Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08840 and PS417_08845 are separated by 235 nucleotides PS417_08845 and PS417_08850 are separated by 65 nucleotides PS417_08850 and PS417_08855 are separated by 332 nucleotides
PS417_08840: PS417_08840 - serine/threonine protein kinase, at 1,922,331 to 1,923,629
_08840
PS417_08845: PS417_08845 - hypothetical protein, at 1,923,865 to 1,924,233
_08845
PS417_08850: PS417_08850 - beta (1-6) glucans synthase, at 1,924,299 to 1,925,852
_08850
PS417_08855: PS417_08855 - glycine/betaine ABC transporter substrate-binding protein, at 1,926,185 to 1,927,036
_08855
Position (kb)
1924
1925
1926 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1923.440 kb on - strand, within PS417_08840 at 1923.541 kb on + strand at 1923.541 kb on + strand at 1923.541 kb on + strand at 1923.634 kb on + strand at 1923.635 kb on - strand at 1923.653 kb on + strand at 1923.653 kb on + strand at 1923.654 kb on - strand at 1923.657 kb on + strand at 1923.658 kb on - strand at 1923.658 kb on - strand at 1923.658 kb on - strand at 1923.674 kb on + strand at 1923.674 kb on + strand at 1923.674 kb on + strand at 1923.747 kb on + strand at 1923.747 kb on + strand at 1923.747 kb on + strand at 1923.748 kb on - strand at 1923.748 kb on - strand at 1923.748 kb on - strand at 1923.748 kb on - strand at 1923.775 kb on + strand at 1923.776 kb on - strand at 1923.776 kb on - strand at 1923.776 kb on - strand at 1923.776 kb on - strand at 1923.777 kb on + strand at 1923.777 kb on + strand at 1923.777 kb on + strand at 1923.778 kb on - strand at 1923.781 kb on + strand at 1923.798 kb on + strand at 1923.798 kb on + strand at 1923.799 kb on - strand at 1923.888 kb on - strand at 1923.897 kb on + strand at 1923.898 kb on - strand at 1923.898 kb on - strand at 1923.928 kb on + strand, within PS417_08845 at 1924.159 kb on + strand, within PS417_08845 at 1924.159 kb on + strand, within PS417_08845 at 1924.159 kb on + strand, within PS417_08845 at 1924.160 kb on - strand, within PS417_08845 at 1924.234 kb on + strand at 1924.300 kb on + strand at 1924.300 kb on + strand at 1924.400 kb on - strand at 1924.431 kb on - strand at 1924.827 kb on + strand, within PS417_08850 at 1924.827 kb on + strand, within PS417_08850 at 1924.828 kb on - strand, within PS417_08850 at 1924.884 kb on + strand, within PS417_08850 at 1924.895 kb on - strand, within PS417_08850 at 1924.963 kb on + strand, within PS417_08850 at 1925.027 kb on - strand, within PS417_08850 at 1925.327 kb on - strand, within PS417_08850 at 1925.587 kb on + strand, within PS417_08850 at 1925.588 kb on - strand, within PS417_08850 at 1925.702 kb on - strand at 1925.702 kb on - strand at 1925.702 kb on - strand at 1925.776 kb on + strand at 1925.819 kb on - strand at 1925.862 kb on - strand at 1925.862 kb on - strand at 1926.127 kb on + strand at 1926.127 kb on + strand at 1926.127 kb on + strand at 1926.127 kb on + strand at 1926.128 kb on - strand at 1926.128 kb on - strand at 1926.181 kb on + strand at 1926.181 kb on + strand at 1926.181 kb on + strand at 1926.181 kb on + strand at 1926.182 kb on - strand at 1926.182 kb on - strand at 1926.182 kb on - strand at 1926.185 kb on - strand at 1926.226 kb on - strand at 1926.413 kb on - strand, within PS417_08855 at 1926.543 kb on - strand, within PS417_08855 at 1926.660 kb on - strand, within PS417_08855 at 1926.672 kb on - strand, within PS417_08855 at 1926.821 kb on + strand, within PS417_08855 at 1926.821 kb on + strand, within PS417_08855 at 1926.821 kb on + strand, within PS417_08855
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,923,440 - PS417_08840 0.85 -2.9 1,923,541 + -0.1 1,923,541 + +0.0 1,923,541 + -2.4 1,923,634 + +0.7 1,923,635 - +0.4 1,923,653 + +0.0 1,923,653 + -0.6 1,923,654 - -0.3 1,923,657 + -0.2 1,923,658 - -0.0 1,923,658 - +1.3 1,923,658 - -0.1 1,923,674 + +0.2 1,923,674 + -1.7 1,923,674 + -2.2 1,923,747 + +0.5 1,923,747 + +1.0 1,923,747 + +1.2 1,923,748 - +2.1 1,923,748 - -0.1 1,923,748 - -1.5 1,923,748 - -1.2 1,923,775 + -3.5 1,923,776 - -0.1 1,923,776 - -1.9 1,923,776 - -0.5 1,923,776 - +0.3 1,923,777 + -1.2 1,923,777 + +0.8 1,923,777 + -2.4 1,923,778 - +1.1 1,923,781 + +1.2 1,923,798 + -0.8 1,923,798 + -0.4 1,923,799 - -0.7 1,923,888 - -0.7 1,923,897 + +0.6 1,923,898 - +0.4 1,923,898 - +0.5 1,923,928 + PS417_08845 0.17 -1.6 1,924,159 + PS417_08845 0.80 -0.9 1,924,159 + PS417_08845 0.80 -1.0 1,924,159 + PS417_08845 0.80 -0.3 1,924,160 - PS417_08845 0.80 -0.8 1,924,234 + +0.3 1,924,300 + +0.3 1,924,300 + -0.6 1,924,400 - -0.5 1,924,431 - +0.2 1,924,827 + PS417_08850 0.34 -1.6 1,924,827 + PS417_08850 0.34 -1.4 1,924,828 - PS417_08850 0.34 -0.9 1,924,884 + PS417_08850 0.38 -1.4 1,924,895 - PS417_08850 0.38 -0.2 1,924,963 + PS417_08850 0.43 +0.0 1,925,027 - PS417_08850 0.47 +0.6 1,925,327 - PS417_08850 0.66 -1.4 1,925,587 + PS417_08850 0.83 +0.3 1,925,588 - PS417_08850 0.83 +0.9 1,925,702 - +0.9 1,925,702 - -2.7 1,925,702 - -0.1 1,925,776 + +0.9 1,925,819 - +2.5 1,925,862 - -0.9 1,925,862 - +0.2 1,926,127 + +1.1 1,926,127 + -0.3 1,926,127 + -0.3 1,926,127 + +0.7 1,926,128 - +0.1 1,926,128 - +0.7 1,926,181 + -2.1 1,926,181 + +0.8 1,926,181 + -0.4 1,926,181 + -0.0 1,926,182 - -0.8 1,926,182 - -1.0 1,926,182 - -0.5 1,926,185 - +1.0 1,926,226 - +0.5 1,926,413 - PS417_08855 0.27 -0.2 1,926,543 - PS417_08855 0.42 -3.5 1,926,660 - PS417_08855 0.56 -0.5 1,926,672 - PS417_08855 0.57 -2.9 1,926,821 + PS417_08855 0.75 -1.4 1,926,821 + PS417_08855 0.75 +0.3 1,926,821 + PS417_08855 0.75 -0.9
Or see this region's nucleotide sequence