131.896 kb on - strand, within PS417_19130 at 4131.896 kb on - strand, within PS417_19130 at 4131.896 kb on - strand, within PS417_19130 at 4131.896 kb on - strand, within PS417_19130 at 4132.030 kb on + strand, within PS417_19130 at 4132.030 kb on + strand, within PS417_19130 at 4132.030 kb on + strand, within PS417_19130 at 4132.030 kb on + strand, within PS417_19130 at 4132.031 kb on - strand, within PS417_19130 at 4132.031 kb on - strand, within PS417_19130 at 4132.031 kb on - strand, within PS417_19130 at 4132.104 kb on - strand, within PS417_19130 at 4132.104 kb on - strand, within PS417_19130 at 4132.107 kb on + strand, within PS417_19130 at 4132.263 kb on + strand, within PS417_19130 at 4132.264 kb on - strand, within PS417_19130 at 4132.303 kb on + strand, within PS417_19130 at 4132.304 kb on - strand, within PS417_19130 at 4132.304 kb on - strand, within PS417_19130 at 4132.402 kb on - strand, within PS417_19130
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 4,129,672 + -1.1 4,129,672 + PS417_19105 0.88 +0.4 4,129,672 + PS417_19105 0.88 -1.1 4,129,673 - PS417_19105 0.89 +0.5 4,129,673 - PS417_19105 0.89 +0.4 4,129,695 + +0.7 4,129,695 + +0.7 4,129,832 - PS417_19110 0.17 -0.4 4,129,832 - PS417_19110 0.17 -1.4 4,129,848 + PS417_19110 0.23 -1.4 4,129,848 + PS417_19110 0.23 -0.4 4,129,849 - PS417_19110 0.23 +0.7 4,129,852 + PS417_19110 0.24 +0.0 4,129,852 + PS417_19110 0.24 +0.8 4,129,925 + PS417_19110 0.48 -1.0 4,129,926 - PS417_19110 0.49 -0.8 4,129,926 - PS417_19110 0.49 +0.6 4,129,959 + PS417_19110 0.60 +1.3 4,129,959 + PS417_19110 0.60 -0.4 4,130,001 + PS417_19110 0.74 +0.1 4,130,001 + PS417_19110 0.74 -1.6 4,130,014 + PS417_19110 0.78 +0.3 4,130,157 + -0.0 4,130,158 - -0.7 4,130,158 - +0.3 4,130,158 - -0.5 4,130,158 - -0.7 4,130,161 + +0.2 4,130,161 + +0.4 4,130,162 - -0.7 4,130,162 - +0.8 4,130,206 - PS417_19115 0.10 +0.9 4,130,206 - PS417_19115 0.10 +2.3 4,130,206 - PS417_19115 0.10 -0.8 4,130,211 + PS417_19115 0.12 -0.7 4,130,211 + PS417_19115 0.12 +0.7 4,130,211 + PS417_19115 0.12 -0.4 4,130,211 + PS417_19115 0.12 +0.2 4,130,211 + PS417_19115 0.12 +0.1 4,130,211 + PS417_19115 0.12 +1.6 4,130,212 - PS417_19115 0.12 -1.1 4,130,212 - +3.0 4,130,212 - PS417_19115 0.12 -0.5 4,130,212 - PS417_19115 0.12 -0.1 4,130,212 - PS417_19115 0.12 +1.5 4,130,212 - PS417_19115 0.12 -1.4 4,130,213 + PS417_19115 0.12 -0.7 4,130,214 - PS417_19115 0.13 +0.2 4,130,214 - PS417_19115 0.13 +0.2 4,130,214 - PS417_19115 0.13 -1.3 4,130,342 - PS417_19115 0.49 -1.2 4,130,551 + PS417_19120 0.51 +0.3 4,130,811 + PS417_19125 0.18 -1.9 4,131,013 + PS417_19125 0.41 -2.1 4,131,014 - PS417_19125 0.41 +0.7 4,131,207 + PS417_19125 0.62 +1.3 4,131,207 + PS417_19125 0.62 +2.1 4,131,644 + +0.5 4,131,644 + -1.0 4,131,652 - +1.3 4,131,652 - -0.4 4,131,652 - -3.3 4,131,895 + PS417_19130 0.22 -0.3 4,131,895 + PS417_19130 0.22 -1.4 4,131,895 + PS417_19130 0.22 -0.7 4,131,895 + PS417_19130 0.22 -0.3 4,131,896 - PS417_19130 0.22 +0.3 4,131,896 - PS417_19130 0.22 -2.1 4,131,896 - PS417_19130 0.22 +0.1 4,131,896 - PS417_19130 0.22 -0.0 4,131,896 - PS417_19130 0.22 +0.2 4,131,896 - PS417_19130 0.22 -0.1 4,132,030 + PS417_19130 0.34 +0.1 4,132,030 + PS417_19130 0.34 -0.1 4,132,030 + PS417_19130 0.34 -0.7 4,132,030 + PS417_19130 0.34 +1.8 4,132,031 - PS417_19130 0.34 -0.7 4,132,031 - PS417_19130 0.34 -0.4 4,132,031 - PS417_19130 0.34 +0.2 4,132,104 - PS417_19130 0.40 +0.3 4,132,104 - PS417_19130 0.40 -0.6 4,132,107 + PS417_19130 0.41 +0.1 4,132,263 + PS417_19130 0.55 -0.5 4,132,264 - PS417_19130 0.55 +0.6 4,132,303 + PS417_19130 0.58 -0.1 4,132,304 - PS417_19130 0.58 -1.4 4,132,304 - PS417_19130 0.58 -0.1 4,132,402 - PS417_19130 0.67 -1.7
Or see this region's nucleotide sequence