Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14400 and PS417_14405 are separated by 111 nucleotides PS417_14405 and PS417_14410 overlap by 4 nucleotides PS417_14410 and PS417_14415 overlap by 14 nucleotides PS417_14415 and PS417_14420 overlap by 4 nucleotides
PS417_14400: PS417_14400 - hypothetical protein, at 3,148,935 to 3,149,123
_14400
PS417_14405: PS417_14405 - ABC transporter permease, at 3,149,235 to 3,149,996
_14405
PS417_14410: PS417_14410 - ABC transporter substrate-binding protein, at 3,149,993 to 3,151,192
_14410
PS417_14415: PS417_14415 - ABC transporter ATP-binding protein, at 3,151,179 to 3,151,982
_14415
PS417_14420: PS417_14420 - acyl-CoA dehydrogenase, at 3,151,979 to 3,153,040
_14420
Position (kb)
3149
3150
3151
3152 Strain fitness (log2 ratio)
-1
0
1 at 3149.047 kb on + strand, within PS417_14400 at 3149.074 kb on - strand, within PS417_14400 at 3149.365 kb on + strand, within PS417_14405 at 3149.366 kb on - strand, within PS417_14405 at 3149.366 kb on - strand, within PS417_14405 at 3149.368 kb on + strand, within PS417_14405 at 3149.443 kb on - strand, within PS417_14405 at 3149.811 kb on + strand, within PS417_14405 at 3149.811 kb on + strand, within PS417_14405 at 3149.812 kb on - strand, within PS417_14405 at 3149.821 kb on + strand, within PS417_14405 at 3150.177 kb on - strand, within PS417_14410 at 3150.271 kb on - strand, within PS417_14410 at 3150.271 kb on - strand, within PS417_14410 at 3150.271 kb on - strand, within PS417_14410 at 3150.271 kb on - strand, within PS417_14410 at 3151.253 kb on + strand at 3151.253 kb on + strand at 3151.359 kb on + strand, within PS417_14415 at 3151.982 kb on + strand at 3151.982 kb on + strand at 3151.982 kb on + strand at 3151.983 kb on - strand at 3151.983 kb on - strand at 3151.983 kb on - strand at 3152.097 kb on + strand, within PS417_14420
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,149,047 + PS417_14400 0.59 +0.6 3,149,074 - PS417_14400 0.74 +0.1 3,149,365 + PS417_14405 0.17 +1.0 3,149,366 - PS417_14405 0.17 -0.2 3,149,366 - PS417_14405 0.17 +0.1 3,149,368 + PS417_14405 0.17 +0.1 3,149,443 - PS417_14405 0.27 -0.6 3,149,811 + PS417_14405 0.76 +0.3 3,149,811 + PS417_14405 0.76 -0.0 3,149,812 - PS417_14405 0.76 +0.1 3,149,821 + PS417_14405 0.77 -0.3 3,150,177 - PS417_14410 0.15 -0.1 3,150,271 - PS417_14410 0.23 +0.8 3,150,271 - PS417_14410 0.23 +1.0 3,150,271 - PS417_14410 0.23 +0.7 3,150,271 - PS417_14410 0.23 -0.2 3,151,253 + -0.3 3,151,253 + +0.2 3,151,359 + PS417_14415 0.22 +0.7 3,151,982 + +1.0 3,151,982 + -0.0 3,151,982 + -0.3 3,151,983 - +1.1 3,151,983 - +0.7 3,151,983 - +0.1 3,152,097 + PS417_14420 0.11 +0.0
Or see this region's nucleotide sequence