Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11220 and PS417_11225 overlap by 4 nucleotides PS417_11225 and PS417_11230 are separated by 147 nucleotides
PS417_11220: PS417_11220 - signal peptide protein, at 2,405,546 to 2,406,937
_11220
PS417_11225: PS417_11225 - glyoxalase, at 2,406,934 to 2,407,830
_11225
PS417_11230: PS417_11230 - hydroxyacid dehydrogenase, at 2,407,978 to 2,409,045
_11230
Position (kb)
2406
2407
2408 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2406.013 kb on + strand at 2406.016 kb on - strand, within PS417_11220 at 2406.171 kb on + strand, within PS417_11220 at 2406.192 kb on + strand, within PS417_11220 at 2406.201 kb on + strand, within PS417_11220 at 2406.201 kb on + strand, within PS417_11220 at 2406.559 kb on - strand, within PS417_11220 at 2406.646 kb on - strand, within PS417_11220 at 2406.766 kb on - strand, within PS417_11220 at 2406.766 kb on - strand, within PS417_11220 at 2407.279 kb on - strand, within PS417_11225 at 2407.624 kb on + strand, within PS417_11225 at 2407.624 kb on + strand, within PS417_11225 at 2407.624 kb on + strand, within PS417_11225 at 2407.625 kb on - strand, within PS417_11225 at 2407.625 kb on - strand, within PS417_11225 at 2407.706 kb on + strand, within PS417_11225 at 2407.724 kb on + strand, within PS417_11225 at 2407.932 kb on - strand at 2407.936 kb on + strand at 2407.963 kb on - strand at 2407.976 kb on + strand at 2407.976 kb on + strand at 2407.976 kb on + strand at 2407.976 kb on + strand at 2407.991 kb on - strand at 2407.996 kb on + strand at 2408.069 kb on + strand at 2408.324 kb on - strand, within PS417_11230 at 2408.324 kb on - strand, within PS417_11230 at 2408.470 kb on + strand at 2408.471 kb on - strand, within PS417_11230
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,406,013 + +0.7 2,406,016 - PS417_11220 0.34 +0.6 2,406,171 + PS417_11220 0.45 -0.8 2,406,192 + PS417_11220 0.46 +0.4 2,406,201 + PS417_11220 0.47 -0.2 2,406,201 + PS417_11220 0.47 -0.6 2,406,559 - PS417_11220 0.73 -0.5 2,406,646 - PS417_11220 0.79 -0.4 2,406,766 - PS417_11220 0.88 -0.1 2,406,766 - PS417_11220 0.88 +0.7 2,407,279 - PS417_11225 0.38 -3.1 2,407,624 + PS417_11225 0.77 -0.7 2,407,624 + PS417_11225 0.77 -2.0 2,407,624 + PS417_11225 0.77 -0.3 2,407,625 - PS417_11225 0.77 -0.3 2,407,625 - PS417_11225 0.77 -0.6 2,407,706 + PS417_11225 0.86 -0.5 2,407,724 + PS417_11225 0.88 -0.1 2,407,932 - +0.4 2,407,936 + -0.8 2,407,963 - +0.1 2,407,976 + +0.1 2,407,976 + -0.2 2,407,976 + -2.4 2,407,976 + +0.7 2,407,991 - -0.5 2,407,996 + -0.8 2,408,069 + +0.6 2,408,324 - PS417_11230 0.32 +1.8 2,408,324 - PS417_11230 0.32 -0.4 2,408,470 + +1.7 2,408,471 - PS417_11230 0.46 +0.8
Or see this region's nucleotide sequence