Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12465 and PS417_12470 are separated by 108 nucleotides PS417_12470 and PS417_12475 are separated by 50 nucleotides PS417_12475 and PS417_12480 are separated by 40 nucleotides
PS417_12465: PS417_12465 - Fis family transcriptional regulator, at 2,710,807 to 2,712,132
_12465
PS417_12470: PS417_12470 - hypothetical protein, at 2,712,241 to 2,713,542
_12470
PS417_12475: PS417_12475 - hypothetical protein, at 2,713,593 to 2,714,336
_12475
PS417_12480: PS417_12480 - peptidase C39, at 2,714,377 to 2,715,057
_12480
Position (kb)
2712
2713
2714 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2711.544 kb on - strand, within PS417_12465 at 2711.544 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.786 kb on - strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.983 kb on - strand, within PS417_12465 at 2712.249 kb on - strand at 2712.249 kb on - strand at 2712.365 kb on + strand at 2712.465 kb on + strand at 2712.493 kb on + strand, within PS417_12470 at 2712.591 kb on + strand, within PS417_12470 at 2712.599 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.859 kb on - strand, within PS417_12470 at 2712.923 kb on + strand, within PS417_12470 at 2712.987 kb on - strand, within PS417_12470 at 2713.041 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.057 kb on - strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470 at 2713.173 kb on - strand, within PS417_12470 at 2713.475 kb on + strand at 2713.475 kb on + strand at 2713.475 kb on + strand at 2713.500 kb on + strand at 2713.501 kb on - strand at 2713.501 kb on - strand at 2713.501 kb on - strand at 2713.523 kb on - strand at 2713.547 kb on + strand at 2713.589 kb on + strand at 2713.590 kb on - strand at 2713.743 kb on - strand, within PS417_12475 at 2713.778 kb on + strand, within PS417_12475 at 2713.938 kb on + strand, within PS417_12475 at 2713.938 kb on + strand, within PS417_12475 at 2713.938 kb on + strand, within PS417_12475 at 2713.939 kb on - strand, within PS417_12475 at 2713.939 kb on - strand at 2713.939 kb on - strand, within PS417_12475 at 2713.948 kb on + strand, within PS417_12475 at 2713.955 kb on + strand, within PS417_12475 at 2713.955 kb on + strand, within PS417_12475 at 2713.973 kb on + strand, within PS417_12475 at 2713.973 kb on + strand, within PS417_12475 at 2714.082 kb on + strand, within PS417_12475 at 2714.082 kb on + strand, within PS417_12475 at 2714.083 kb on - strand, within PS417_12475 at 2714.086 kb on + strand, within PS417_12475 at 2714.087 kb on - strand, within PS417_12475 at 2714.144 kb on + strand, within PS417_12475 at 2714.145 kb on - strand, within PS417_12475 at 2714.196 kb on - strand, within PS417_12475 at 2714.216 kb on - strand, within PS417_12475 at 2714.282 kb on - strand at 2714.282 kb on - strand at 2714.364 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,711,544 - PS417_12465 0.56 +0.7 2,711,544 - PS417_12465 0.56 +0.6 2,711,693 - PS417_12465 0.67 -0.9 2,711,693 - PS417_12465 0.67 +1.3 2,711,753 + PS417_12465 0.71 -1.9 2,711,753 + PS417_12465 0.71 -0.1 2,711,753 + PS417_12465 0.71 +1.1 2,711,786 - PS417_12465 0.74 -0.6 2,711,927 + PS417_12465 0.84 +0.7 2,711,927 + PS417_12465 0.84 +0.0 2,711,927 + PS417_12465 0.84 -0.8 2,711,927 + PS417_12465 0.84 +0.6 2,711,928 - PS417_12465 0.85 -0.5 2,711,928 - PS417_12465 0.85 +0.2 2,711,928 - PS417_12465 0.85 +0.4 2,711,983 - PS417_12465 0.89 +2.3 2,712,249 - -0.9 2,712,249 - -0.4 2,712,365 + -0.9 2,712,465 + +0.7 2,712,493 + PS417_12470 0.19 +0.5 2,712,591 + PS417_12470 0.27 -0.9 2,712,599 + PS417_12470 0.27 +0.4 2,712,704 + PS417_12470 0.36 -0.7 2,712,704 + PS417_12470 0.36 -1.3 2,712,705 - PS417_12470 0.36 -1.6 2,712,705 - PS417_12470 0.36 -1.7 2,712,705 - PS417_12470 0.36 -1.8 2,712,859 - PS417_12470 0.47 -0.1 2,712,923 + PS417_12470 0.52 -0.8 2,712,987 - PS417_12470 0.57 -0.6 2,713,041 + PS417_12470 0.61 -0.6 2,713,056 + PS417_12470 0.63 -1.2 2,713,056 + PS417_12470 0.63 -0.0 2,713,057 - PS417_12470 0.63 +0.1 2,713,112 + PS417_12470 0.67 +0.1 2,713,112 + PS417_12470 0.67 +0.1 2,713,173 - PS417_12470 0.72 +0.2 2,713,475 + -0.8 2,713,475 + +0.3 2,713,475 + -0.6 2,713,500 + -0.3 2,713,501 - -2.5 2,713,501 - +0.3 2,713,501 - -0.2 2,713,523 - +0.0 2,713,547 + +2.0 2,713,589 + -0.5 2,713,590 - +0.6 2,713,743 - PS417_12475 0.20 -0.2 2,713,778 + PS417_12475 0.25 -0.2 2,713,938 + PS417_12475 0.46 -0.0 2,713,938 + PS417_12475 0.46 -1.3 2,713,938 + PS417_12475 0.46 -1.2 2,713,939 - PS417_12475 0.47 +0.6 2,713,939 - +0.7 2,713,939 - PS417_12475 0.47 -0.3 2,713,948 + PS417_12475 0.48 +0.2 2,713,955 + PS417_12475 0.49 -0.2 2,713,955 + PS417_12475 0.49 -0.5 2,713,973 + PS417_12475 0.51 -0.8 2,713,973 + PS417_12475 0.51 -3.4 2,714,082 + PS417_12475 0.66 -0.0 2,714,082 + PS417_12475 0.66 +1.8 2,714,083 - PS417_12475 0.66 -0.9 2,714,086 + PS417_12475 0.66 +0.3 2,714,087 - PS417_12475 0.66 -0.5 2,714,144 + PS417_12475 0.74 -1.2 2,714,145 - PS417_12475 0.74 +0.2 2,714,196 - PS417_12475 0.81 -0.2 2,714,216 - PS417_12475 0.84 -2.6 2,714,282 - -0.9 2,714,282 - +0.1 2,714,364 + -1.5
Or see this region's nucleotide sequence